The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of Normothermic Recirculating Isolated Perfused Rat Liver
Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in different tissues. Glycine (Amino acetic acid) is the simplest amino acid incorporated in the structure of proteins. Several investigations indicate the hepatoprotective properties of these amino acids....
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2016-03-01
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| Series: | Trends in Pharmaceutical Sciences |
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| Online Access: | https://tips.sums.ac.ir/article_42185_d0b7e10de1dc07c941563ba4c8c8383d.pdf |
| _version_ | 1819299246612938752 |
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| author | Reza Heidari Akram Jamshidzadeh Hossein Niknahad Farshad Safari Hamdollah Azizi Narges Abdoli Mohammad Mehdi Ommati Forouzan Khodaei Arastoo Saeedi Asma Najibi |
| author_facet | Reza Heidari Akram Jamshidzadeh Hossein Niknahad Farshad Safari Hamdollah Azizi Narges Abdoli Mohammad Mehdi Ommati Forouzan Khodaei Arastoo Saeedi Asma Najibi |
| author_sort | Reza Heidari |
| collection | DOAJ |
| description | Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in different tissues. Glycine (Amino acetic acid) is the simplest amino acid incorporated in the structure of proteins. Several investigations indicate the hepatoprotective properties of these amino acids. On the other hand, antineoplastic agents-induced serum transaminase elevation and liver injury is a clinical complication. The current investigation was designed to screen the possible hepatoprotective properties of taurine and glycine against antineoplastic drugs-induced hepatic injury in an ex vivo model of isolated perfused rat liver. Rat liver was perfused with different concentration (10 µM, 100 µM and 1000 µM) of antineoplastic drugs (Mitoxantrone, Cyclophosphamide, Cisplatin, 5‑Fluorouracil, Doxorubicin and Dacarbazine) via portal vein. Taurine and glycine were administered to drug-treated livers and liver perfusate samples were collected for biochemical measurements (ALT, LDH, AST, and K+). Markers of oxidative stress (reactive oxygen species formation, lipid peroxidation, total antioxidant capacity and glutathione) were also assessed in liver tissue. Antineoplastic drugs caused significant pathological changes in perfusate biochemistry. Furthermore, markers of oxidative stress were significantly elevated in drug‑treated livers. It was found that taurine (5 and 10 mM) and glycine (5 and 10 mM) administration significantly mitigated the biomarkers of liver injury and attenuated drug‑induced oxidative stress. Our data indicate that taurine and glycine supplementation might help as potential therapeutic options to encounter anticancer drugs-induced liver injury. |
| first_indexed | 2024-12-24T05:42:45Z |
| format | Article |
| id | doaj.art-72dc36db46b2454b8cdf15b1080a51e3 |
| institution | Directory Open Access Journal |
| issn | 2423-5652 |
| language | English |
| last_indexed | 2024-12-24T05:42:45Z |
| publishDate | 2016-03-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Trends in Pharmaceutical Sciences |
| spelling | doaj.art-72dc36db46b2454b8cdf15b1080a51e32022-12-21T17:12:46ZengShiraz University of Medical SciencesTrends in Pharmaceutical Sciences2423-56522016-03-0121597642185The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of Normothermic Recirculating Isolated Perfused Rat LiverReza Heidari0Akram Jamshidzadeh1Hossein Niknahad2Farshad Safari3Hamdollah Azizi4Narges Abdoli5Mohammad Mehdi Ommati6Forouzan Khodaei7Arastoo Saeedi8Asma Najibi9Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Pharmacology and Toxicology Department, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Pharmacology-Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.Pharmacology and Toxicology Department, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.Pharmacology and Toxicology Department, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.Food and Drug Organization, Ministry of Health, Tehran, Iran.Center of Experimental & Comparative Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Department of Animal Science, School of Agriculture, Shiraz University, Shiraz, IranPharmacology and Toxicology Department, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.Pharmacology and Toxicology Department, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.Pharmacology and Toxicology Department, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in different tissues. Glycine (Amino acetic acid) is the simplest amino acid incorporated in the structure of proteins. Several investigations indicate the hepatoprotective properties of these amino acids. On the other hand, antineoplastic agents-induced serum transaminase elevation and liver injury is a clinical complication. The current investigation was designed to screen the possible hepatoprotective properties of taurine and glycine against antineoplastic drugs-induced hepatic injury in an ex vivo model of isolated perfused rat liver. Rat liver was perfused with different concentration (10 µM, 100 µM and 1000 µM) of antineoplastic drugs (Mitoxantrone, Cyclophosphamide, Cisplatin, 5‑Fluorouracil, Doxorubicin and Dacarbazine) via portal vein. Taurine and glycine were administered to drug-treated livers and liver perfusate samples were collected for biochemical measurements (ALT, LDH, AST, and K+). Markers of oxidative stress (reactive oxygen species formation, lipid peroxidation, total antioxidant capacity and glutathione) were also assessed in liver tissue. Antineoplastic drugs caused significant pathological changes in perfusate biochemistry. Furthermore, markers of oxidative stress were significantly elevated in drug‑treated livers. It was found that taurine (5 and 10 mM) and glycine (5 and 10 mM) administration significantly mitigated the biomarkers of liver injury and attenuated drug‑induced oxidative stress. Our data indicate that taurine and glycine supplementation might help as potential therapeutic options to encounter anticancer drugs-induced liver injury.https://tips.sums.ac.ir/article_42185_d0b7e10de1dc07c941563ba4c8c8383d.pdfamino acidchemotherapycancerdrug-induced liver injury (dili)hepatoprotectionhepatotoxicity |
| spellingShingle | Reza Heidari Akram Jamshidzadeh Hossein Niknahad Farshad Safari Hamdollah Azizi Narges Abdoli Mohammad Mehdi Ommati Forouzan Khodaei Arastoo Saeedi Asma Najibi The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of Normothermic Recirculating Isolated Perfused Rat Liver Trends in Pharmaceutical Sciences amino acid chemotherapy cancer drug-induced liver injury (dili) hepatoprotection hepatotoxicity |
| title | The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of Normothermic Recirculating Isolated Perfused Rat Liver |
| title_full | The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of Normothermic Recirculating Isolated Perfused Rat Liver |
| title_fullStr | The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of Normothermic Recirculating Isolated Perfused Rat Liver |
| title_full_unstemmed | The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of Normothermic Recirculating Isolated Perfused Rat Liver |
| title_short | The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of Normothermic Recirculating Isolated Perfused Rat Liver |
| title_sort | hepatoprotection provided by taurine and glycine against antineoplastic drugs induced liver injury in an ex vivo model of normothermic recirculating isolated perfused rat liver |
| topic | amino acid chemotherapy cancer drug-induced liver injury (dili) hepatoprotection hepatotoxicity |
| url | https://tips.sums.ac.ir/article_42185_d0b7e10de1dc07c941563ba4c8c8383d.pdf |
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