The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi
The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used di...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga, Faculty of Veterinary Medicine
2022-10-01
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| Series: | Jurnal Medik Veteriner |
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| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/26796 |
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| author | Ryan David Pandapotan Hutahaean Dewa Ketut Meles Ratih Novita Praja Jola Rahmahani Prima Ayu Wibawati |
| author_facet | Ryan David Pandapotan Hutahaean Dewa Ketut Meles Ratih Novita Praja Jola Rahmahani Prima Ayu Wibawati |
| author_sort | Ryan David Pandapotan Hutahaean |
| collection | DOAJ |
| description | The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi. |
| first_indexed | 2024-04-12T03:22:00Z |
| format | Article |
| id | doaj.art-731528ccc9c14e098c900e211ca8029d |
| institution | Directory Open Access Journal |
| issn | 2615-7497 2581-012X |
| language | English |
| last_indexed | 2024-04-12T03:22:00Z |
| publishDate | 2022-10-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj.art-731528ccc9c14e098c900e211ca8029d2022-12-22T03:49:51ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2022-10-015222523310.20473/jmv.vol5.iss2.2022.225-23321914The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, BanyuwangiRyan David Pandapotan Hutahaean0Dewa Ketut Meles1https://orcid.org/0000-0003-4071-9098Ratih Novita Praja2https://orcid.org/0000-0002-0239-2939Jola Rahmahani3https://orcid.org/0000-0001-9103-3200Prima Ayu Wibawati4https://orcid.org/0000-0002-5950-118XMahasiswa Pendidikan Dokter HewanDepartemen Kedokteran DasarDepartemen Mikrobiologi VeterinerDepartemen Mikrobiologi VeterinerDepartemen Kesehatan Masyarakat VeterinerThe aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.https://e-journal.unair.ac.id/JMV/article/view/26796antibacterialescherichia coligliricidia sepiumlepidochelys olivacea |
| spellingShingle | Ryan David Pandapotan Hutahaean Dewa Ketut Meles Ratih Novita Praja Jola Rahmahani Prima Ayu Wibawati The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi Jurnal Medik Veteriner antibacterial escherichia coli gliricidia sepium lepidochelys olivacea |
| title | The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi |
| title_full | The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi |
| title_fullStr | The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi |
| title_full_unstemmed | The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi |
| title_short | The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi |
| title_sort | effect of the gamal leaf ethanol extract on the growth of escherichia coli isolated from semi natural sea turtle nest in boom beach banyuwangi |
| topic | antibacterial escherichia coli gliricidia sepium lepidochelys olivacea |
| url | https://e-journal.unair.ac.id/JMV/article/view/26796 |
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