Clinicopathologic features and KRAS mutation incidence of gastric carcinomas
Objectives: This study aims to determine the frequency of KRAS mutations in patients with gastric adenocarcinoma (GAC) in Hatay province, and to determine the relationship of this mutation with some pathological and clinical parameters and to guide the diagnosis and treatment planning of patients....
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| Format: | Article |
| Language: | English |
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Rabia Yılmaz
2022-01-01
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| Series: | Journal of Contemporary Medicine |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1912312 |
| _version_ | 1827980131007725568 |
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| author | Didar Gürsoy İlke Evrim Seçinti Esin Doğan Muhyittin Temiz |
| author_facet | Didar Gürsoy İlke Evrim Seçinti Esin Doğan Muhyittin Temiz |
| author_sort | Didar Gürsoy |
| collection | DOAJ |
| description | Objectives: This study aims to determine the frequency of KRAS mutations in patients with gastric adenocarcinoma (GAC) in Hatay province, and to determine the relationship of this mutation with some pathological and clinical parameters and to guide the diagnosis and treatment planning of patients.
Methods: Formalin-fixed, paraffin-embedded, and histologically confirmed samples were used in the assessment of KRAS mutation. Sections were taken from the archive tissue samples of each case. Real-Time Polymerase Chain Reaction (RT-PCR) system was used to identify mutations of codons 12 and 13 (exon 2) of the RAS gene. Mutations of GLY12ALA (G12A), GLY12ASP (G12D), GLY12ARG (G12R), GLY12CYS (G12C), GLY12SER (G12S), GLY12VAL (G12V), GLY13ASP (G13D) were performed.
Results: The mutation rate of KRAS was 2% and only one substitution, G12D, was detected. In this case, the tumor was located in the small curvature. Since the number of cases with mutations was low, statistical comparison could not be made between KRAS mutation and clinicopathological factors. A significant difference was found between tumor differentiation and WHO-2010 typing and primary tumor stage.
Conclusions: We found the incidence of KRAS mutation to be 2%. We also estimate that the G12D mutation may be associated with GAC site and surgical margin. Although KRAS mutation in GAC alone is not a prognostic or predictive marker, subtype-specific analysis may provide data that may affect the diagnosis, management and treatment of the disease |
| first_indexed | 2024-04-09T21:48:50Z |
| format | Article |
| id | doaj.art-72f7aa8a474b453c9452d2c21c6dbca4 |
| institution | Directory Open Access Journal |
| issn | 2667-7180 |
| language | English |
| last_indexed | 2024-04-09T21:48:50Z |
| publishDate | 2022-01-01 |
| publisher | Rabia Yılmaz |
| record_format | Article |
| series | Journal of Contemporary Medicine |
| spelling | doaj.art-72f7aa8a474b453c9452d2c21c6dbca42023-03-24T19:43:26ZengRabia YılmazJournal of Contemporary Medicine2667-71802022-01-011211510.16899/jcm.9796651809Clinicopathologic features and KRAS mutation incidence of gastric carcinomasDidar Gürsoy0İlke Evrim Seçinti1Esin Doğan2Muhyittin Temiz3HATAY MUSTAFA KEMAL ÜNİVERSİTESİ, TAYFUR ATA SÖKMEN TIP FAKÜLTESİ, CERRAHİ TIP BİLİMLERİ BÖLÜMÜ, PATOLOJİ ANABİLİM DALIHATAY MUSTAFA KEMAL ÜNİVERSİTESİ, TAYFUR ATA SÖKMEN TIP FAKÜLTESİ, CERRAHİ TIP BİLİMLERİ BÖLÜMÜ, PATOLOJİ ANABİLİM DALIHATAY MUSTAFA KEMAL ÜNİVERSİTESİ, TAYFUR ATA SÖKMEN TIP FAKÜLTESİ, CERRAHİ TIP BİLİMLERİ BÖLÜMÜ, PATOLOJİ ANABİLİM DALIHATAY MUSTAFA KEMAL ÜNİVERSİTESİ, TAYFUR ATA SÖKMEN TIP FAKÜLTESİ, CERRAHİ TIP BİLİMLERİ BÖLÜMÜ, GENEL CERRAHİ ANABİLİM DALIObjectives: This study aims to determine the frequency of KRAS mutations in patients with gastric adenocarcinoma (GAC) in Hatay province, and to determine the relationship of this mutation with some pathological and clinical parameters and to guide the diagnosis and treatment planning of patients. Methods: Formalin-fixed, paraffin-embedded, and histologically confirmed samples were used in the assessment of KRAS mutation. Sections were taken from the archive tissue samples of each case. Real-Time Polymerase Chain Reaction (RT-PCR) system was used to identify mutations of codons 12 and 13 (exon 2) of the RAS gene. Mutations of GLY12ALA (G12A), GLY12ASP (G12D), GLY12ARG (G12R), GLY12CYS (G12C), GLY12SER (G12S), GLY12VAL (G12V), GLY13ASP (G13D) were performed. Results: The mutation rate of KRAS was 2% and only one substitution, G12D, was detected. In this case, the tumor was located in the small curvature. Since the number of cases with mutations was low, statistical comparison could not be made between KRAS mutation and clinicopathological factors. A significant difference was found between tumor differentiation and WHO-2010 typing and primary tumor stage. Conclusions: We found the incidence of KRAS mutation to be 2%. We also estimate that the G12D mutation may be associated with GAC site and surgical margin. Although KRAS mutation in GAC alone is not a prognostic or predictive marker, subtype-specific analysis may provide data that may affect the diagnosis, management and treatment of the diseasehttps://dergipark.org.tr/tr/download/article-file/1912312krasmide kanserig12dmutasyonkrasgastric carcinomag12dmutation |
| spellingShingle | Didar Gürsoy İlke Evrim Seçinti Esin Doğan Muhyittin Temiz Clinicopathologic features and KRAS mutation incidence of gastric carcinomas Journal of Contemporary Medicine kras mide kanseri g12d mutasyon kras gastric carcinoma g12d mutation |
| title | Clinicopathologic features and KRAS mutation incidence of gastric carcinomas |
| title_full | Clinicopathologic features and KRAS mutation incidence of gastric carcinomas |
| title_fullStr | Clinicopathologic features and KRAS mutation incidence of gastric carcinomas |
| title_full_unstemmed | Clinicopathologic features and KRAS mutation incidence of gastric carcinomas |
| title_short | Clinicopathologic features and KRAS mutation incidence of gastric carcinomas |
| title_sort | clinicopathologic features and kras mutation incidence of gastric carcinomas |
| topic | kras mide kanseri g12d mutasyon kras gastric carcinoma g12d mutation |
| url | https://dergipark.org.tr/tr/download/article-file/1912312 |
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