Study of the Characterization of Partial Purified Polyamine Oxidase from Sheep's Brain Tissue
The study included partially purified of polyamine oxidase (PAO) from sheep's brain tissue by dialysis and ion exchange chromatography using DEAE-cellulose techniques. Tow isoenzymes (I،II) were obtained with specific activities 3.876 and 2.856 units/ mg of protein and purification folds 11, 8...
| Main Authors: | , |
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| Format: | Article |
| Language: | Arabic |
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College of Education for Pure Sciences
2019-09-01
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| Series: | مجلة التربية والعلم |
| Subjects: | |
| Online Access: | https://edusj.mosuljournals.com/article_162978_b6f4c27ecca3c379977ac7224c747702.pdf |
| _version_ | 1828749632241401856 |
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| author | Wathba Ali Sarah Younis |
| author_facet | Wathba Ali Sarah Younis |
| author_sort | Wathba Ali |
| collection | DOAJ |
| description | The study included partially purified of polyamine oxidase (PAO) from sheep's brain tissue by dialysis and ion exchange chromatography using DEAE-cellulose techniques. Tow isoenzymes (I،II) were obtained with specific activities 3.876 and 2.856 units/ mg of protein and purification folds 11, 8 times respectively compared with crude enzyme.<br />The specific activity of PAO I was better than PAO II, thus we considered it for following studying. The optimal condition showed 100 μL volume of enzyme, pH=9, 40ͦ C, at incubation time 10 min. Some of properties of partially purified PAO enzyme were studied and the specificity was found to be superior to the base material, where it was given the highest activity when using spermidine. Using lineweaver_ Burk plot, the values of maximum velocity (Vmax) and Michaelis constant (Km) were found to be 0.145 Unit/ ml and 43.4 mM respectively, and potassium ion was the most activator ion to the enzyme arrived to 233.8%. The compounds (ammonium chloride, sodium fluoride, phenyl hydrazine, sodium azide and EDTA) showed a significant inhibitory effect on the activity of the enzyme (56.1%, 49.9%, 49.9%, 37.3% and 25%) respectively. |
| first_indexed | 2024-12-10T20:25:46Z |
| format | Article |
| id | doaj.art-7307724e7f6d4232ab536a89813fff6c |
| institution | Directory Open Access Journal |
| issn | 1812-125X 2664-2530 |
| language | Arabic |
| last_indexed | 2024-12-10T20:25:46Z |
| publishDate | 2019-09-01 |
| publisher | College of Education for Pure Sciences |
| record_format | Article |
| series | مجلة التربية والعلم |
| spelling | doaj.art-7307724e7f6d4232ab536a89813fff6c2022-12-22T01:34:52ZaraCollege of Education for Pure Sciencesمجلة التربية والعلم1812-125X2664-25302019-09-0128361610.33899/edusj.2019.162978162978Study of the Characterization of Partial Purified Polyamine Oxidase from Sheep's Brain TissueWathba AliSarah YounisThe study included partially purified of polyamine oxidase (PAO) from sheep's brain tissue by dialysis and ion exchange chromatography using DEAE-cellulose techniques. Tow isoenzymes (I،II) were obtained with specific activities 3.876 and 2.856 units/ mg of protein and purification folds 11, 8 times respectively compared with crude enzyme.<br />The specific activity of PAO I was better than PAO II, thus we considered it for following studying. The optimal condition showed 100 μL volume of enzyme, pH=9, 40ͦ C, at incubation time 10 min. Some of properties of partially purified PAO enzyme were studied and the specificity was found to be superior to the base material, where it was given the highest activity when using spermidine. Using lineweaver_ Burk plot, the values of maximum velocity (Vmax) and Michaelis constant (Km) were found to be 0.145 Unit/ ml and 43.4 mM respectively, and potassium ion was the most activator ion to the enzyme arrived to 233.8%. The compounds (ammonium chloride, sodium fluoride, phenyl hydrazine, sodium azide and EDTA) showed a significant inhibitory effect on the activity of the enzyme (56.1%, 49.9%, 49.9%, 37.3% and 25%) respectively.https://edusj.mosuljournals.com/article_162978_b6f4c27ecca3c379977ac7224c747702.pdfpolyaminepolyamine oxidasesheep's brain tissue |
| spellingShingle | Wathba Ali Sarah Younis Study of the Characterization of Partial Purified Polyamine Oxidase from Sheep's Brain Tissue مجلة التربية والعلم polyamine polyamine oxidase sheep's brain tissue |
| title | Study of the Characterization of Partial Purified Polyamine Oxidase from Sheep's Brain Tissue |
| title_full | Study of the Characterization of Partial Purified Polyamine Oxidase from Sheep's Brain Tissue |
| title_fullStr | Study of the Characterization of Partial Purified Polyamine Oxidase from Sheep's Brain Tissue |
| title_full_unstemmed | Study of the Characterization of Partial Purified Polyamine Oxidase from Sheep's Brain Tissue |
| title_short | Study of the Characterization of Partial Purified Polyamine Oxidase from Sheep's Brain Tissue |
| title_sort | study of the characterization of partial purified polyamine oxidase from sheep s brain tissue |
| topic | polyamine polyamine oxidase sheep's brain tissue |
| url | https://edusj.mosuljournals.com/article_162978_b6f4c27ecca3c379977ac7224c747702.pdf |
| work_keys_str_mv | AT wathbaali studyofthecharacterizationofpartialpurifiedpolyamineoxidasefromsheepsbraintissue AT sarahyounis studyofthecharacterizationofpartialpurifiedpolyamineoxidasefromsheepsbraintissue |