Effect of N-acetyl-L-cysteine on inflammation after intraperitoneal mesh placement in a potentially contaminated environment: An experimental study in the rat
Background: The use of prosthetic meshes in abdominal wall reconstruction is a well-established approach; however, in certain cases where a bowel resection coexists its application is disputed. Any underlying inflammatory process may augment adhesion formation which is a major postoperative complica...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-11-01
|
| Series: | Asian Journal of Surgery |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1015958421006503 |
| _version_ | 1811180738846916608 |
|---|---|
| author | Styliani Parpoudi Ioannis Mantzoros Anna Gkiouliava Dimitrios Kyziridis Apostolos Makrantonakis Christos Chatzakis Christos Gekas Dimitrios Konstantaras Orestis Ioannidis Stefanos Bitsianis Dimosthenis Miliaras Stamatios Aggelopoulos |
| author_facet | Styliani Parpoudi Ioannis Mantzoros Anna Gkiouliava Dimitrios Kyziridis Apostolos Makrantonakis Christos Chatzakis Christos Gekas Dimitrios Konstantaras Orestis Ioannidis Stefanos Bitsianis Dimosthenis Miliaras Stamatios Aggelopoulos |
| author_sort | Styliani Parpoudi |
| collection | DOAJ |
| description | Background: The use of prosthetic meshes in abdominal wall reconstruction is a well-established approach; however, in certain cases where a bowel resection coexists its application is disputed. Any underlying inflammatory process may augment adhesion formation which is a major postoperative complication. In this animal study, our aim was to investigate the effect of N-acetyl-l-cysteine (NAC) on adhesion formation and the expression of inflammatory markers when a mesh was used in a clean or a potentially contaminated environment. Methods: Sixty male Wistar rats were randomly and equally allocated in 3 groups: A, B and C. Animals in all groups underwent laparotomy, a prosthetic mesh was placed and chemoprophylaxis with ciprofloxacin was administered. In groups B and C an enterectomy was also performed. NAC was injected intraperitoneally in group C. Adhesion formation, IL-1a, IL-6, TNF-a and histological data including fibrosis, neutrophils’ infiltration and neovascularization were assessed. Mesh samples were sent for cultivation. Results: Adhesion formation was significantly less and inflammation markers were also lower in group C compared to group B (p<0.05). Histological findings were significant for greater fibrosis, neutrophils’ infiltration and neovascularization in group B compared to both group A and C. Regarding mesh cultures, more specimens were tested positive in group B (p <0.05). Outcomes between group A and C did not differ. Conclusion: NAC effectively ameliorated adhesion formation and inflammation in a potentially septic environment where a prosthetic mesh was placed. |
| first_indexed | 2024-04-11T09:08:21Z |
| format | Article |
| id | doaj.art-7307ef0d39fb4d08885d6d180bfe13fe |
| institution | Directory Open Access Journal |
| issn | 1015-9584 |
| language | English |
| last_indexed | 2024-04-11T09:08:21Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Asian Journal of Surgery |
| spelling | doaj.art-7307ef0d39fb4d08885d6d180bfe13fe2022-12-22T04:32:34ZengElsevierAsian Journal of Surgery1015-95842022-11-01451121912196Effect of N-acetyl-L-cysteine on inflammation after intraperitoneal mesh placement in a potentially contaminated environment: An experimental study in the ratStyliani Parpoudi0Ioannis Mantzoros1Anna Gkiouliava2Dimitrios Kyziridis3Apostolos Makrantonakis4Christos Chatzakis5Christos Gekas6Dimitrios Konstantaras7Orestis Ioannidis8Stefanos Bitsianis9Dimosthenis Miliaras10Stamatios Aggelopoulos114th Surgical Clinic, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, Greece4th Surgical Clinic, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, GreeceAnaesthesiology Department, Georgios Papanikolaou General Hospital, Thessaloniki, Greece; Corresponding author.4th Surgical Clinic, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, Greece4th Surgical Clinic, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, Greece4th Surgical Clinic, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, GreeceOrthopaedic Department, Ippokrateio Hospital, Thessaloniki, Greece4th Surgical Clinic, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, Greece4th Surgical Clinic, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, Greece4th Surgical Clinic, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, GreeceLaboratory of Histology-Embryology, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, Greece4th Surgical Clinic, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Thessaloniki, GreeceBackground: The use of prosthetic meshes in abdominal wall reconstruction is a well-established approach; however, in certain cases where a bowel resection coexists its application is disputed. Any underlying inflammatory process may augment adhesion formation which is a major postoperative complication. In this animal study, our aim was to investigate the effect of N-acetyl-l-cysteine (NAC) on adhesion formation and the expression of inflammatory markers when a mesh was used in a clean or a potentially contaminated environment. Methods: Sixty male Wistar rats were randomly and equally allocated in 3 groups: A, B and C. Animals in all groups underwent laparotomy, a prosthetic mesh was placed and chemoprophylaxis with ciprofloxacin was administered. In groups B and C an enterectomy was also performed. NAC was injected intraperitoneally in group C. Adhesion formation, IL-1a, IL-6, TNF-a and histological data including fibrosis, neutrophils’ infiltration and neovascularization were assessed. Mesh samples were sent for cultivation. Results: Adhesion formation was significantly less and inflammation markers were also lower in group C compared to group B (p<0.05). Histological findings were significant for greater fibrosis, neutrophils’ infiltration and neovascularization in group B compared to both group A and C. Regarding mesh cultures, more specimens were tested positive in group B (p <0.05). Outcomes between group A and C did not differ. Conclusion: NAC effectively ameliorated adhesion formation and inflammation in a potentially septic environment where a prosthetic mesh was placed.http://www.sciencedirect.com/science/article/pii/S1015958421006503N-Acetyl-l-cysteineAdhesionsInflammationSurgical meshSurgicalSite infection |
| spellingShingle | Styliani Parpoudi Ioannis Mantzoros Anna Gkiouliava Dimitrios Kyziridis Apostolos Makrantonakis Christos Chatzakis Christos Gekas Dimitrios Konstantaras Orestis Ioannidis Stefanos Bitsianis Dimosthenis Miliaras Stamatios Aggelopoulos Effect of N-acetyl-L-cysteine on inflammation after intraperitoneal mesh placement in a potentially contaminated environment: An experimental study in the rat Asian Journal of Surgery N-Acetyl-l-cysteine Adhesions Inflammation Surgical mesh Surgical Site infection |
| title | Effect of N-acetyl-L-cysteine on inflammation after intraperitoneal mesh placement in a potentially contaminated environment: An experimental study in the rat |
| title_full | Effect of N-acetyl-L-cysteine on inflammation after intraperitoneal mesh placement in a potentially contaminated environment: An experimental study in the rat |
| title_fullStr | Effect of N-acetyl-L-cysteine on inflammation after intraperitoneal mesh placement in a potentially contaminated environment: An experimental study in the rat |
| title_full_unstemmed | Effect of N-acetyl-L-cysteine on inflammation after intraperitoneal mesh placement in a potentially contaminated environment: An experimental study in the rat |
| title_short | Effect of N-acetyl-L-cysteine on inflammation after intraperitoneal mesh placement in a potentially contaminated environment: An experimental study in the rat |
| title_sort | effect of n acetyl l cysteine on inflammation after intraperitoneal mesh placement in a potentially contaminated environment an experimental study in the rat |
| topic | N-Acetyl-l-cysteine Adhesions Inflammation Surgical mesh Surgical Site infection |
| url | http://www.sciencedirect.com/science/article/pii/S1015958421006503 |
| work_keys_str_mv | AT stylianiparpoudi effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT ioannismantzoros effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT annagkiouliava effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT dimitrioskyziridis effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT apostolosmakrantonakis effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT christoschatzakis effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT christosgekas effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT dimitrioskonstantaras effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT orestisioannidis effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT stefanosbitsianis effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT dimosthenismiliaras effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat AT stamatiosaggelopoulos effectofnacetyllcysteineoninflammationafterintraperitonealmeshplacementinapotentiallycontaminatedenvironmentanexperimentalstudyintherat |