CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort Study
Glioblastoma is the most frequent and aggressive brain tumor in adults. This study aims to evaluate the expression and prognostic impact of CD99, a membrane glycoprotein involved in cellular migration and invasion. In a cohort of patients with glioblastoma treated with surgery, radiotherapy and temo...
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| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/13/7/597 |
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| author | Andrea Rocca Fabiola Giudici Carmine Antonio Donofrio Cristina Bottin Maurizio Pinamonti Benvenuto Ferrari Francesco Schettini Estela Pineda Stefano Panni Marika Cominetti Patrizia D’Auria Simonetta Bianchini Elena Varotti Marco Ungari Stefano Ciccarelli Marzia Filippini Sarah Brenna Valentina Fiori Tomas Di Mambro Angelo Sparti Mauro Magnani Fabrizio Zanconati Daniele Generali Antonio Fioravanti |
| author_facet | Andrea Rocca Fabiola Giudici Carmine Antonio Donofrio Cristina Bottin Maurizio Pinamonti Benvenuto Ferrari Francesco Schettini Estela Pineda Stefano Panni Marika Cominetti Patrizia D’Auria Simonetta Bianchini Elena Varotti Marco Ungari Stefano Ciccarelli Marzia Filippini Sarah Brenna Valentina Fiori Tomas Di Mambro Angelo Sparti Mauro Magnani Fabrizio Zanconati Daniele Generali Antonio Fioravanti |
| author_sort | Andrea Rocca |
| collection | DOAJ |
| description | Glioblastoma is the most frequent and aggressive brain tumor in adults. This study aims to evaluate the expression and prognostic impact of CD99, a membrane glycoprotein involved in cellular migration and invasion. In a cohort of patients with glioblastoma treated with surgery, radiotherapy and temozolomide, we retrospectively analyzed tumor expression of CD99 by immunohistochemistry (IHC) and by quantitative real-time polymerase chain reaction (qRT-PCR) for both the wild type (CD99wt) and the truncated (CD99sh) isoforms. The impact on overall survival (OS) was assessed with the Kaplan–Meier method and log-rank test and by multivariable Cox regression. Forty-six patients with glioblastoma entered this study. Immunohistochemical expression of CD99 was present in 83%. Only the CD99wt isoform was detected by qRT-PCR and was significantly correlated with CD99 expression evaluated by IHC (rho = 0.309, <i>p</i> = 0.037). CD99 expression was not associated with OS, regardless of the assessment methodology used (<i>p</i> = 0.61 for qRT-PCR and <i>p</i> = 0.73 for IHC). In an exploratory analysis of The Cancer Genome Atlas, casuistry of glioblastomas CD99 expression was not associated with OS nor with progression-free survival. This study confirms a high expression of CD99 in glioblastoma but does not show any significant impact on survival. Further preclinical studies are needed to define its role as a therapeutic target in glioblastoma. |
| first_indexed | 2024-04-24T10:47:50Z |
| format | Article |
| id | doaj.art-72f2663c2c414c968a37c62da85a97d3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-04-24T10:47:50Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-72f2663c2c414c968a37c62da85a97d32024-04-12T13:16:29ZengMDPI AGCells2073-44092024-03-0113759710.3390/cells13070597CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort StudyAndrea Rocca0Fabiola Giudici1Carmine Antonio Donofrio2Cristina Bottin3Maurizio Pinamonti4Benvenuto Ferrari5Francesco Schettini6Estela Pineda7Stefano Panni8Marika Cominetti9Patrizia D’Auria10Simonetta Bianchini11Elena Varotti12Marco Ungari13Stefano Ciccarelli14Marzia Filippini15Sarah Brenna16Valentina Fiori17Tomas Di Mambro18Angelo Sparti19Mauro Magnani20Fabrizio Zanconati21Daniele Generali22Antonio Fioravanti23Department of Medical, Surgical and Health Sciences, University of Trieste, 34147 Trieste, ItalyCancer Epidemiology Unit, Centro di Riferimento Oncologico di Aviano (CRO) IRCCS, 33081 Aviano, ItalyNeurosurgery, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyDepartment of Medical, Surgical and Health Sciences, University of Trieste, 34147 Trieste, ItalyDepartment of Medical, Surgical and Health Sciences, University of Trieste, 34147 Trieste, ItalyBreast and Brain Unit, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyTranslational Genomics and Targeted Therapies in Solid Tumors Group, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), C. Villaroel 170, 08036 Barcelona, SpainMedical Oncology Department, Hospital Clínic of Barcelona, 08036 Barcelona, SpainBreast and Brain Unit, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyNeurosurgery, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyNeurosurgery, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyNeurosurgery, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyPathology Unit, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyPathology Unit, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyRadiotherapy Unit, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyRadiotherapy Unit, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyRadiotherapy Unit, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyDiatheva srl, 61030 Cartoceto, ItalyDiatheva srl, 61030 Cartoceto, ItalyDepartment of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, ItalyDepartment of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, ItalyDepartment of Medical, Surgical and Health Sciences, University of Trieste, 34147 Trieste, ItalyDepartment of Medical, Surgical and Health Sciences, University of Trieste, 34147 Trieste, ItalyNeurosurgery, ASST Cremona, Viale Concordia 1, 26100 Cremona, ItalyGlioblastoma is the most frequent and aggressive brain tumor in adults. This study aims to evaluate the expression and prognostic impact of CD99, a membrane glycoprotein involved in cellular migration and invasion. In a cohort of patients with glioblastoma treated with surgery, radiotherapy and temozolomide, we retrospectively analyzed tumor expression of CD99 by immunohistochemistry (IHC) and by quantitative real-time polymerase chain reaction (qRT-PCR) for both the wild type (CD99wt) and the truncated (CD99sh) isoforms. The impact on overall survival (OS) was assessed with the Kaplan–Meier method and log-rank test and by multivariable Cox regression. Forty-six patients with glioblastoma entered this study. Immunohistochemical expression of CD99 was present in 83%. Only the CD99wt isoform was detected by qRT-PCR and was significantly correlated with CD99 expression evaluated by IHC (rho = 0.309, <i>p</i> = 0.037). CD99 expression was not associated with OS, regardless of the assessment methodology used (<i>p</i> = 0.61 for qRT-PCR and <i>p</i> = 0.73 for IHC). In an exploratory analysis of The Cancer Genome Atlas, casuistry of glioblastomas CD99 expression was not associated with OS nor with progression-free survival. This study confirms a high expression of CD99 in glioblastoma but does not show any significant impact on survival. Further preclinical studies are needed to define its role as a therapeutic target in glioblastoma.https://www.mdpi.com/2073-4409/13/7/597glioblastomaprognostic factorCD99quantitative real-time polymerase chain reactionimmunohistochemistry |
| spellingShingle | Andrea Rocca Fabiola Giudici Carmine Antonio Donofrio Cristina Bottin Maurizio Pinamonti Benvenuto Ferrari Francesco Schettini Estela Pineda Stefano Panni Marika Cominetti Patrizia D’Auria Simonetta Bianchini Elena Varotti Marco Ungari Stefano Ciccarelli Marzia Filippini Sarah Brenna Valentina Fiori Tomas Di Mambro Angelo Sparti Mauro Magnani Fabrizio Zanconati Daniele Generali Antonio Fioravanti CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort Study Cells glioblastoma prognostic factor CD99 quantitative real-time polymerase chain reaction immunohistochemistry |
| title | CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort Study |
| title_full | CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort Study |
| title_fullStr | CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort Study |
| title_full_unstemmed | CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort Study |
| title_short | CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort Study |
| title_sort | cd99 expression and prognostic impact in glioblastoma a single center cohort study |
| topic | glioblastoma prognostic factor CD99 quantitative real-time polymerase chain reaction immunohistochemistry |
| url | https://www.mdpi.com/2073-4409/13/7/597 |
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