Norwalk Virus Shedding after Experimental Human Infection
Noroviruses are the most common cause of viral gastroenteritis in the United States. To determine the magnitude and duration of virus shedding in feces, we evaluated persons who had been experimentally infected with Norwalk virus. Of 16 persons, clinical gastroenteritis (watery diarrhea and/or vomit...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2008-10-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/14/10/08-0117_article |
| _version_ | 1818515136448561152 |
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| author | Robert L. Atmar Antone R. Opekun Mark A. Gilger Mary K. Estes Sue E. Crawford Frederick H. Neill David Y. Graham |
| author_facet | Robert L. Atmar Antone R. Opekun Mark A. Gilger Mary K. Estes Sue E. Crawford Frederick H. Neill David Y. Graham |
| author_sort | Robert L. Atmar |
| collection | DOAJ |
| description | Noroviruses are the most common cause of viral gastroenteritis in the United States. To determine the magnitude and duration of virus shedding in feces, we evaluated persons who had been experimentally infected with Norwalk virus. Of 16 persons, clinical gastroenteritis (watery diarrhea and/or vomiting) developed in 11; symptomatic illness lasted 1–2 days. Virus shedding was first detected by reverse transcription–PCR (RT-PCR) 18 hours after participant inoculation and lasted a median of 28 days after inoculation (range 13–56 days). The median peak amount of virus shedding was 95 × 109 (range 0.5–1,640 ×109) genomic copies/g feces as measured by quantitative RT-PCR. Virus shedding was first detected by antigen ELISA ≈33 hours (median 42 hours) after inoculation and lasted 10 days (median 7 days) after inoculation. Understanding of the relevance of prolonged fecal norovirus excretion must await the development of sensitive methods to measure virus infectivity. |
| first_indexed | 2024-12-11T00:25:02Z |
| format | Article |
| id | doaj.art-72eaa773a32f4b568f0a79f01363e3aa |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-11T00:25:02Z |
| publishDate | 2008-10-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-72eaa773a32f4b568f0a79f01363e3aa2022-12-22T01:27:36ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592008-10-0114101553155710.3201/eid1410.080117Norwalk Virus Shedding after Experimental Human InfectionRobert L. AtmarAntone R. OpekunMark A. GilgerMary K. EstesSue E. CrawfordFrederick H. NeillDavid Y. GrahamNoroviruses are the most common cause of viral gastroenteritis in the United States. To determine the magnitude and duration of virus shedding in feces, we evaluated persons who had been experimentally infected with Norwalk virus. Of 16 persons, clinical gastroenteritis (watery diarrhea and/or vomiting) developed in 11; symptomatic illness lasted 1–2 days. Virus shedding was first detected by reverse transcription–PCR (RT-PCR) 18 hours after participant inoculation and lasted a median of 28 days after inoculation (range 13–56 days). The median peak amount of virus shedding was 95 × 109 (range 0.5–1,640 ×109) genomic copies/g feces as measured by quantitative RT-PCR. Virus shedding was first detected by antigen ELISA ≈33 hours (median 42 hours) after inoculation and lasted 10 days (median 7 days) after inoculation. Understanding of the relevance of prolonged fecal norovirus excretion must await the development of sensitive methods to measure virus infectivity.https://wwwnc.cdc.gov/eid/article/14/10/08-0117_articlenorovirusgastroenteritisRT-PCRNorwalk virusadulthuman |
| spellingShingle | Robert L. Atmar Antone R. Opekun Mark A. Gilger Mary K. Estes Sue E. Crawford Frederick H. Neill David Y. Graham Norwalk Virus Shedding after Experimental Human Infection Emerging Infectious Diseases norovirus gastroenteritis RT-PCR Norwalk virus adult human |
| title | Norwalk Virus Shedding after Experimental Human Infection |
| title_full | Norwalk Virus Shedding after Experimental Human Infection |
| title_fullStr | Norwalk Virus Shedding after Experimental Human Infection |
| title_full_unstemmed | Norwalk Virus Shedding after Experimental Human Infection |
| title_short | Norwalk Virus Shedding after Experimental Human Infection |
| title_sort | norwalk virus shedding after experimental human infection |
| topic | norovirus gastroenteritis RT-PCR Norwalk virus adult human |
| url | https://wwwnc.cdc.gov/eid/article/14/10/08-0117_article |
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