Determination of Praziquantel in <i>Sparus aurata</i> L. after Administration of Medicated Animal Feed
Praziquantel (PZQ) is an anthelmintic drug used in humans and animals against Platyhelminthes and in aquaculture in the Far East. Medicated feed is one of the most convenient forms of oral administration of drugs in aquaculture because it allows to treat a large population of fish in an easy way. Ho...
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| Format: | Article |
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MDPI AG
2020-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/3/528 |
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| author | Elena Baralla Maria Vittoria Varoni Maria Nieddu Maria Piera Demontis Paolo Merella Caterina Burreddu Giovanni Garippa Gianpiero Boatto |
| author_facet | Elena Baralla Maria Vittoria Varoni Maria Nieddu Maria Piera Demontis Paolo Merella Caterina Burreddu Giovanni Garippa Gianpiero Boatto |
| author_sort | Elena Baralla |
| collection | DOAJ |
| description | Praziquantel (PZQ) is an anthelmintic drug used in humans and animals against Platyhelminthes and in aquaculture in the Far East. Medicated feed is one of the most convenient forms of oral administration of drugs in aquaculture because it allows to treat a large population of fish in an easy way. However, this treatment may lead to residues in fish intended for human consumption. In this study, a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed in order to verify the presence of PZQ in samples of <i>Sparus aurata</i> after oral administration of feed treated with PZQ. The method was validated according to international guidelines. It showed good recoveries, selectivity and sensitivity (LOD and LOQ were 3.0 and 9.3 ng/g, respectively), with precision and matrix effect values ≤ 15%. This method could also be applied to determine PZQ residue in other fish species and thus to evaluate the appropriate withdrawal time in treated fish intended for human consumption. |
| first_indexed | 2024-04-12T09:19:20Z |
| format | Article |
| id | doaj.art-7300922e746f436095afee1ae670bdbe |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-12T09:19:20Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-7300922e746f436095afee1ae670bdbe2022-12-22T03:38:42ZengMDPI AGAnimals2076-26152020-03-0110352810.3390/ani10030528ani10030528Determination of Praziquantel in <i>Sparus aurata</i> L. after Administration of Medicated Animal FeedElena Baralla0Maria Vittoria Varoni1Maria Nieddu2Maria Piera Demontis3Paolo Merella4Caterina Burreddu5Giovanni Garippa6Gianpiero Boatto7Department of Veterinary Medicine, University of Sassari, 07100 Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, 07100 Sassari, ItalyDepartment of Chemistry and Pharmacy, University of Sassari, 07100 Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, 07100 Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, 07100 Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, 07100 Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, 07100 Sassari, ItalyDepartment of Chemistry and Pharmacy, University of Sassari, 07100 Sassari, ItalyPraziquantel (PZQ) is an anthelmintic drug used in humans and animals against Platyhelminthes and in aquaculture in the Far East. Medicated feed is one of the most convenient forms of oral administration of drugs in aquaculture because it allows to treat a large population of fish in an easy way. However, this treatment may lead to residues in fish intended for human consumption. In this study, a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed in order to verify the presence of PZQ in samples of <i>Sparus aurata</i> after oral administration of feed treated with PZQ. The method was validated according to international guidelines. It showed good recoveries, selectivity and sensitivity (LOD and LOQ were 3.0 and 9.3 ng/g, respectively), with precision and matrix effect values ≤ 15%. This method could also be applied to determine PZQ residue in other fish species and thus to evaluate the appropriate withdrawal time in treated fish intended for human consumption.https://www.mdpi.com/2076-2615/10/3/528praziquantelmedicated animal feedfish muscleresidueslc-ms/ms |
| spellingShingle | Elena Baralla Maria Vittoria Varoni Maria Nieddu Maria Piera Demontis Paolo Merella Caterina Burreddu Giovanni Garippa Gianpiero Boatto Determination of Praziquantel in <i>Sparus aurata</i> L. after Administration of Medicated Animal Feed Animals praziquantel medicated animal feed fish muscle residues lc-ms/ms |
| title | Determination of Praziquantel in <i>Sparus aurata</i> L. after Administration of Medicated Animal Feed |
| title_full | Determination of Praziquantel in <i>Sparus aurata</i> L. after Administration of Medicated Animal Feed |
| title_fullStr | Determination of Praziquantel in <i>Sparus aurata</i> L. after Administration of Medicated Animal Feed |
| title_full_unstemmed | Determination of Praziquantel in <i>Sparus aurata</i> L. after Administration of Medicated Animal Feed |
| title_short | Determination of Praziquantel in <i>Sparus aurata</i> L. after Administration of Medicated Animal Feed |
| title_sort | determination of praziquantel in i sparus aurata i l after administration of medicated animal feed |
| topic | praziquantel medicated animal feed fish muscle residues lc-ms/ms |
| url | https://www.mdpi.com/2076-2615/10/3/528 |
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