Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations
A spectrophotometric method for the determination of trace amounts of chloramphenicol has been proposed. This method depends upon the reduction of the nitro to amino group, condensation with promethazine reagent in the presence of cerium (IV) ions to form a colored dye which exhibits maximum absorpt...
| Main Authors: | , |
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| Format: | Article |
| Language: | Arabic |
| Published: |
College of Education for Pure Sciences
2018-06-01
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| Series: | مجلة التربية والعلم |
| Subjects: | |
| Online Access: | https://edusj.mosuljournals.com/article_159308_7443272d1ae24d3a504ad63627762a34.pdf |
| _version_ | 1818258619885420544 |
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| author | Tamathir A. Hamoudi Wadala A. Bashir |
| author_facet | Tamathir A. Hamoudi Wadala A. Bashir |
| author_sort | Tamathir A. Hamoudi |
| collection | DOAJ |
| description | A spectrophotometric method for the determination of trace amounts of chloramphenicol has been proposed. This method depends upon the reduction of the nitro to amino group, condensation with promethazine reagent in the presence of cerium (IV) ions to form a colored dye which exhibits maximum absorption at 606 nm with a high sensitivity (molar absorptivity is 1.292×104 l.mol-1.cm-1, and Sandell's sensitivity index of 0.025 μg/cm2). Beer' s law is applied within the concentration range of obeyed in 0.4 to 12 ppm with a relative error of -2.73 to + 0.37% and a relative standard deviation of ± 3.84 to ± 0.44%, depending on the concentration level. The method has been successfully applied to the determination of chloramphenicol in pharmaceutical preparations (capsules, eye drop and eye ointment). |
| first_indexed | 2024-12-12T18:02:26Z |
| format | Article |
| id | doaj.art-72e70298beb948ff9d87f30dcc9780ef |
| institution | Directory Open Access Journal |
| issn | 1812-125X 2664-2530 |
| language | Arabic |
| last_indexed | 2024-12-12T18:02:26Z |
| publishDate | 2018-06-01 |
| publisher | College of Education for Pure Sciences |
| record_format | Article |
| series | مجلة التربية والعلم |
| spelling | doaj.art-72e70298beb948ff9d87f30dcc9780ef2022-12-22T00:16:35ZaraCollege of Education for Pure Sciencesمجلة التربية والعلم1812-125X2664-25302018-06-01273193510.33899/edusj.2018.159308159308Spectrophotometric Determination of Chloramphenicol in Pharmaceutical PreparationsTamathir A. HamoudiWadala A. BashirA spectrophotometric method for the determination of trace amounts of chloramphenicol has been proposed. This method depends upon the reduction of the nitro to amino group, condensation with promethazine reagent in the presence of cerium (IV) ions to form a colored dye which exhibits maximum absorption at 606 nm with a high sensitivity (molar absorptivity is 1.292×104 l.mol-1.cm-1, and Sandell's sensitivity index of 0.025 μg/cm2). Beer' s law is applied within the concentration range of obeyed in 0.4 to 12 ppm with a relative error of -2.73 to + 0.37% and a relative standard deviation of ± 3.84 to ± 0.44%, depending on the concentration level. The method has been successfully applied to the determination of chloramphenicol in pharmaceutical preparations (capsules, eye drop and eye ointment).https://edusj.mosuljournals.com/article_159308_7443272d1ae24d3a504ad63627762a34.pdfchloramphenicolpharmaceutical preparationscerium |
| spellingShingle | Tamathir A. Hamoudi Wadala A. Bashir Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations مجلة التربية والعلم chloramphenicol pharmaceutical preparations cerium |
| title | Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations |
| title_full | Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations |
| title_fullStr | Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations |
| title_full_unstemmed | Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations |
| title_short | Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations |
| title_sort | spectrophotometric determination of chloramphenicol in pharmaceutical preparations |
| topic | chloramphenicol pharmaceutical preparations cerium |
| url | https://edusj.mosuljournals.com/article_159308_7443272d1ae24d3a504ad63627762a34.pdf |
| work_keys_str_mv | AT tamathirahamoudi spectrophotometricdeterminationofchloramphenicolinpharmaceuticalpreparations AT wadalaabashir spectrophotometricdeterminationofchloramphenicolinpharmaceuticalpreparations |