Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat ()
Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 ge...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Asian-Australasian Association of Animal Production Societies
2012-05-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/25-77.pdf |
| _version_ | 1818422671088549888 |
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| author | X. J. Wang J. J. Shi J. F. Yang Y. Liang Y. F. Wang M. L. Wu S. Y. Li X. D. Guo Z. G. Wang D. J. Liu |
| author_facet | X. J. Wang J. J. Shi J. F. Yang Y. Liang Y. F. Wang M. L. Wu S. Y. Li X. D. Guo Z. G. Wang D. J. Liu |
| author_sort | X. J. Wang |
| collection | DOAJ |
| description | Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal’s fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells. |
| first_indexed | 2024-12-14T13:29:57Z |
| format | Article |
| id | doaj.art-72dc61137805440ab43575f93be4ff8f |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-14T13:29:57Z |
| publishDate | 2012-05-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-72dc61137805440ab43575f93be4ff8f2022-12-21T22:59:43ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172012-05-0125560661210.5713/ajas.2011.1129022720Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat ()X. J. Wang0J. J. Shi1J. F. Yang2Y. Liang3Y. F. Wang4M. L. Wu5S. Y. Li6X. D. Guo7Z. G. Wang8D. J. Liu9 College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, ChinaInsulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal’s fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.http://www.ajas.info/upload/pdf/25-77.pdfCashmere GoatExpression PatternTissue-specificHairGene |
| spellingShingle | X. J. Wang J. J. Shi J. F. Yang Y. Liang Y. F. Wang M. L. Wu S. Y. Li X. D. Guo Z. G. Wang D. J. Liu Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat () Asian-Australasian Journal of Animal Sciences Cashmere Goat Expression Pattern Tissue-specific Hair Gene |
| title | Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat () |
| title_full | Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat () |
| title_fullStr | Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat () |
| title_full_unstemmed | Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat () |
| title_short | Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat () |
| title_sort | molecular characterization and expression pattern of gene igfbp 5 in the cashmere goat |
| topic | Cashmere Goat Expression Pattern Tissue-specific Hair Gene |
| url | http://www.ajas.info/upload/pdf/25-77.pdf |
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