Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic Inflammation
Background and ObjectiveChronic spontaneous urticaria (CSU) is a histamine-mediated inflammatory skin disease, and second-generation non-sedating H1-antihistamines (nsAH) at licensed doses have long been the first-line therapy in CSU. However, about 50% of patients are resistant to nsAH, and the pre...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.831489/full |
| _version_ | 1818157860340629504 |
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| author | Yao Song Yao Song Kena Dan Zhengqiu Yao Xi Yang Bangtao Chen Fei Hao |
| author_facet | Yao Song Yao Song Kena Dan Zhengqiu Yao Xi Yang Bangtao Chen Fei Hao |
| author_sort | Yao Song |
| collection | DOAJ |
| description | Background and ObjectiveChronic spontaneous urticaria (CSU) is a histamine-mediated inflammatory skin disease, and second-generation non-sedating H1-antihistamines (nsAH) at licensed doses have long been the first-line therapy in CSU. However, about 50% of patients are resistant to nsAH, and the precise pathogenesis remains largely unknown but seems to be associated with low-level systemic or intestinal inflammation. We aim to determine the fecal microbial composition and clarify its correlation with the clinical profiles og CSU with nsAH resistance.MethodsA total of 25 CSU patients with or 19 CSU patients without nsAH resistance and 19 healthy controls (HC) were enrolled in this study. The intestinal microbiome was detected by 16S rRNA sequencing. The data were analyzed using R language software.ResultsSignificantly higher urticarial activity score for 7 days, stool calprotectin, erythrocyte sedimentation rate, serum C-reactive protein, and interleukin-6, but much lower alpha-diversity and evenness of fecal bacterial community were observed in CSU patients with nsAH resistance than in those without (P <0.05 for all variables). Compared to patients with nsAH-responsiveness, the abundance of fecal genera Prevotella, Megamonas, and Escherichia were significantly increased, while that of Blautia, Alistipes, Anaerostipes, and Lachnospira were remarkably reduced in nsAH-resistant patients (uncorrected P <0.05 for all variables). Finally, systemic not intestinal inflammation degree was positively correlated with genera Escherichia, while negatively with genera Blautia, Dorea, Lactobacillus, Eubacterium_hallii_group, and Roseburia. CSU without nsAH resistance and HC individuals showed almost unchanged genera bacterium.ConclusionsAmong CSU patients, pro-inflammation phenotype relating to enteric dysbacteriosis features nsAH resistance in CSU patients. The results provide clues for future microbial-based or anti-inflammatory therapies on nsAH resistant CSU. |
| first_indexed | 2024-12-11T15:20:54Z |
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| id | doaj.art-73047d2e51e34c8ab1cef888cd15a303 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-11T15:20:54Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-73047d2e51e34c8ab1cef888cd15a3032022-12-22T01:00:22ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-03-011210.3389/fcimb.2022.831489831489Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic InflammationYao Song0Yao Song1Kena Dan2Zhengqiu Yao3Xi Yang4Bangtao Chen5Fei Hao6Department of Pediatrics, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Dermatology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Dermatology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Dermatology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Dermatology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Dermatology, Chongqing University Three Gorges Hospital, School of Medicine, Chong University, Chongqing, ChinaDepartment of Dermatology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaBackground and ObjectiveChronic spontaneous urticaria (CSU) is a histamine-mediated inflammatory skin disease, and second-generation non-sedating H1-antihistamines (nsAH) at licensed doses have long been the first-line therapy in CSU. However, about 50% of patients are resistant to nsAH, and the precise pathogenesis remains largely unknown but seems to be associated with low-level systemic or intestinal inflammation. We aim to determine the fecal microbial composition and clarify its correlation with the clinical profiles og CSU with nsAH resistance.MethodsA total of 25 CSU patients with or 19 CSU patients without nsAH resistance and 19 healthy controls (HC) were enrolled in this study. The intestinal microbiome was detected by 16S rRNA sequencing. The data were analyzed using R language software.ResultsSignificantly higher urticarial activity score for 7 days, stool calprotectin, erythrocyte sedimentation rate, serum C-reactive protein, and interleukin-6, but much lower alpha-diversity and evenness of fecal bacterial community were observed in CSU patients with nsAH resistance than in those without (P <0.05 for all variables). Compared to patients with nsAH-responsiveness, the abundance of fecal genera Prevotella, Megamonas, and Escherichia were significantly increased, while that of Blautia, Alistipes, Anaerostipes, and Lachnospira were remarkably reduced in nsAH-resistant patients (uncorrected P <0.05 for all variables). Finally, systemic not intestinal inflammation degree was positively correlated with genera Escherichia, while negatively with genera Blautia, Dorea, Lactobacillus, Eubacterium_hallii_group, and Roseburia. CSU without nsAH resistance and HC individuals showed almost unchanged genera bacterium.ConclusionsAmong CSU patients, pro-inflammation phenotype relating to enteric dysbacteriosis features nsAH resistance in CSU patients. The results provide clues for future microbial-based or anti-inflammatory therapies on nsAH resistant CSU.https://www.frontiersin.org/articles/10.3389/fcimb.2022.831489/fullgut microbiotachronic spontaneous urticaria (CSU)antihistamine resistanceinflammation16S rRNA sequencing |
| spellingShingle | Yao Song Yao Song Kena Dan Zhengqiu Yao Xi Yang Bangtao Chen Fei Hao Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic Inflammation Frontiers in Cellular and Infection Microbiology gut microbiota chronic spontaneous urticaria (CSU) antihistamine resistance inflammation 16S rRNA sequencing |
| title | Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic Inflammation |
| title_full | Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic Inflammation |
| title_fullStr | Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic Inflammation |
| title_full_unstemmed | Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic Inflammation |
| title_short | Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic Inflammation |
| title_sort | altered gut microbiota in h1 antihistamine resistant chronic spontaneous urticaria associates with systemic inflammation |
| topic | gut microbiota chronic spontaneous urticaria (CSU) antihistamine resistance inflammation 16S rRNA sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.831489/full |
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