Gestational arsenic exposure induces anxiety-like behaviors in F1 female mice by dysregulation of neurological and immunological markers
Background: Arsenic is a harmful heavy metal and a well-known developmental neurotoxicant. Previously, we have reported that gestational arsenic exposure resulted in impaired social behaviors in F1 and F2 male mice. However, little is known about the developmental arsenic exposure on anxiety-like be...
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| Format: | Article |
| Language: | English |
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Komiyama Printing Co. Ltd
2023-07-01
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| Series: | Environmental Health and Preventive Medicine |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/ehpm/28/0/28_23-00046/_html/-char/en |
| _version_ | 1797771301476106240 |
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| author | Chaw Kyi-Tha-Thu Soe-Minn Htway Takehiro Suzuki Keiko Nohara Tin-Tin Win-Shwe |
| author_facet | Chaw Kyi-Tha-Thu Soe-Minn Htway Takehiro Suzuki Keiko Nohara Tin-Tin Win-Shwe |
| author_sort | Chaw Kyi-Tha-Thu |
| collection | DOAJ |
| description | Background: Arsenic is a harmful heavy metal and a well-known developmental neurotoxicant. Previously, we have reported that gestational arsenic exposure resulted in impaired social behaviors in F1 and F2 male mice. However, little is known about the developmental arsenic exposure on anxiety-like behavior. This study aimed to detect the effect of gestational arsenic exposure on anxiety-like behavior and related gene expressions in 74-week-old F1 female mice. Method: Pregnant C3H/HeN mice (F0) were given drinking water containing 85 ppm sodium arsenite (NaAsO2) from gestational day 8 to 18. The control mice were given tap water only. At 74-week-old, open field test was performed, then anxiety and apoptosis-related factors were determined by real_time RT_PCR and immunohistochemical analyses. Results: The arsenite-exposed F1 female mice showed decreased center entry and center time in open field test. In addition, the number of grooming and fecal pallet was significantly increased in the arsenite-exposed F1 female mice compared to the control. Downregulation of brain-derived neurotrophic factor (BDNF), serotonin receptor (5HT1A) and upregulation of nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), interleukin 1 β (IL-1β), cyclooxygenase 2 (COX2), caspase-3, Bcl2-associated X protein (Bax) were detected in the prefrontal cortex in the arsenite-exposed F1 female mice. Microglial marker ionized calcium-binding adapter molecule 1 (Iba1)-positive cells were increased in the arsenite-exposed F1 female mice. Moreover, a significantly increased plasma corticosterone level was observed in the arsenic-exposed F1 female mice. Conclusions: This study suggested that gestational arsenic exposure induced anxiety-like behavior accompanied with dysregulation of neurological and immunological markers, neuroinflammatory responses, neuronal apoptosis, and decreased neurogenesis in the prefrontal cortex of F1 female mice. |
| first_indexed | 2024-03-12T21:35:32Z |
| format | Article |
| id | doaj.art-72def14fde9c4b9f86c5a6dbe704474f |
| institution | Directory Open Access Journal |
| issn | 1342-078X 1347-4715 |
| language | English |
| last_indexed | 2024-03-12T21:35:32Z |
| publishDate | 2023-07-01 |
| publisher | Komiyama Printing Co. Ltd |
| record_format | Article |
| series | Environmental Health and Preventive Medicine |
| spelling | doaj.art-72def14fde9c4b9f86c5a6dbe704474f2023-07-27T07:49:25ZengKomiyama Printing Co. LtdEnvironmental Health and Preventive Medicine1342-078X1347-47152023-07-0128434310.1265/ehpm.23-00046ehpmGestational arsenic exposure induces anxiety-like behaviors in F1 female mice by dysregulation of neurological and immunological markersChaw Kyi-Tha-Thu0Soe-Minn Htway1Takehiro Suzuki2Keiko Nohara3Tin-Tin Win-Shwe4Department of Immunology, International University of Health and Welfare School of Medicine, Narita CampusDepartment of Physiology, University of Medicine, MagwayHealth and Environmental Risk Division, National Institute for Environmental StudiesHealth and Environmental Risk Division, National Institute for Environmental StudiesHealth and Environmental Risk Division, National Institute for Environmental StudiesBackground: Arsenic is a harmful heavy metal and a well-known developmental neurotoxicant. Previously, we have reported that gestational arsenic exposure resulted in impaired social behaviors in F1 and F2 male mice. However, little is known about the developmental arsenic exposure on anxiety-like behavior. This study aimed to detect the effect of gestational arsenic exposure on anxiety-like behavior and related gene expressions in 74-week-old F1 female mice. Method: Pregnant C3H/HeN mice (F0) were given drinking water containing 85 ppm sodium arsenite (NaAsO2) from gestational day 8 to 18. The control mice were given tap water only. At 74-week-old, open field test was performed, then anxiety and apoptosis-related factors were determined by real_time RT_PCR and immunohistochemical analyses. Results: The arsenite-exposed F1 female mice showed decreased center entry and center time in open field test. In addition, the number of grooming and fecal pallet was significantly increased in the arsenite-exposed F1 female mice compared to the control. Downregulation of brain-derived neurotrophic factor (BDNF), serotonin receptor (5HT1A) and upregulation of nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), interleukin 1 β (IL-1β), cyclooxygenase 2 (COX2), caspase-3, Bcl2-associated X protein (Bax) were detected in the prefrontal cortex in the arsenite-exposed F1 female mice. Microglial marker ionized calcium-binding adapter molecule 1 (Iba1)-positive cells were increased in the arsenite-exposed F1 female mice. Moreover, a significantly increased plasma corticosterone level was observed in the arsenic-exposed F1 female mice. Conclusions: This study suggested that gestational arsenic exposure induced anxiety-like behavior accompanied with dysregulation of neurological and immunological markers, neuroinflammatory responses, neuronal apoptosis, and decreased neurogenesis in the prefrontal cortex of F1 female mice.https://www.jstage.jst.go.jp/article/ehpm/28/0/28_23-00046/_html/-char/enanxietyarsenicgestational exposuref1 female miceneurotransmitterapoptosis |
| spellingShingle | Chaw Kyi-Tha-Thu Soe-Minn Htway Takehiro Suzuki Keiko Nohara Tin-Tin Win-Shwe Gestational arsenic exposure induces anxiety-like behaviors in F1 female mice by dysregulation of neurological and immunological markers Environmental Health and Preventive Medicine anxiety arsenic gestational exposure f1 female mice neurotransmitter apoptosis |
| title | Gestational arsenic exposure induces anxiety-like behaviors in F1 female mice by dysregulation of neurological and immunological markers |
| title_full | Gestational arsenic exposure induces anxiety-like behaviors in F1 female mice by dysregulation of neurological and immunological markers |
| title_fullStr | Gestational arsenic exposure induces anxiety-like behaviors in F1 female mice by dysregulation of neurological and immunological markers |
| title_full_unstemmed | Gestational arsenic exposure induces anxiety-like behaviors in F1 female mice by dysregulation of neurological and immunological markers |
| title_short | Gestational arsenic exposure induces anxiety-like behaviors in F1 female mice by dysregulation of neurological and immunological markers |
| title_sort | gestational arsenic exposure induces anxiety like behaviors in f1 female mice by dysregulation of neurological and immunological markers |
| topic | anxiety arsenic gestational exposure f1 female mice neurotransmitter apoptosis |
| url | https://www.jstage.jst.go.jp/article/ehpm/28/0/28_23-00046/_html/-char/en |
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