The collagen type I alpha 1 chain gene is an alternative safe harbor locus in the porcine genome

The collagen type I alpha 1 chain gene is an alternative safe harbor locus in the porcine genome

Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors. Safe harbor loci in the animal genome enable consistent overexpression of foreign genes, without side effects. However, relatively few safe...

Full description

Bibliographic Details
Main Authors:	Guang-ming XIANG, Xiu-ling ZHANG, Chang-jiang XU, Zi-yao FAN, Kui XU, Nan WANG, Yue WANG, Jing-jing CHE, Song-song XU, Yu-lian MU, Kui LI, Zhi-guo LIU
Format:	Article
Language:	English
Published:	Elsevier 2023-01-01
Series:	Journal of Integrative Agriculture
Subjects:	COL1A1 gene safe harbor knock-in CRISPR/Cas9 pig
Online Access:	http://www.sciencedirect.com/science/article/pii/S2095311922001836

Similar Items

Identification of the <i>CKM</i> Gene as a Potential Muscle-Specific Safe Harbor Locus in Pig Genome
by: Youcai Xiong, et al.
Published: (2022-05-01)

CRISPR/Cas9-Mediated Integration of Large Transgene into Pig CEP112 Locus
by: Guoling Li, et al.
Published: (2020-02-01)

Rapid Self-Selecting and Clone-Free Integration of Transgenes into Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans
by: Zachary C. Stevenson, et al.
Published: (2020-10-01)

CRISPR/Cas9-Mediated Specific Integration of <i>Fat-1</i> and <i>IGF-1</i> at the p<i>Rosa26</i> Locus
by: Wenni You, et al.
Published: (2021-07-01)

Integrating Omics and CRISPR Technology for Identification and Verification of Genomic Safe Harbor Loci in the Chicken Genome
by: Nima Dehdilani, et al.
Published: (2023-06-01)

A Multi-Faceted Approach for Prediction of Genome Safe Harbor Loci in the Chicken Genome
by: Nima Dehdilani, et al.
Published: (2021-09-01)

Quantification of transgene expression in GSH AAVS1 with a novel CRISPR/Cas9-based approach reveals high transcriptional variation
by: Anne Inderbitzin, et al.
Published: (2022-09-01)

Genome editing of pseudorabies virus in the CRISPR/Cas9 era: a mini-review
by: Hai-Ming Wang, et al.
Published: (2023-07-01)

PTENP1 is a ceRNA for PTEN: it’s CRISPR clear
by: Marianna Vitiello, et al.
Published: (2020-06-01)

Comparison of Multiple Strategies for Precision Transgene Knock-In in <i>Gallus gallus</i> Genome via Microhomology-Mediated End Joining
by: Lijuan Wang, et al.
Published: (2023-10-01)

Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering
by: Ikuko Watakabe, et al.
Published: (2018-02-01)

Identification of Genomic Safe Harbors in the Anhydrobiotic Cell Line, Pv11
by: Yugo Miyata, et al.
Published: (2022-02-01)

Efficient targeted integration directed by short homology in zebrafish and mammalian cells
by: Wesley A Wierson, et al.
Published: (2020-05-01)

Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications
by: Oliver Hertel, et al.
Published: (2022-10-01)

Report on small craft harbors /
by: 6189 American Society of Civil Engineers
Published: (1969)

Safe Harbor Limit; An Exposure Level Which Protects Consumers From Cancer Incidence
by: Masood A Shammas, et al.
Published: (2015-03-01)

CRISPR‐based knock‐in mutagenesis of the pioneer transcription factor FOXA1: optimization of strategies for multi‐allelic proteins in cancer cells
by: Shen Li, et al.
Published: (2021-06-01)

Establishment of an Integrated CRISPR/Cas9 Plasmid System for Simple and Efficient Genome Editing in Medaka In Vitro and In Vivo
by: Zeming Zhang, et al.
Published: (2023-02-01)

Genomics and epigenetics guided identification of tissue-specific genomic safe harbors
by: Dewan Shrestha, et al.
Published: (2022-09-01)

Successful production of genome-edited rats by the rGONAD method
by: Tomoe Kobayashi, et al.
Published: (2018-04-01)

Molecular Characteristics and Promoter Analysis of Porcine <i>COL1A1</i>
by: Guangming Xiang, et al.
Published: (2022-10-01)

Curing hemophilia A by NHEJ-mediated ectopic F8 insertion in the mouse
by: Jian-Ping Zhang, et al.
Published: (2019-12-01)

CRISPR/Cas Genome Editing—Can It Become a Game Changer in Future Fisheries Sector?
by: Suvra Roy, et al.
Published: (2022-07-01)

Establishment of PIN1 knockout porcine fetal fibroblast cell lines via CRISPR/Cas9 mediated gene-targeting
by: WANG Wanyi, et al.
Published: (2022-07-01)

Evaluating the feasibility of Cas9 overexpression in 3T3-L1 cells for generation of genetic knock-out adipocyte cell lines
by: Tomás Suchý, et al.
Published: (2021-01-01)

ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells
by: Akira Mabuchi, et al.
Published: (2023-05-01)

Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation
by: Carina Edmondson, et al.
Published: (2021-07-01)

Confirmation of a Causal Taar1 Allelic Variant in Addiction-Relevant Methamphetamine Behaviors
by: Tamara J. Phillips, et al.
Published: (2021-08-01)

Suppression of <i>SHROOM1</i> Improves In Vitro and In Vivo Gene Integration by Promoting Homology-Directed Repair
by: Zhihua Zhao, et al.
Published: (2020-08-01)

Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos
by: Maki Hirata, et al.
Published: (2021-02-01)

Highly efficient correction of structural mutations of 450 kb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9
by: Ke Qin, et al.
Published: (2019-04-01)

Porcine Knock-in Fibroblasts Expressing hDAF on α-1,3-Galactosyltransferase (GGTA1) Gene Locus
by: Ji Woo Kim, et al.
Published: (2012-10-01)

CRISPR Interference–Potential Application in Retinal Disease
by: Caroline F. Peddle, et al.
Published: (2020-03-01)

Target-AID-Mediated Multiplex Base Editing in Porcine Fibroblasts
by: Soo-Young Yum, et al.
Published: (2021-12-01)

CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance
by: Kui Xu, et al.
Published: (2020-09-01)

Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells
by: Natalia Kruglova, et al.
Published: (2024-01-01)

An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation
by: Yukiko U. Inoue, et al.
Published: (2021-04-01)

CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell Therapy
by: Zelda Odé, et al.
Published: (2020-06-01)

Calcium-Free and Cytochalasin B Treatment Inhibits Blastomere Fusion in 2-Cell Stage Embryos for the Generation of Floxed Mice via Sequential Electroporation
by: Takuro Horii, et al.
Published: (2020-04-01)

CRISPR/Cas9-Mediated Generation of COL7A1-Deficient Keratinocyte Model of Recessive Dystrophic Epidermolysis Bullosa
by: Farzad Alipour, et al.
Published: (2023-10-01)