Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration
Cell fate decisions are governed by a complex interplay between cell-autonomous signals and stimuli from the surrounding tissue. In vivo cells are connected to their neighbors and to the extracellular matrix forming a complex three-dimensional (3-D) microenvironment that is not reproduced in convent...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2005-10-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000002 |
| _version_ | 1819115841079214080 |
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| author | M.I.D. Rossi A.P.D.N. Barros L.S. Baptista L.R. Garzoni M.N. Meirelles C.M. Takiya B.M.O. Pascarelli H.S. Dutra R. Borojevic |
| author_facet | M.I.D. Rossi A.P.D.N. Barros L.S. Baptista L.R. Garzoni M.N. Meirelles C.M. Takiya B.M.O. Pascarelli H.S. Dutra R. Borojevic |
| author_sort | M.I.D. Rossi |
| collection | DOAJ |
| description | Cell fate decisions are governed by a complex interplay between cell-autonomous signals and stimuli from the surrounding tissue. In vivo cells are connected to their neighbors and to the extracellular matrix forming a complex three-dimensional (3-D) microenvironment that is not reproduced in conventional in vitro systems. A large body of evidence indicates that mechanical tension applied to the cytoskeleton controls cell proliferation, differentiation and migration, suggesting that 3-D in vitro culture systems that mimic the in vivo situation would reveal biological subtleties. In hematopoietic tissues, the microenvironment plays a crucial role in stem and progenitor cell survival, differentiation, proliferation, and migration. In adults, hematopoiesis takes place inside the bone marrow cavity where hematopoietic cells are intimately associated with a specialized three 3-D scaffold of stromal cell surfaces and extracellular matrix that comprise specific niches. The relationship between hematopoietic cells and their niches is highly dynamic. Under steady-state conditions, hematopoietic cells migrate within the marrow cavity and circulate in the bloodstream. The mechanisms underlying hematopoietic stem/progenitor cell homing and mobilization have been studied in animal models, since conventional two-dimensional (2-D) bone marrow cell cultures do not reproduce the complex 3-D environment. In this review, we will highlight some of the mechanisms controlling hematopoietic cell migration and 3-D culture systems. |
| first_indexed | 2024-12-22T05:07:36Z |
| format | Article |
| id | doaj.art-72e7a199fd1a45089e003535b2fc96b3 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-22T05:07:36Z |
| publishDate | 2005-10-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-72e7a199fd1a45089e003535b2fc96b32022-12-21T18:38:04ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2005-10-0138101455146210.1590/S0100-879X2005001000002Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migrationM.I.D. RossiA.P.D.N. BarrosL.S. BaptistaL.R. GarzoniM.N. MeirellesC.M. TakiyaB.M.O. PascarelliH.S. DutraR. BorojevicCell fate decisions are governed by a complex interplay between cell-autonomous signals and stimuli from the surrounding tissue. In vivo cells are connected to their neighbors and to the extracellular matrix forming a complex three-dimensional (3-D) microenvironment that is not reproduced in conventional in vitro systems. A large body of evidence indicates that mechanical tension applied to the cytoskeleton controls cell proliferation, differentiation and migration, suggesting that 3-D in vitro culture systems that mimic the in vivo situation would reveal biological subtleties. In hematopoietic tissues, the microenvironment plays a crucial role in stem and progenitor cell survival, differentiation, proliferation, and migration. In adults, hematopoiesis takes place inside the bone marrow cavity where hematopoietic cells are intimately associated with a specialized three 3-D scaffold of stromal cell surfaces and extracellular matrix that comprise specific niches. The relationship between hematopoietic cells and their niches is highly dynamic. Under steady-state conditions, hematopoietic cells migrate within the marrow cavity and circulate in the bloodstream. The mechanisms underlying hematopoietic stem/progenitor cell homing and mobilization have been studied in animal models, since conventional two-dimensional (2-D) bone marrow cell cultures do not reproduce the complex 3-D environment. In this review, we will highlight some of the mechanisms controlling hematopoietic cell migration and 3-D culture systems.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000002Multicellular spheroidThree-dimensional cultureCell migrationBone marrow |
| spellingShingle | M.I.D. Rossi A.P.D.N. Barros L.S. Baptista L.R. Garzoni M.N. Meirelles C.M. Takiya B.M.O. Pascarelli H.S. Dutra R. Borojevic Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration Brazilian Journal of Medical and Biological Research Multicellular spheroid Three-dimensional culture Cell migration Bone marrow |
| title | Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration |
| title_full | Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration |
| title_fullStr | Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration |
| title_full_unstemmed | Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration |
| title_short | Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration |
| title_sort | multicellular spheroids of bone marrow stromal cells a three dimensional in vitro culture system for the study of hematopoietic cell migration |
| topic | Multicellular spheroid Three-dimensional culture Cell migration Bone marrow |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000002 |
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