Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum
Butanol is a promising alternative to fossil-derived fuels. Clostridium genus bacteria are known for their ability to produce butanol as one of the metabolites, however, at the moment this solution is not economically viable. To solve it, the process of butanol production should be optimized. While...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2024-01-01
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| Series: | Environmental and Climate Technologies |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/rtuect-2024-0007 |
| _version_ | 1797229521197334528 |
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| author | Feldmane Linda Raita Svetlana Berzina Indra Geiba Zane Mika Taras Kuzmika Iveta Spalvins Kriss |
| author_facet | Feldmane Linda Raita Svetlana Berzina Indra Geiba Zane Mika Taras Kuzmika Iveta Spalvins Kriss |
| author_sort | Feldmane Linda |
| collection | DOAJ |
| description | Butanol is a promising alternative to fossil-derived fuels. Clostridium genus bacteria are known for their ability to produce butanol as one of the metabolites, however, at the moment this solution is not economically viable. To solve it, the process of butanol production should be optimized. While ABE fermentation has been extensively studied, information about the optimal growth conditions for specific microorganisms often differs from one study to another. Therefore, this study aims to search for optimal growth conditions in sealed serum bottle tests for three widely used strains in ABE fermentation. In this study effects of temperature, pH, and agitation were tested on Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum. The optimal temperature for C. beijerinckii growth and butanol production was 32 °C, the optimal agitation speed for growth was 0 rpm, but for butanol production, it was 200 rpm. For C. saccharoperbutylacetonicum growth and butanol production pH 7.5, 30 °C temperature and an agitation rate of 100 rpm were optimal, however, this effect was slight. For C. acetobutylicum cultivation optimal temperature, pH, and agitation rate were respectively 37 °C, 6.5, and 200 rpm. |
| first_indexed | 2024-03-07T21:33:41Z |
| format | Article |
| id | doaj.art-72f11b4cf30d4d92bdecc2da98df082a |
| institution | Directory Open Access Journal |
| issn | 2255-8837 |
| language | English |
| last_indexed | 2024-04-24T15:13:55Z |
| publishDate | 2024-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Environmental and Climate Technologies |
| spelling | doaj.art-72f11b4cf30d4d92bdecc2da98df082a2024-04-02T09:29:41ZengSciendoEnvironmental and Climate Technologies2255-88372024-01-01281718310.2478/rtuect-2024-0007Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicumFeldmane Linda0Raita Svetlana1Berzina Indra2Geiba Zane3Mika Taras4Kuzmika Iveta5Spalvins Kriss6Institute of Energy Systems and Environment, Riga Technical University, 12-k1 Āzenes iela, Riga, LV-1048, LatviaInstitute of Energy Systems and Environment, Riga Technical University, 12-k1 Āzenes iela, Riga, LV-1048, LatviaInstitute of Energy Systems and Environment, Riga Technical University, 12-k1 Āzenes iela, Riga, LV-1048, LatviaInstitute of Energy Systems and Environment, Riga Technical University, 12-k1 Āzenes iela, Riga, LV-1048, LatviaInstitute of Energy Systems and Environment, Riga Technical University, 12-k1 Āzenes iela, Riga, LV-1048, LatviaInstitute of Energy Systems and Environment, Riga Technical University, 12-k1 Āzenes iela, Riga, LV-1048, LatviaInstitute of Energy Systems and Environment, Riga Technical University, 12-k1 Āzenes iela, Riga, LV-1048, LatviaButanol is a promising alternative to fossil-derived fuels. Clostridium genus bacteria are known for their ability to produce butanol as one of the metabolites, however, at the moment this solution is not economically viable. To solve it, the process of butanol production should be optimized. While ABE fermentation has been extensively studied, information about the optimal growth conditions for specific microorganisms often differs from one study to another. Therefore, this study aims to search for optimal growth conditions in sealed serum bottle tests for three widely used strains in ABE fermentation. In this study effects of temperature, pH, and agitation were tested on Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum. The optimal temperature for C. beijerinckii growth and butanol production was 32 °C, the optimal agitation speed for growth was 0 rpm, but for butanol production, it was 200 rpm. For C. saccharoperbutylacetonicum growth and butanol production pH 7.5, 30 °C temperature and an agitation rate of 100 rpm were optimal, however, this effect was slight. For C. acetobutylicum cultivation optimal temperature, pH, and agitation rate were respectively 37 °C, 6.5, and 200 rpm.https://doi.org/10.2478/rtuect-2024-0007abe fermentationanaerobic fermentationbiobutanolbutanolclostridiumexternal fermentation factorsibe fermentation |
| spellingShingle | Feldmane Linda Raita Svetlana Berzina Indra Geiba Zane Mika Taras Kuzmika Iveta Spalvins Kriss Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum Environmental and Climate Technologies abe fermentation anaerobic fermentation biobutanol butanol clostridium external fermentation factors ibe fermentation |
| title | Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum |
| title_full | Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum |
| title_fullStr | Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum |
| title_full_unstemmed | Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum |
| title_short | Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum |
| title_sort | effects of temperature ph and agitation on growth and butanol production of clostridium acetobutylicum clostridium beijerinckii and clostridium saccharoperbutylacetonicum |
| topic | abe fermentation anaerobic fermentation biobutanol butanol clostridium external fermentation factors ibe fermentation |
| url | https://doi.org/10.2478/rtuect-2024-0007 |
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