Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis
Background: Ozone is a three-oxygen molecule (O3). Ozone therapy (OT) is systematically effective when pathological inflammatory and immunologic processes are activated. Among of these conditions are wound healing, macular degeneration related to aging, and conditions that are ischemic or infectious...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2014-09-01
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| Series: | Balkan Medical Journal |
| Subjects: | |
| Online Access: | http://balkanmedicaljournal.org/text.php?lang=en&id=294 |
| _version_ | 1797908889657671680 |
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| author | Başak Erginel Ali İhsan Dokucu Turgay Enginel Bilgin Aksoy |
| author_facet | Başak Erginel Ali İhsan Dokucu Turgay Enginel Bilgin Aksoy |
| author_sort | Başak Erginel |
| collection | DOAJ |
| description | Background: Ozone is a three-oxygen molecule (O3). Ozone therapy (OT) is systematically effective when pathological inflammatory and immunologic processes are activated. Among of these conditions are wound healing, macular degeneration related to aging, and conditions that are ischemic or infectious.
Aims: The aim of this study was to determine the effects of OT on
wound healing of intestinal anastomosis in the presence of peritonitis in a rat model.
Study Design: Animal experimentation.
Methods: A total of 40 Wistar albino rats were randomized into four groups (n=10) including: sham (S), peritonitis (P), ozone 0 (O0), and ozone 24 (O24). In group S, only cecal dissection was carried out. The S group had only a cecal dissection and intestinal anastomosis performed, but no peritonitis. In all other groups, cecal ligation and puncture (CLP) followed the cecal dissection to induce bacterial peritonitis. 24 h after puncture, a cecal resection and ileocolic anastomosis were performed. In group P, 24 h after CLP, a cecal resection and ileocolic anastomosis were performed and no ozone was administered.
In group O0, immediately after the anastomosis, and in group
O24, starting 24 hours after the anastomosis, an intraperitoneal 1 mg/kg/day ozone administration was applied for seven days. On the seventh day the animals were sacrificed, the anastomotic bursting pressures (BP) and the hydroxyproline values of the anastomotic tissues were measured, and histopathologic examination of the anastomotic segment was carried out.
Results: The highest BP was in group S, with 211±23.13 mmHg. The mean BP of group P was 141±56.25 mmHg, which was significantly lower than in the other two peritonitis groups that received ozone therapy, group O0 and O24, where it was 192±22 and 166±45 mmHg, respectively (p<0.05). The difference in the BP between groups O0 and O24 was not statistically significant (p>0.05). Histopathologic analyses of the anastomotic segments determined there was significantly more oedema and necrosis in the control group rats, and collagen deposition in the anastomotic tissue was significantly higher in the ozone-treated groups on postoperative day 7. Hydroxyproline levels were significantly higher in groups O0 and O24 compared to the peritonitis group (P).
Conclusion: Ozone therapy has a beneficial effect on anastomotic
healing of the colon in the presence of peritonitis.
(Balkan Med J 2014;31:249-53). |
| first_indexed | 2024-04-10T10:59:59Z |
| format | Article |
| id | doaj.art-73085ca82dca4690a961736fe6a34b08 |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T10:59:59Z |
| publishDate | 2014-09-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-73085ca82dca4690a961736fe6a34b082023-02-15T16:19:43ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312014-09-0131324925310.5152/balkanmedj.2014.13215Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of PeritonitisBaşak Erginel0Ali İhsan Dokucu1Turgay Enginel2Bilgin Aksoy3Department of Pediatric Surgery, İstanbul Şişli Etfal Training and Research Hospital, İstanbul, TurkeyDepartment of Pediatric Surgery, İstanbul Şişli Etfal Training and Research Hospital, İstanbul, TurkeyDepartment of General Surgery, İstanbul Training and Research Hospital, İstanbul, TurkeyDepartment of Pathology, İstanbul Training and Research Hospital, İstanbul, TurkeyBackground: Ozone is a three-oxygen molecule (O3). Ozone therapy (OT) is systematically effective when pathological inflammatory and immunologic processes are activated. Among of these conditions are wound healing, macular degeneration related to aging, and conditions that are ischemic or infectious. Aims: The aim of this study was to determine the effects of OT on wound healing of intestinal anastomosis in the presence of peritonitis in a rat model. Study Design: Animal experimentation. Methods: A total of 40 Wistar albino rats were randomized into four groups (n=10) including: sham (S), peritonitis (P), ozone 0 (O0), and ozone 24 (O24). In group S, only cecal dissection was carried out. The S group had only a cecal dissection and intestinal anastomosis performed, but no peritonitis. In all other groups, cecal ligation and puncture (CLP) followed the cecal dissection to induce bacterial peritonitis. 24 h after puncture, a cecal resection and ileocolic anastomosis were performed. In group P, 24 h after CLP, a cecal resection and ileocolic anastomosis were performed and no ozone was administered. In group O0, immediately after the anastomosis, and in group O24, starting 24 hours after the anastomosis, an intraperitoneal 1 mg/kg/day ozone administration was applied for seven days. On the seventh day the animals were sacrificed, the anastomotic bursting pressures (BP) and the hydroxyproline values of the anastomotic tissues were measured, and histopathologic examination of the anastomotic segment was carried out. Results: The highest BP was in group S, with 211±23.13 mmHg. The mean BP of group P was 141±56.25 mmHg, which was significantly lower than in the other two peritonitis groups that received ozone therapy, group O0 and O24, where it was 192±22 and 166±45 mmHg, respectively (p<0.05). The difference in the BP between groups O0 and O24 was not statistically significant (p>0.05). Histopathologic analyses of the anastomotic segments determined there was significantly more oedema and necrosis in the control group rats, and collagen deposition in the anastomotic tissue was significantly higher in the ozone-treated groups on postoperative day 7. Hydroxyproline levels were significantly higher in groups O0 and O24 compared to the peritonitis group (P). Conclusion: Ozone therapy has a beneficial effect on anastomotic healing of the colon in the presence of peritonitis. (Balkan Med J 2014;31:249-53).http://balkanmedicaljournal.org/text.php?lang=en&id=294Anastomosiscolonozoneperitonitisrat |
| spellingShingle | Başak Erginel Ali İhsan Dokucu Turgay Enginel Bilgin Aksoy Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis Balkan Medical Journal Anastomosis colon ozone peritonitis rat |
| title | Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis |
| title_full | Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis |
| title_fullStr | Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis |
| title_full_unstemmed | Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis |
| title_short | Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis |
| title_sort | effect of ozone therapy ot on healing of colonic anastomosis in a rat model of peritonitis |
| topic | Anastomosis colon ozone peritonitis rat |
| url | http://balkanmedicaljournal.org/text.php?lang=en&id=294 |
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