IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS
We performed clinical observations and laboratory examination of 22 patients with chronic pyelonephritis (chronic renal failure, CRF) and 30 healthy individuals. The patients with CRF were examined twice. The first group (Group I) included patients with exacerbation of the disease. The comparison se...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2019-05-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/1475 |
| _version_ | 1797197951788908544 |
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| author | N. B. Zakharova L. Kh. Pastushkova R. V. Lakh I. M. Larina E. N. Nikolaev A. N. Ponukalin N. A. Varaksin V. I. Оficerov A. S. Kononikhin |
| author_facet | N. B. Zakharova L. Kh. Pastushkova R. V. Lakh I. M. Larina E. N. Nikolaev A. N. Ponukalin N. A. Varaksin V. I. Оficerov A. S. Kononikhin |
| author_sort | N. B. Zakharova |
| collection | DOAJ |
| description | We performed clinical observations and laboratory examination of 22 patients with chronic pyelonephritis (chronic renal failure, CRF) and 30 healthy individuals. The patients with CRF were examined twice. The first group (Group I) included patients with exacerbation of the disease. The comparison series (Group II) was represented by the same patients who were examined 1.5-3 months after completion of treatment, without clinical exacerbation of chronic pyelonephritis (CPN). Laboratory signs of acute renal damage were not detectable in all the patients examined. Concentrations of VEGF, MCP-1, IL-8 and IL-18 were determined in urine samples of all examined persons by ELISA technique. Protein spectrum of urine was assessed in six patients from Group I, and in six cases of Group II by means of mass spectrometry, using Agilent 1100 chromatographic device, and LTQ-FT Ultra hybrid mass spectrometer. The results of parallel determination of urine proteins by the two methods have shown that the evolving CPN exacerbation is associated with local secondary immune deficiency at the level of renal tubular urothelium. Determination of urine proteome by means of mass spectrometry in exacerbating disease allows identify the proteins associated with damage to epithelial lining of renal tubules and development of local immune response. |
| first_indexed | 2024-03-08T05:48:48Z |
| format | Article |
| id | doaj.art-72f4ea6ff7004177bd871d396de4536d |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:52:08Z |
| publishDate | 2019-05-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-72f4ea6ff7004177bd871d396de4536d2024-04-22T13:07:43ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2019-05-0121234135010.15789/1563-0625-2019-2-341-3501108IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODSN. B. Zakharova0L. Kh. Pastushkova1R. V. Lakh2I. M. Larina3E. N. Nikolaev4A. N. Ponukalin5N. A. Varaksin6V. I. Оficerov7A. S. Kononikhin8Saratov State Medical University n.a. V.I. RazumovskyInstitute of Biomedical Problems RASSaratov State Medical University n.a. V.I. RazumovskyInstitute of Biomedical Problems RASV.L. Talrose Institute of Energy Problems of Chemical Physics, Russian Academy of SciencesSaratov State Medical University n.a. V.I. RazumovskyAO «Vector Best»AO «Vector Best»V.L. Talrose Institute of Energy Problems of Chemical Physics, Russian Academy of SciencesWe performed clinical observations and laboratory examination of 22 patients with chronic pyelonephritis (chronic renal failure, CRF) and 30 healthy individuals. The patients with CRF were examined twice. The first group (Group I) included patients with exacerbation of the disease. The comparison series (Group II) was represented by the same patients who were examined 1.5-3 months after completion of treatment, without clinical exacerbation of chronic pyelonephritis (CPN). Laboratory signs of acute renal damage were not detectable in all the patients examined. Concentrations of VEGF, MCP-1, IL-8 and IL-18 were determined in urine samples of all examined persons by ELISA technique. Protein spectrum of urine was assessed in six patients from Group I, and in six cases of Group II by means of mass spectrometry, using Agilent 1100 chromatographic device, and LTQ-FT Ultra hybrid mass spectrometer. The results of parallel determination of urine proteins by the two methods have shown that the evolving CPN exacerbation is associated with local secondary immune deficiency at the level of renal tubular urothelium. Determination of urine proteome by means of mass spectrometry in exacerbating disease allows identify the proteins associated with damage to epithelial lining of renal tubules and development of local immune response.https://www.mimmun.ru/mimmun/article/view/1475pyelonephritiscytokinesurinary biomarkersproteomicsmass spectrometry |
| spellingShingle | N. B. Zakharova L. Kh. Pastushkova R. V. Lakh I. M. Larina E. N. Nikolaev A. N. Ponukalin N. A. Varaksin V. I. Оficerov A. S. Kononikhin IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS Медицинская иммунология pyelonephritis cytokines urinary biomarkers proteomics mass spectrometry |
| title | IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS |
| title_full | IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS |
| title_fullStr | IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS |
| title_full_unstemmed | IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS |
| title_short | IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS |
| title_sort | identification of biomarkers of renal parenchyma damage in the urine of patients with chronic pyelonephritis by elisa and mass spectrometry methods |
| topic | pyelonephritis cytokines urinary biomarkers proteomics mass spectrometry |
| url | https://www.mimmun.ru/mimmun/article/view/1475 |
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