Potential Synergy between Spores of <i>Metarhizium anisopliae</i> and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development
Date palm dust mites are important pests severely infesting valuable nutritious fruits (dates) of date palm. In search of an alternative to acaricides, joint action of <i>Metarhizium anisopliae</i> EBCL 02049 spores and 1-Chlorooctadecane was evaluated as a potential candidate for the ma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/8/1900 |
| _version_ | 1797570184972599296 |
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| author | Abid Hussain Ahmed Mohammed AlJabr |
| author_facet | Abid Hussain Ahmed Mohammed AlJabr |
| author_sort | Abid Hussain |
| collection | DOAJ |
| description | Date palm dust mites are important pests severely infesting valuable nutritious fruits (dates) of date palm. In search of an alternative to acaricides, joint action of <i>Metarhizium anisopliae</i> EBCL 02049 spores and 1-Chlorooctadecane was evaluated as a potential candidate for the management of <i>Oligonychus afrasiaticus</i> through natural products. In this regard, in vitro tests were performed to evaluate the interaction of <i>M. anisopliae</i> spores with multiple doses of 1-Chlorooctadecane (0.8, 1.6, 2.4, 3.2, and 4.0 mg/mL). Compatibility bioassay results evidenced from vegetative growth (77.7–84.40 mm), sporulation (5.50–7.30 × 10<sup>6</sup> spores/mL), and germination (96.70–98.20%), revealed that all the tested doses are compatible (biological index > 82) with the spores of <i>M. anisopliae</i>. The impact of combined treatment of spores with 1-Chlorooctadecane in different proportions (Scheme I, II, III, and IV) compared to their sole application against <i>O. afrasiaticus</i> was evaluated by concentration–mortality response bioassays. Results showed that all the combined treatments revealed high mortality compared to the sole application, which showed relatively slow mortality response over time. Toxicity recorded from Scheme IV combinations (80% 1-Chlorooctadecane: 20% Spores), exhibited strong synergistic interaction (joint toxicity = 713). Furthermore, potent interactions have overcome the host antioxidant defense at the final stage of infection by tremendously reducing catalase, and superoxide dismutase activities. These experiments demonstrated fungal–toxin joint synergistic interaction as a promising date palm dust mite management option. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:21:20Z |
| publishDate | 2020-04-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-72f7ebf294184090b50ce8b96decb1a02023-11-19T22:10:31ZengMDPI AGMolecules1420-30492020-04-01258190010.3390/molecules25081900Potential Synergy between Spores of <i>Metarhizium anisopliae</i> and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide DevelopmentAbid Hussain0Ahmed Mohammed AlJabr1Laboratory of Bio-Control and Molecular Biology, Department of Arid Land Agriculture, College of Agricultural and Food Sciences, King Faisal University, Hofuf 31982, Al-Ahsa, Saudi ArabiaLaboratory of Bio-Control and Molecular Biology, Department of Arid Land Agriculture, College of Agricultural and Food Sciences, King Faisal University, Hofuf 31982, Al-Ahsa, Saudi ArabiaDate palm dust mites are important pests severely infesting valuable nutritious fruits (dates) of date palm. In search of an alternative to acaricides, joint action of <i>Metarhizium anisopliae</i> EBCL 02049 spores and 1-Chlorooctadecane was evaluated as a potential candidate for the management of <i>Oligonychus afrasiaticus</i> through natural products. In this regard, in vitro tests were performed to evaluate the interaction of <i>M. anisopliae</i> spores with multiple doses of 1-Chlorooctadecane (0.8, 1.6, 2.4, 3.2, and 4.0 mg/mL). Compatibility bioassay results evidenced from vegetative growth (77.7–84.40 mm), sporulation (5.50–7.30 × 10<sup>6</sup> spores/mL), and germination (96.70–98.20%), revealed that all the tested doses are compatible (biological index > 82) with the spores of <i>M. anisopliae</i>. The impact of combined treatment of spores with 1-Chlorooctadecane in different proportions (Scheme I, II, III, and IV) compared to their sole application against <i>O. afrasiaticus</i> was evaluated by concentration–mortality response bioassays. Results showed that all the combined treatments revealed high mortality compared to the sole application, which showed relatively slow mortality response over time. Toxicity recorded from Scheme IV combinations (80% 1-Chlorooctadecane: 20% Spores), exhibited strong synergistic interaction (joint toxicity = 713). Furthermore, potent interactions have overcome the host antioxidant defense at the final stage of infection by tremendously reducing catalase, and superoxide dismutase activities. These experiments demonstrated fungal–toxin joint synergistic interaction as a promising date palm dust mite management option.https://www.mdpi.com/1420-3049/25/8/1900antioxidantscompatibilitydate palm dust miteshost defense<i>Oligonychus afrasiaticus</i><i>Metarhizium anisopliae</i> |
| spellingShingle | Abid Hussain Ahmed Mohammed AlJabr Potential Synergy between Spores of <i>Metarhizium anisopliae</i> and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development Molecules antioxidants compatibility date palm dust mites host defense <i>Oligonychus afrasiaticus</i> <i>Metarhizium anisopliae</i> |
| title | Potential Synergy between Spores of <i>Metarhizium anisopliae</i> and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development |
| title_full | Potential Synergy between Spores of <i>Metarhizium anisopliae</i> and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development |
| title_fullStr | Potential Synergy between Spores of <i>Metarhizium anisopliae</i> and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development |
| title_full_unstemmed | Potential Synergy between Spores of <i>Metarhizium anisopliae</i> and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development |
| title_short | Potential Synergy between Spores of <i>Metarhizium anisopliae</i> and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development |
| title_sort | potential synergy between spores of i metarhizium anisopliae i and plant secondary metabolite 1 chlorooctadecane for effective natural acaricide development |
| topic | antioxidants compatibility date palm dust mites host defense <i>Oligonychus afrasiaticus</i> <i>Metarhizium anisopliae</i> |
| url | https://www.mdpi.com/1420-3049/25/8/1900 |
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