Analgesic Effects Elicited by Neuroactive Mediators Injected into the ST 36 Acupuncture Point on Inflammatory and Neuropathic Pain in Mice
The present study evaluates whether the injection of serotonin, acetylcholine, glutamate, bradykinin, histamine, or substance P (SP) into the Zusanli (Stomach 36, ST 36) acupoint can also produce the acupuncture-induced antinociceptive effect on inflammatory or neuropathic pain. In this in vivo expe...
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| Format: | Article |
| Language: | English |
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Medical Association of Pharmacopuncture Institute
2018-10-01
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| Series: | Journal of Acupuncture & Meridian Studies |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2005290118300013 |
| _version_ | 1818648375900241920 |
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| author | Jádina S. Vieira Jéssica A. Toreti Ravena C. de Carvalho João E. de Araújo Marcelo L. Silva Josie R.T. Silva |
| author_facet | Jádina S. Vieira Jéssica A. Toreti Ravena C. de Carvalho João E. de Araújo Marcelo L. Silva Josie R.T. Silva |
| author_sort | Jádina S. Vieira |
| collection | DOAJ |
| description | The present study evaluates whether the injection of serotonin, acetylcholine, glutamate, bradykinin, histamine, or substance P (SP) into the Zusanli (Stomach 36, ST 36) acupoint can also produce the acupuncture-induced antinociceptive effect on inflammatory or neuropathic pain. In this in vivo experimental study, a total of 450 male Swiss mice were used. Mice were injected with saline or complete Freund's adjuvant (CFA) or subjected to sham or chronic constriction injury (CCI) surgery. After the establishment of the inflammatory (4 hours) or the neuropathic pain (3 days), the animals (n = 6) received manual acupuncture, sham acupuncture, or injection of saline, serotonin, acetylcholine, glutamate, bradykinin, histamine, or SP into the ST 36 and were evaluated for up to 24 hours. Mechanical threshold was evaluated, and the L4-L6 dorsal root ganglion was used for analysis of the transient receptor potential vanilloid type 1 overexpression. The mice from both the CFA and CCI models treated with manual acupuncture had significant increases in the thresholds for more than 24 hours. Sham acupuncture stimulation did not change the thresholds. In the mice injected with each of the mediators, the thresholds were significantly increased for all times in both the CFA and CCI models. Transient receptor potential vanilloid type 1 overexpression in CFA and CCI mice was reduced at all times by injection of serotonin, acetylcholine, or SP but not by injection of glutamate, histamine, or bradykinin. Our data suggest that the neuroactive mediators released by acupuncture-induced tissue injury may contribute to acupuncture-induced analgesia. Keywords: acupuncture-induced analgesia, inflammatory pain, neuroactive mediators, neuropathic pain |
| first_indexed | 2024-12-17T01:17:26Z |
| format | Article |
| id | doaj.art-72fb1a130f0243b1a3da74427d7d1099 |
| institution | Directory Open Access Journal |
| issn | 2005-2901 |
| language | English |
| last_indexed | 2024-12-17T01:17:26Z |
| publishDate | 2018-10-01 |
| publisher | Medical Association of Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Acupuncture & Meridian Studies |
| spelling | doaj.art-72fb1a130f0243b1a3da74427d7d10992022-12-21T22:08:56ZengMedical Association of Pharmacopuncture InstituteJournal of Acupuncture & Meridian Studies2005-29012018-10-01115280289Analgesic Effects Elicited by Neuroactive Mediators Injected into the ST 36 Acupuncture Point on Inflammatory and Neuropathic Pain in MiceJádina S. Vieira0Jéssica A. Toreti1Ravena C. de Carvalho2João E. de Araújo3Marcelo L. Silva4Josie R.T. Silva5Department of Physiotherapy, College of Nursing of the Federal University of Alfenas-UNIFAL, Alfenas, BrazilDepartment of Physiotherapy, College of Nursing of the Federal University of Alfenas-UNIFAL, Alfenas, BrazilDepartment of Physiotherapy, College of Nursing of the Federal University of Alfenas-UNIFAL, Alfenas, BrazilLaboratory of Neuropsychobiology and Motor Behavior, Department of Biomechanics, Medicine, and Rehabilitation of the Locomotor System, School of Medicine, University of São Paulo, Ribeirão Preto, BrazilDepartment of Physiotherapy, College of Nursing of the Federal University of Alfenas-UNIFAL, Alfenas, BrazilDepartment of Physiotherapy, College of Nursing of the Federal University of Alfenas-UNIFAL, Alfenas, Brazil; Corresponding author. Department of Physiotherapy, College of Nursing of the Federal University of Alfenas-UNIFAL, Avenue Jovino Fernandes Sales 2600, CEP 37130-000, Alfenas, MG, Brazil.The present study evaluates whether the injection of serotonin, acetylcholine, glutamate, bradykinin, histamine, or substance P (SP) into the Zusanli (Stomach 36, ST 36) acupoint can also produce the acupuncture-induced antinociceptive effect on inflammatory or neuropathic pain. In this in vivo experimental study, a total of 450 male Swiss mice were used. Mice were injected with saline or complete Freund's adjuvant (CFA) or subjected to sham or chronic constriction injury (CCI) surgery. After the establishment of the inflammatory (4 hours) or the neuropathic pain (3 days), the animals (n = 6) received manual acupuncture, sham acupuncture, or injection of saline, serotonin, acetylcholine, glutamate, bradykinin, histamine, or SP into the ST 36 and were evaluated for up to 24 hours. Mechanical threshold was evaluated, and the L4-L6 dorsal root ganglion was used for analysis of the transient receptor potential vanilloid type 1 overexpression. The mice from both the CFA and CCI models treated with manual acupuncture had significant increases in the thresholds for more than 24 hours. Sham acupuncture stimulation did not change the thresholds. In the mice injected with each of the mediators, the thresholds were significantly increased for all times in both the CFA and CCI models. Transient receptor potential vanilloid type 1 overexpression in CFA and CCI mice was reduced at all times by injection of serotonin, acetylcholine, or SP but not by injection of glutamate, histamine, or bradykinin. Our data suggest that the neuroactive mediators released by acupuncture-induced tissue injury may contribute to acupuncture-induced analgesia. Keywords: acupuncture-induced analgesia, inflammatory pain, neuroactive mediators, neuropathic painhttp://www.sciencedirect.com/science/article/pii/S2005290118300013 |
| spellingShingle | Jádina S. Vieira Jéssica A. Toreti Ravena C. de Carvalho João E. de Araújo Marcelo L. Silva Josie R.T. Silva Analgesic Effects Elicited by Neuroactive Mediators Injected into the ST 36 Acupuncture Point on Inflammatory and Neuropathic Pain in Mice Journal of Acupuncture & Meridian Studies |
| title | Analgesic Effects Elicited by Neuroactive Mediators Injected into the ST 36 Acupuncture Point on Inflammatory and Neuropathic Pain in Mice |
| title_full | Analgesic Effects Elicited by Neuroactive Mediators Injected into the ST 36 Acupuncture Point on Inflammatory and Neuropathic Pain in Mice |
| title_fullStr | Analgesic Effects Elicited by Neuroactive Mediators Injected into the ST 36 Acupuncture Point on Inflammatory and Neuropathic Pain in Mice |
| title_full_unstemmed | Analgesic Effects Elicited by Neuroactive Mediators Injected into the ST 36 Acupuncture Point on Inflammatory and Neuropathic Pain in Mice |
| title_short | Analgesic Effects Elicited by Neuroactive Mediators Injected into the ST 36 Acupuncture Point on Inflammatory and Neuropathic Pain in Mice |
| title_sort | analgesic effects elicited by neuroactive mediators injected into the st 36 acupuncture point on inflammatory and neuropathic pain in mice |
| url | http://www.sciencedirect.com/science/article/pii/S2005290118300013 |
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