Modulation efficiency of clove oil nano-emulsion against genotoxic, oxidative stress, and histological injuries induced via titanium dioxide nanoparticles in mice
Abstract Titanium dioxide nanoparticles (TiO2-NPs) have found wide applications in medical and industrial fields. However, the toxic effect of various tissues is still under study. In this study, we evaluated the toxic effect of TiO2-NP on stomach, liver, and kidney tissues and the amelioration effe...
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| Format: | Article |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-57728-1 |
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| author | Hanan R. H. Mohamed Sawsan El-Shamy Sherein S. Abdelgayed Rofida Albash Haidan El-Shorbagy |
| author_facet | Hanan R. H. Mohamed Sawsan El-Shamy Sherein S. Abdelgayed Rofida Albash Haidan El-Shorbagy |
| author_sort | Hanan R. H. Mohamed |
| collection | DOAJ |
| description | Abstract Titanium dioxide nanoparticles (TiO2-NPs) have found wide applications in medical and industrial fields. However, the toxic effect of various tissues is still under study. In this study, we evaluated the toxic effect of TiO2-NP on stomach, liver, and kidney tissues and the amelioration effect of clove oil nanoemulsion (CLV-NE) against DNA damage, oxidative stress, pathological changes, and the apoptotic effect of TiO2-NPs. Four groups of male mice were subjected to oral treatment for five consecutive days including, the control group, the group treated with TiO2-NPs (50 mg/kg), the group treated with (CLV-NE) (5% of the MTD), and the group treated with TiO2-NPs plus CLV-NE. The results revealed that the treatment with TiO2-NPs significantly caused DNA damage in the liver, stomach, and kidney tissues due to increased ROS as indicated by the reduction of the antioxidant activity of SOD and Gpx and increased MDA level. Further, abnormal histological signs and apoptotic effect confirmed by the significant elevation of p53 expression were reported after TiO2-NPs administration. The present data reported a significant improvement in the previous parameters after treatment with CLV-NE. These results showed the collaborative effect of the oils and the extra role of nanoemulsion in enhancing antioxidant effectiveness that enhances its disperse-ability and further promotes its controlled release. One could conclude that CLV-NE is safe and can be used as a powerful antioxidative agent to assess the toxic effects of the acute use of TiO2-NPs. |
| first_indexed | 2024-04-24T12:40:32Z |
| format | Article |
| id | doaj.art-72e728acf25a4263aa5457e3c616cf81 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T12:40:32Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-72e728acf25a4263aa5457e3c616cf812024-04-07T11:17:28ZengNature PortfolioScientific Reports2045-23222024-04-0114111010.1038/s41598-024-57728-1Modulation efficiency of clove oil nano-emulsion against genotoxic, oxidative stress, and histological injuries induced via titanium dioxide nanoparticles in miceHanan R. H. Mohamed0Sawsan El-Shamy1Sherein S. Abdelgayed2Rofida Albash3Haidan El-Shorbagy4Zoology Department, Faculty of Science, Cairo UniversityCollege of Oral and Dental Surgery, Misr University for Science and TechnologyPathology Department, Faculty of Veterinary Medicine Cairo University GizaCollege of Oral and Dental Surgery, Misr University for Science and TechnologyZoology Department, Faculty of Science, Cairo UniversityAbstract Titanium dioxide nanoparticles (TiO2-NPs) have found wide applications in medical and industrial fields. However, the toxic effect of various tissues is still under study. In this study, we evaluated the toxic effect of TiO2-NP on stomach, liver, and kidney tissues and the amelioration effect of clove oil nanoemulsion (CLV-NE) against DNA damage, oxidative stress, pathological changes, and the apoptotic effect of TiO2-NPs. Four groups of male mice were subjected to oral treatment for five consecutive days including, the control group, the group treated with TiO2-NPs (50 mg/kg), the group treated with (CLV-NE) (5% of the MTD), and the group treated with TiO2-NPs plus CLV-NE. The results revealed that the treatment with TiO2-NPs significantly caused DNA damage in the liver, stomach, and kidney tissues due to increased ROS as indicated by the reduction of the antioxidant activity of SOD and Gpx and increased MDA level. Further, abnormal histological signs and apoptotic effect confirmed by the significant elevation of p53 expression were reported after TiO2-NPs administration. The present data reported a significant improvement in the previous parameters after treatment with CLV-NE. These results showed the collaborative effect of the oils and the extra role of nanoemulsion in enhancing antioxidant effectiveness that enhances its disperse-ability and further promotes its controlled release. One could conclude that CLV-NE is safe and can be used as a powerful antioxidative agent to assess the toxic effects of the acute use of TiO2-NPs.https://doi.org/10.1038/s41598-024-57728-1Titanium dioxide nanoparticleClove oil nanoemulsionTitanium dioxide nanoparticles toxicityOxidative stress |
| spellingShingle | Hanan R. H. Mohamed Sawsan El-Shamy Sherein S. Abdelgayed Rofida Albash Haidan El-Shorbagy Modulation efficiency of clove oil nano-emulsion against genotoxic, oxidative stress, and histological injuries induced via titanium dioxide nanoparticles in mice Scientific Reports Titanium dioxide nanoparticle Clove oil nanoemulsion Titanium dioxide nanoparticles toxicity Oxidative stress |
| title | Modulation efficiency of clove oil nano-emulsion against genotoxic, oxidative stress, and histological injuries induced via titanium dioxide nanoparticles in mice |
| title_full | Modulation efficiency of clove oil nano-emulsion against genotoxic, oxidative stress, and histological injuries induced via titanium dioxide nanoparticles in mice |
| title_fullStr | Modulation efficiency of clove oil nano-emulsion against genotoxic, oxidative stress, and histological injuries induced via titanium dioxide nanoparticles in mice |
| title_full_unstemmed | Modulation efficiency of clove oil nano-emulsion against genotoxic, oxidative stress, and histological injuries induced via titanium dioxide nanoparticles in mice |
| title_short | Modulation efficiency of clove oil nano-emulsion against genotoxic, oxidative stress, and histological injuries induced via titanium dioxide nanoparticles in mice |
| title_sort | modulation efficiency of clove oil nano emulsion against genotoxic oxidative stress and histological injuries induced via titanium dioxide nanoparticles in mice |
| topic | Titanium dioxide nanoparticle Clove oil nanoemulsion Titanium dioxide nanoparticles toxicity Oxidative stress |
| url | https://doi.org/10.1038/s41598-024-57728-1 |
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