Distinct immune- and defense-related molecular fingerprints in sepatated coelomocyte subsets of Eisenia andrei earthworms
During phylogenesis different types of immunocytes such as amoebocytes and eleocytes have developed in earthworms to defend the host against microbial pathogens. Previously we applied a cell sorting-based approach to untangle the morphological and functional properties of these aforementioned coelom...
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2018-09-01
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| Series: | Invertebrate Survival Journal |
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| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/442 |
| _version_ | 1818866144029704192 |
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| author | K Bodó D Ernszt P Németh P Engelmann |
| author_facet | K Bodó D Ernszt P Németh P Engelmann |
| author_sort | K Bodó |
| collection | DOAJ |
| description | During phylogenesis different types of immunocytes such as amoebocytes and eleocytes have developed in earthworms to defend the host against microbial pathogens. Previously we applied a cell sorting-based approach to untangle the morphological and functional properties of these aforementioned coelomocyte subsets. In order to compare their constitutive gene expression patterns, cell-sorting was performed and followed by semiquantitative RT-PCR in the distinct, separated coelomocyte subpopulations of unmanipulated Eisenia andrei earthworms. We targeted a variety of genes with diverse functions ranging from pattern recognition through intracellular signaling to oxidative stress. Several immune-related genes (CCF, TLR, lumbricin, LuRP, MyD88) were only manifested in the amoebocytes. In contrast, other immune response genes (lysozyme, lysenin), lysosomal hydrolases (cathepsin L and cathepsin C) and cystatin B were expressed in both subpopulations. In addition, cell signaling molecules (MyD88, PKC1) and oxidative stress-related genes (Cu/ZnSOD, MnSOD) were mainly observed in amoebocytes, while other stress-related genes (Cd-metallothionein, catalase) were apparent in both subsets. We conclude that these characteristic differences of the molecular signatures manifest in the functional heterogeneity of distinct coelomocyte subtypes. |
| first_indexed | 2024-12-19T10:58:46Z |
| format | Article |
| id | doaj.art-72de77904a484a93816c5e970e63a1b8 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-19T10:58:46Z |
| publishDate | 2018-09-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-72de77904a484a93816c5e970e63a1b82022-12-21T20:24:42ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2018-09-01151Distinct immune- and defense-related molecular fingerprints in sepatated coelomocyte subsets of Eisenia andrei earthwormsK Bodó0D Ernszt1P Németh2P Engelmann3Department of Immunology and Biotechnology, Clinical Center, Medical School, University of Pécs H-7643, Szigeti u. 12, HungaryDepartment of Physiology, Medical School, University of Pécs H-7643, Szigeti u. 12, HungaryDepartment of Immunology and Biotechnology, Clinical Center, Medical School, University of Pécs H-7643, Szigeti u. 12, HungaryDepartment of Immunology and Biotechnology, Clinical Center, Medical School, University of Pécs H-7643, Szigeti u. 12, HungaryDuring phylogenesis different types of immunocytes such as amoebocytes and eleocytes have developed in earthworms to defend the host against microbial pathogens. Previously we applied a cell sorting-based approach to untangle the morphological and functional properties of these aforementioned coelomocyte subsets. In order to compare their constitutive gene expression patterns, cell-sorting was performed and followed by semiquantitative RT-PCR in the distinct, separated coelomocyte subpopulations of unmanipulated Eisenia andrei earthworms. We targeted a variety of genes with diverse functions ranging from pattern recognition through intracellular signaling to oxidative stress. Several immune-related genes (CCF, TLR, lumbricin, LuRP, MyD88) were only manifested in the amoebocytes. In contrast, other immune response genes (lysozyme, lysenin), lysosomal hydrolases (cathepsin L and cathepsin C) and cystatin B were expressed in both subpopulations. In addition, cell signaling molecules (MyD88, PKC1) and oxidative stress-related genes (Cu/ZnSOD, MnSOD) were mainly observed in amoebocytes, while other stress-related genes (Cd-metallothionein, catalase) were apparent in both subsets. We conclude that these characteristic differences of the molecular signatures manifest in the functional heterogeneity of distinct coelomocyte subtypes.https://isj02.unimore.it/index.php/ISJ/article/view/442Eisenia andreicoelomocytescell sortinggene expressionimmune responseoxidative stress |
| spellingShingle | K Bodó D Ernszt P Németh P Engelmann Distinct immune- and defense-related molecular fingerprints in sepatated coelomocyte subsets of Eisenia andrei earthworms Invertebrate Survival Journal Eisenia andrei coelomocytes cell sorting gene expression immune response oxidative stress |
| title | Distinct immune- and defense-related molecular fingerprints in sepatated coelomocyte subsets of Eisenia andrei earthworms |
| title_full | Distinct immune- and defense-related molecular fingerprints in sepatated coelomocyte subsets of Eisenia andrei earthworms |
| title_fullStr | Distinct immune- and defense-related molecular fingerprints in sepatated coelomocyte subsets of Eisenia andrei earthworms |
| title_full_unstemmed | Distinct immune- and defense-related molecular fingerprints in sepatated coelomocyte subsets of Eisenia andrei earthworms |
| title_short | Distinct immune- and defense-related molecular fingerprints in sepatated coelomocyte subsets of Eisenia andrei earthworms |
| title_sort | distinct immune and defense related molecular fingerprints in sepatated coelomocyte subsets of eisenia andrei earthworms |
| topic | Eisenia andrei coelomocytes cell sorting gene expression immune response oxidative stress |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/442 |
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