Previous Infection with SARS-CoV-2 Correlates with Increased Protective Humoral Responses after a Single Dose of an Inactivated COVID-19 Vaccine
Previous studies have indicated that antibody responses can be robustly induced after the vaccination in individuals previously infected by SARS-CoV-2. To evaluate anti-SARS-CoV-2 humoral responses in vaccinated individuals with or without a previous history of COVID-19, we compared levels of anti-S...
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MDPI AG
2022-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/3/510 |
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| author | Flávia F. Bagno Luis A. F. Andrade Sarah A. R. Sérgio Pierina L. Parise Daniel A. Toledo-Teixeira Ricardo T. Gazzinelli Ana P. S. M. Fernandes Santuza M. R. Teixeira Fabiana Granja José L. Proença-Módena Flavio G. da Fonseca |
| author_facet | Flávia F. Bagno Luis A. F. Andrade Sarah A. R. Sérgio Pierina L. Parise Daniel A. Toledo-Teixeira Ricardo T. Gazzinelli Ana P. S. M. Fernandes Santuza M. R. Teixeira Fabiana Granja José L. Proença-Módena Flavio G. da Fonseca |
| author_sort | Flávia F. Bagno |
| collection | DOAJ |
| description | Previous studies have indicated that antibody responses can be robustly induced after the vaccination in individuals previously infected by SARS-CoV-2. To evaluate anti-SARS-CoV-2 humoral responses in vaccinated individuals with or without a previous history of COVID-19, we compared levels of anti-SARS-CoV-2 antibodies in the sera from 21 vaccinees, including COVID-19-recovered or -naïve individuals in different times, before and after immunization with an inactivated COVID-19 vaccine. Anti-SARS-CoV-2-specific antibodies elicited after COVID-19 and/or immunization with an inactivated vaccine were measured by ELISA and Plaque Reduction Neutralizing assays. Antibody kinetics were consistently different between the two vaccine doses for naïve individuals, contrasting with the SARS-CoV-2-recovered subjects in which we observed no additional increase in antibody levels following the second dose. Sera from SARS-CoV2-naïve individuals had no detectable neutralizing activity against lineage B.1 SARS-CoV-2 or Gamma variant five months after the second vaccine dose. Contrarily, SARS-CoV-2-recovered subjects retained considerable neutralizing activity against both viruses. We conclude that a single inactivated SARS-CoV-2 vaccine dose may be sufficient to induce protective antibody responses in individuals with previous history of SARS-CoV-2 infection. |
| first_indexed | 2024-03-09T12:16:51Z |
| format | Article |
| id | doaj.art-72dbb305c9c84ad6b52d8ce92c07f4b1 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:16:51Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-72dbb305c9c84ad6b52d8ce92c07f4b12023-11-30T22:45:35ZengMDPI AGViruses1999-49152022-03-0114351010.3390/v14030510Previous Infection with SARS-CoV-2 Correlates with Increased Protective Humoral Responses after a Single Dose of an Inactivated COVID-19 VaccineFlávia F. Bagno0Luis A. F. Andrade1Sarah A. R. Sérgio2Pierina L. Parise3Daniel A. Toledo-Teixeira4Ricardo T. Gazzinelli5Ana P. S. M. Fernandes6Santuza M. R. Teixeira7Fabiana Granja8José L. Proença-Módena9Flavio G. da Fonseca10Centro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, BrazilCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, BrazilCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, BrazilLaboratory of Emerging Viruses, Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas, Campinas 13083-862, BrazilLaboratory of Emerging Viruses, Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas, Campinas 13083-862, BrazilCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, BrazilCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, BrazilCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, BrazilLaboratory of Emerging Viruses, Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas, Campinas 13083-862, BrazilLaboratory of Emerging Viruses, Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas, Campinas 13083-862, BrazilCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, BrazilPrevious studies have indicated that antibody responses can be robustly induced after the vaccination in individuals previously infected by SARS-CoV-2. To evaluate anti-SARS-CoV-2 humoral responses in vaccinated individuals with or without a previous history of COVID-19, we compared levels of anti-SARS-CoV-2 antibodies in the sera from 21 vaccinees, including COVID-19-recovered or -naïve individuals in different times, before and after immunization with an inactivated COVID-19 vaccine. Anti-SARS-CoV-2-specific antibodies elicited after COVID-19 and/or immunization with an inactivated vaccine were measured by ELISA and Plaque Reduction Neutralizing assays. Antibody kinetics were consistently different between the two vaccine doses for naïve individuals, contrasting with the SARS-CoV-2-recovered subjects in which we observed no additional increase in antibody levels following the second dose. Sera from SARS-CoV2-naïve individuals had no detectable neutralizing activity against lineage B.1 SARS-CoV-2 or Gamma variant five months after the second vaccine dose. Contrarily, SARS-CoV-2-recovered subjects retained considerable neutralizing activity against both viruses. We conclude that a single inactivated SARS-CoV-2 vaccine dose may be sufficient to induce protective antibody responses in individuals with previous history of SARS-CoV-2 infection.https://www.mdpi.com/1999-4915/14/3/510COVID-19CoronaVacantibodiesPRNTELISA |
| spellingShingle | Flávia F. Bagno Luis A. F. Andrade Sarah A. R. Sérgio Pierina L. Parise Daniel A. Toledo-Teixeira Ricardo T. Gazzinelli Ana P. S. M. Fernandes Santuza M. R. Teixeira Fabiana Granja José L. Proença-Módena Flavio G. da Fonseca Previous Infection with SARS-CoV-2 Correlates with Increased Protective Humoral Responses after a Single Dose of an Inactivated COVID-19 Vaccine Viruses COVID-19 CoronaVac antibodies PRNT ELISA |
| title | Previous Infection with SARS-CoV-2 Correlates with Increased Protective Humoral Responses after a Single Dose of an Inactivated COVID-19 Vaccine |
| title_full | Previous Infection with SARS-CoV-2 Correlates with Increased Protective Humoral Responses after a Single Dose of an Inactivated COVID-19 Vaccine |
| title_fullStr | Previous Infection with SARS-CoV-2 Correlates with Increased Protective Humoral Responses after a Single Dose of an Inactivated COVID-19 Vaccine |
| title_full_unstemmed | Previous Infection with SARS-CoV-2 Correlates with Increased Protective Humoral Responses after a Single Dose of an Inactivated COVID-19 Vaccine |
| title_short | Previous Infection with SARS-CoV-2 Correlates with Increased Protective Humoral Responses after a Single Dose of an Inactivated COVID-19 Vaccine |
| title_sort | previous infection with sars cov 2 correlates with increased protective humoral responses after a single dose of an inactivated covid 19 vaccine |
| topic | COVID-19 CoronaVac antibodies PRNT ELISA |
| url | https://www.mdpi.com/1999-4915/14/3/510 |
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