Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes
Strawberry fruit Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), rich in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin enriched fraction of Romina st...
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| Format: | Article |
| Language: | srp |
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Pharmaceutical Association of Serbia, Belgrade, Serbia
2021-01-01
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| Series: | Arhiv za farmaciju |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2021/0004-19632103197S.pdf |
| _version_ | 1819151628548177920 |
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| author | Spremo-Potparević Biljana Čabarkapa-Pirković Andrea Dekanski Dragana Topalović Dijana Bajić Vladan Živković Lada |
| author_facet | Spremo-Potparević Biljana Čabarkapa-Pirković Andrea Dekanski Dragana Topalović Dijana Bajić Vladan Živković Lada |
| author_sort | Spremo-Potparević Biljana |
| collection | DOAJ |
| description | Strawberry fruit Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), rich in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxide in vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 µg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposure of the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the posttreatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 µg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent. |
| first_indexed | 2024-12-22T14:36:25Z |
| format | Article |
| id | doaj.art-72fc6482a1e74db8bbf3e7334a47c879 |
| institution | Directory Open Access Journal |
| issn | 0004-1963 2217-8767 |
| language | srp |
| last_indexed | 2024-12-22T14:36:25Z |
| publishDate | 2021-01-01 |
| publisher | Pharmaceutical Association of Serbia, Belgrade, Serbia |
| record_format | Article |
| series | Arhiv za farmaciju |
| spelling | doaj.art-72fc6482a1e74db8bbf3e7334a47c8792022-12-21T18:22:38ZsrpPharmaceutical Association of Serbia, Belgrade, SerbiaArhiv za farmaciju0004-19632217-87672021-01-0171319720610.5937/arhfarm71-310600004-19632103197SAntigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytesSpremo-Potparević Biljana0https://orcid.org/0000-0001-5570-883XČabarkapa-Pirković Andrea1Dekanski Dragana2https://orcid.org/0000-0001-8467-4165Topalović Dijana3https://orcid.org/0000-0001-6546-2626Bajić Vladan4https://orcid.org/0000-0002-2333-8245Živković Lada5https://orcid.org/0000-0002-8087-1927University of Belgrade, Faculty of Pharmacy, Center of Biological Research, Belgrade, SerbiaUniversity of Belgrade, Faculty of Pharmacy, Center of Biological Research, Belgrade, SerbiaUniversity of Belgrade, Institute for Application of Nuclear Energy-INEP, Department for Biology of Reproduction, Zemun, SerbiaUniversity of Belgrade, Faculty of Pharmacy, Center of Biological Research, Belgrade, SerbiaUniversity of Belgrade, Institute of Nuclear Sciences Vinča, Laboratory for Radiobiology and Molecular Genetics, Belgrade, SerbiaUniversity of Belgrade, Faculty of Pharmacy, Center of Biological Research, Belgrade, SerbiaStrawberry fruit Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), rich in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxide in vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 µg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposure of the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the posttreatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 µg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent.https://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2021/0004-19632103197S.pdfdna damagecomet assaystrawberry (cv. romina)anthocyaninsleukocytes |
| spellingShingle | Spremo-Potparević Biljana Čabarkapa-Pirković Andrea Dekanski Dragana Topalović Dijana Bajić Vladan Živković Lada Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes Arhiv za farmaciju dna damage comet assay strawberry (cv. romina) anthocyanins leukocytes |
| title | Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes |
| title_full | Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes |
| title_fullStr | Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes |
| title_full_unstemmed | Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes |
| title_short | Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes |
| title_sort | antigenotoxic properties of anthocyanin enriched fraction of strawberry cv romina extract on dna damage induced by h2o2 in human peripheral blood leukocytes |
| topic | dna damage comet assay strawberry (cv. romina) anthocyanins leukocytes |
| url | https://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2021/0004-19632103197S.pdf |
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