Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood
Seabirds are widely regarded as an invaluable bioindicator of environmental health. Matrices including eggs and feathers have been used as non-lethal means to assess contaminant burdens. We have developed a new approach for extraction of polycyclic aromatic compounds (PACs) from seabird plasma and s...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2297-8739/10/1/48 |
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| author | Vida Moradi Thor Halldorson Ifeoluwa Idowu Zhe Xia Nipuni Vitharana Chris Marvin Philippe J. Thomas Gregg T. Tomy |
| author_facet | Vida Moradi Thor Halldorson Ifeoluwa Idowu Zhe Xia Nipuni Vitharana Chris Marvin Philippe J. Thomas Gregg T. Tomy |
| author_sort | Vida Moradi |
| collection | DOAJ |
| description | Seabirds are widely regarded as an invaluable bioindicator of environmental health. Matrices including eggs and feathers have been used as non-lethal means to assess contaminant burdens. We have developed a new approach for extraction of polycyclic aromatic compounds (PACs) from seabird plasma and serum based on automated microbead-beating homogenization and extraction. Commercially available bovine serum and plasma were purposely fortified with a suite of PACs separately at three dosing levels, placed inside a custom-made stainless-steel tube containing ceramic microbeads, and subjected to an extraction process using a Precellys tissue homogenizer. Tubes were shaken forcefully in three-dimensions, facilitating high mass-transfer of PACs from the matrix into the hexane extraction solvent. The accuracy of the method ranged from 55 to 120% and limits of detection and quantitation ranged from 0.1 to 8 and 0.2 to 27 pg/μL, respectively. The method exhibited good repeatability with both inter- and intra-day repeatability < 30%. The developed method represents an effective and efficient approach to extraction of PACs from important biological matrices. |
| first_indexed | 2024-03-09T11:14:59Z |
| format | Article |
| id | doaj.art-72f62ff99b0f4ca88317993469ccb5d7 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T11:14:59Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-72f62ff99b0f4ca88317993469ccb5d72023-12-01T00:32:47ZengMDPI AGSeparations2297-87392023-01-011014810.3390/separations10010048Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole BloodVida Moradi0Thor Halldorson1Ifeoluwa Idowu2Zhe Xia3Nipuni Vitharana4Chris Marvin5Philippe J. Thomas6Gregg T. Tomy7Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaWater Science and Technology Directorate, Environment and Climate Change Canada, Burlington, ON L7S 1A1, CanadaWildlife Landscape Science Directorate, Environment and Climate Change Canada, Ottawa, ON K1A 0H3, CanadaDepartment of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaSeabirds are widely regarded as an invaluable bioindicator of environmental health. Matrices including eggs and feathers have been used as non-lethal means to assess contaminant burdens. We have developed a new approach for extraction of polycyclic aromatic compounds (PACs) from seabird plasma and serum based on automated microbead-beating homogenization and extraction. Commercially available bovine serum and plasma were purposely fortified with a suite of PACs separately at three dosing levels, placed inside a custom-made stainless-steel tube containing ceramic microbeads, and subjected to an extraction process using a Precellys tissue homogenizer. Tubes were shaken forcefully in three-dimensions, facilitating high mass-transfer of PACs from the matrix into the hexane extraction solvent. The accuracy of the method ranged from 55 to 120% and limits of detection and quantitation ranged from 0.1 to 8 and 0.2 to 27 pg/μL, respectively. The method exhibited good repeatability with both inter- and intra-day repeatability < 30%. The developed method represents an effective and efficient approach to extraction of PACs from important biological matrices.https://www.mdpi.com/2297-8739/10/1/48polycyclic aromatic compoundsplasmaserumseabirdsmicrobead-beating extraction |
| spellingShingle | Vida Moradi Thor Halldorson Ifeoluwa Idowu Zhe Xia Nipuni Vitharana Chris Marvin Philippe J. Thomas Gregg T. Tomy Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood Separations polycyclic aromatic compounds plasma serum seabirds microbead-beating extraction |
| title | Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood |
| title_full | Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood |
| title_fullStr | Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood |
| title_full_unstemmed | Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood |
| title_short | Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood |
| title_sort | microbead beating extraction of polycyclic aromatic compounds from seabird plasma and whole blood |
| topic | polycyclic aromatic compounds plasma serum seabirds microbead-beating extraction |
| url | https://www.mdpi.com/2297-8739/10/1/48 |
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