Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media
Multipotent porcine mesenchymal stem cells (pMSC) are invaluable for research and therapeutic use in regenerative medicine. Media used for derivation and expansion of pMSC may play an important role for the selection of MSC subpopulation at an early stage and thereby, the specific basal medium may a...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2020-10-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/9/10/bio053280 |
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| author | Sangeetha Kannan Jyotirmoy Ghosh Sujoy K. Dhara |
| author_facet | Sangeetha Kannan Jyotirmoy Ghosh Sujoy K. Dhara |
| author_sort | Sangeetha Kannan |
| collection | DOAJ |
| description | Multipotent porcine mesenchymal stem cells (pMSC) are invaluable for research and therapeutic use in regenerative medicine. Media used for derivation and expansion of pMSC may play an important role for the selection of MSC subpopulation at an early stage and thereby, the specific basal medium may also affect differentiation potential of these cells. The present study was undertaken to evaluate the effects of αMEM, aDMEM, M199, αMEM/M199, aDMEM/M199 and αMEM/aDMEM media on (1) porcine bone marrow MSC derivation; (2) expression of number of osteogenic markers (ALP, COL1A1, SPP1 and BGLAP) at 5th and 10th passage in pMSC before differentiation; and (3) differentiation of pMSC (at 5th passage) to osteogenic lineage. Morphological changes and matrix formation in osteogenic cells were evaluated by microscopic examination. Calcium deposits in osteocytes were confirmed by Alizarin Red S staining. Based on expression of different markers, it was evident that selection of bone marrow pMSC subpopulations was independent of basal media used. However, the differentiation of those pMSCs, specifically to osteogenic lineage, was dependent on the medium used for expansion of pMSC at the pre-differentiation stage. We demonstrated here that the pMSC grown in combined αMEM/aDMEM (1:1) medium expressed number of osteogenic markers and these pMSC underwent osteogenic differentiation most efficiently, in comparison to porcine mesenchymal stem cells grown in other media. In conclusion, osteogenic differentiation potential of pMSC maintained in αMEM/aDMEM medium was observed significantly higher compared to cells cultivated in other media and therefore, the combined medium αMEM/aDMEM (1:1) may preferentially be used for expansion of pMSC, if needed for osteogenic differentiation. |
| first_indexed | 2024-12-21T17:04:11Z |
| format | Article |
| id | doaj.art-72f6d55996214bd699d0943c1ae306f7 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-21T17:04:11Z |
| publishDate | 2020-10-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-72f6d55996214bd699d0943c1ae306f72022-12-21T18:56:34ZengThe Company of BiologistsBiology Open2046-63902020-10-0191010.1242/bio.053280053280Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal mediaSangeetha Kannan0Jyotirmoy Ghosh1Sujoy K. Dhara2 Department of Biotechnology, Jain University, Bangalore 560011, Karnataka, India Molecular Biology Laboratory, ICAR-National Institute of Animal Nutrition and Physiology, Bangalore 560030, Karnataka, India Stem Cell Laboratory, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India Multipotent porcine mesenchymal stem cells (pMSC) are invaluable for research and therapeutic use in regenerative medicine. Media used for derivation and expansion of pMSC may play an important role for the selection of MSC subpopulation at an early stage and thereby, the specific basal medium may also affect differentiation potential of these cells. The present study was undertaken to evaluate the effects of αMEM, aDMEM, M199, αMEM/M199, aDMEM/M199 and αMEM/aDMEM media on (1) porcine bone marrow MSC derivation; (2) expression of number of osteogenic markers (ALP, COL1A1, SPP1 and BGLAP) at 5th and 10th passage in pMSC before differentiation; and (3) differentiation of pMSC (at 5th passage) to osteogenic lineage. Morphological changes and matrix formation in osteogenic cells were evaluated by microscopic examination. Calcium deposits in osteocytes were confirmed by Alizarin Red S staining. Based on expression of different markers, it was evident that selection of bone marrow pMSC subpopulations was independent of basal media used. However, the differentiation of those pMSCs, specifically to osteogenic lineage, was dependent on the medium used for expansion of pMSC at the pre-differentiation stage. We demonstrated here that the pMSC grown in combined αMEM/aDMEM (1:1) medium expressed number of osteogenic markers and these pMSC underwent osteogenic differentiation most efficiently, in comparison to porcine mesenchymal stem cells grown in other media. In conclusion, osteogenic differentiation potential of pMSC maintained in αMEM/aDMEM medium was observed significantly higher compared to cells cultivated in other media and therefore, the combined medium αMEM/aDMEM (1:1) may preferentially be used for expansion of pMSC, if needed for osteogenic differentiation.http://bio.biologists.org/content/9/10/bio053280bone marrowbasal mediamesenchymal stem cellsosteogenic differentiationporcine |
| spellingShingle | Sangeetha Kannan Jyotirmoy Ghosh Sujoy K. Dhara Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media Biology Open bone marrow basal media mesenchymal stem cells osteogenic differentiation porcine |
| title | Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media |
| title_full | Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media |
| title_fullStr | Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media |
| title_full_unstemmed | Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media |
| title_short | Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media |
| title_sort | osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media |
| topic | bone marrow basal media mesenchymal stem cells osteogenic differentiation porcine |
| url | http://bio.biologists.org/content/9/10/bio053280 |
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