Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis
Background: The aim of this study was to analyze possible interactions of different xenogeneic collagen membranes (CM) and platelet-rich fibrin (PRF). PH values were evaluated in the CM rehydration process with PRF, and their influence on angiogenesis was analyzed in vivo. Materials and Methods: Por...
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MDPI AG
2022-03-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/3/706 |
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| author | Sebastian Blatt Saskia-Vanessa Schröger Andreas Pabst Peer W. Kämmerer Keyvan Sagheb Bilal Al-Nawas |
| author_facet | Sebastian Blatt Saskia-Vanessa Schröger Andreas Pabst Peer W. Kämmerer Keyvan Sagheb Bilal Al-Nawas |
| author_sort | Sebastian Blatt |
| collection | DOAJ |
| description | Background: The aim of this study was to analyze possible interactions of different xenogeneic collagen membranes (CM) and platelet-rich fibrin (PRF). PH values were evaluated in the CM rehydration process with PRF, and their influence on angiogenesis was analyzed in vivo. Materials and Methods: Porcine (Bio-Gide<sup>®</sup>, Geistlich)- and bovine-derived collagen membranes (Symbios<sup>®</sup>, Dentsply Sirona) were biofunctionalized with PRF by plotting process. PRF in comparison to blood, saline and a puffer pH7 solution was analysed for pH-value changes in CM rehydration process in vitro. The yolk sac membrane (YSM) model was used to investigate pro-angiogenic effects of the combination of PRF and the respective CM in comparison to native pendant by vessel in-growth and branching points after 24, 48 and 72 h evaluated light-microscopically and by immunohistochemical staining (CD105, αSMA) in vivo. Results: Significantly higher pH values were found at all points in time in PRF alone and its combined variants with Bio-Gide<sup>®</sup> and Symbios<sup>®</sup> compared with pure native saline solution and pH 7 solution, as well as saline with Symbios<sup>®</sup> and Bio-Gide<sup>®</sup> (each <i>p</i> < 0.01). In the YSM, vessel number and branching points showed no significant differences at 24 and 48 h between all groups (each <i>p</i> > 0.05). For PRF alone, a significantly increased vessel number and branching points between 24 and 48 h (each <i>p</i> < 0.05) and between 24 and 72 h (each <i>p</i> < 0.05) was shown. After 72 h, CM in combination with PRF induced a statistically significant addition to vessels and branching points in comparison with native YSM (<i>p</i> < 0.01) but not vs. its native pendants (<i>p</i> > 0.05). Summary: PRF represents a promising alternative for CM rehydration to enhance CM vascularization. |
| first_indexed | 2024-03-09T20:04:50Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T20:04:50Z |
| publishDate | 2022-03-01 |
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| spelling | doaj.art-72de7258000d404ab0e57b6bcdb435fd2023-11-24T00:33:55ZengMDPI AGBiomedicines2227-90592022-03-0110370610.3390/biomedicines10030706Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and AngiogenesisSebastian Blatt0Saskia-Vanessa Schröger1Andreas Pabst2Peer W. Kämmerer3Keyvan Sagheb4Bilal Al-Nawas5Department of Oral and Maxillofacial Surgery—Plastic Operations, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Oral and Maxillofacial Surgery—Plastic Operations, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Oral and Maxillofacial Surgery, Federal Armed Forces Hospital, Rübenacherstr. 170, 56072 Koblenz, GermanyDepartment of Oral and Maxillofacial Surgery—Plastic Operations, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Oral and Maxillofacial Surgery—Plastic Operations, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Oral and Maxillofacial Surgery—Plastic Operations, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyBackground: The aim of this study was to analyze possible interactions of different xenogeneic collagen membranes (CM) and platelet-rich fibrin (PRF). PH values were evaluated in the CM rehydration process with PRF, and their influence on angiogenesis was analyzed in vivo. Materials and Methods: Porcine (Bio-Gide<sup>®</sup>, Geistlich)- and bovine-derived collagen membranes (Symbios<sup>®</sup>, Dentsply Sirona) were biofunctionalized with PRF by plotting process. PRF in comparison to blood, saline and a puffer pH7 solution was analysed for pH-value changes in CM rehydration process in vitro. The yolk sac membrane (YSM) model was used to investigate pro-angiogenic effects of the combination of PRF and the respective CM in comparison to native pendant by vessel in-growth and branching points after 24, 48 and 72 h evaluated light-microscopically and by immunohistochemical staining (CD105, αSMA) in vivo. Results: Significantly higher pH values were found at all points in time in PRF alone and its combined variants with Bio-Gide<sup>®</sup> and Symbios<sup>®</sup> compared with pure native saline solution and pH 7 solution, as well as saline with Symbios<sup>®</sup> and Bio-Gide<sup>®</sup> (each <i>p</i> < 0.01). In the YSM, vessel number and branching points showed no significant differences at 24 and 48 h between all groups (each <i>p</i> > 0.05). For PRF alone, a significantly increased vessel number and branching points between 24 and 48 h (each <i>p</i> < 0.05) and between 24 and 72 h (each <i>p</i> < 0.05) was shown. After 72 h, CM in combination with PRF induced a statistically significant addition to vessels and branching points in comparison with native YSM (<i>p</i> < 0.01) but not vs. its native pendants (<i>p</i> > 0.05). Summary: PRF represents a promising alternative for CM rehydration to enhance CM vascularization.https://www.mdpi.com/2227-9059/10/3/706platelet-rich fibrincollagen matrixin ovo yolk sacangiogenesispH valueguided bone regeneration |
| spellingShingle | Sebastian Blatt Saskia-Vanessa Schröger Andreas Pabst Peer W. Kämmerer Keyvan Sagheb Bilal Al-Nawas Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis Biomedicines platelet-rich fibrin collagen matrix in ovo yolk sac angiogenesis pH value guided bone regeneration |
| title | Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis |
| title_full | Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis |
| title_fullStr | Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis |
| title_full_unstemmed | Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis |
| title_short | Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis |
| title_sort | biofunctionalization of xenogeneic collagen membranes with autologous platelet concentrate influence on rehydration protocol and angiogenesis |
| topic | platelet-rich fibrin collagen matrix in ovo yolk sac angiogenesis pH value guided bone regeneration |
| url | https://www.mdpi.com/2227-9059/10/3/706 |
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