QUANTITATIVE RHOA Gly17Val ALLELE-SPECIFIC POLYMERASE CHAIN REACTION AND T-CELL CLONALITY ANALYSIS IN ANGIOIMMUNOBLASTIC T-CELL LYMPHOMA
Introduction. Аngioimmunoblastic T-cell lymphoma (AITL) is a T-cell lymphoma, characterized by abundant polymorphocellular infiltrate of lymph nodes with the small number of tumor CD4+ T-cells. AITL could often be misdiagnosed as reactive processes and other lymphomas, including Hodgkin’s lymphoma....
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| Format: | Article |
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ABV-press
2018-02-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/266 |
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| author | Yu. V. Sidorova N. G. Chernova I. A. Yakutik S. Yu. Smirnova N. V. Ryzhikova E. E. Nikulina M. Yu. Aleksenko M. N. Sinitsyna A. M. Kovrigina T. N. Moiseeva E. E. Zvonkov A. B. Sudarikov |
| author_facet | Yu. V. Sidorova N. G. Chernova I. A. Yakutik S. Yu. Smirnova N. V. Ryzhikova E. E. Nikulina M. Yu. Aleksenko M. N. Sinitsyna A. M. Kovrigina T. N. Moiseeva E. E. Zvonkov A. B. Sudarikov |
| author_sort | Yu. V. Sidorova |
| collection | DOAJ |
| description | Introduction. Аngioimmunoblastic T-cell lymphoma (AITL) is a T-cell lymphoma, characterized by abundant polymorphocellular infiltrate of lymph nodes with the small number of tumor CD4+ T-cells. AITL could often be misdiagnosed as reactive processes and other lymphomas, including Hodgkin’s lymphoma. Molecular methods of determining clonality are used to confirm the diagnosis. TCR gene rearrangements detected in the lymph nodes may not coincide with those detected in the bone marrow (BM), blood or skin, that makes their interpretation difficult. Recently discovered point somatic RHOA (Ras homolog gene family, member A) Gly17Val mutation is present in 53–71 % of angioimmunoblastic T-cell lymphomas.The aim of the study was to evaluate the number of RHOA Gly17Val mutated cells in different tissues of AITL patients and to correlate it with corresponding T-cell clonality results.Materials and methods. TCRG and TCRB gene rearrangements were PCR-amplified according to BIOMED-2 standardized protocol and analyzed by capillary electrophoresis on ABI Prism 3130. Gly17Val mutation was analyzed by quantitative allele-specific (qAS) TaqMan Real-Time PCR assay. Lymph nodes (LN) and skin biopsies, blood and BM samples were studied for 40 patients with AITL.Results. The clonal TCR gene rearrangements were found in 37 (92 %) of 40 patients in LN and in 26 (96 %) of 28 patients in BM, but they were not matched in length in LN and BM in 12 (46 %) of 26 patients. RHOA (Gly17Val) mutation in LN was revealed in 60 % (24 of 40) patients. Number of cells with RHOA mutation was highest in the LN (in average 26.7 % of the total cells), while in the BM RHOA mutation were undetectable (in 7 patients), or detected in 10 patients in a low quantity (in average 2 % of the total cells). Skin, blood and BM samples with the T-cell clonality peaks that differ from those found in the LN were also negative for the presence of cells having RHOA Gly17Val mutation. Also, in four patients we showed that clonal products in BM and blood that do not coincide with LN refer to CD8+ lymphocytes.Conclusions. The results of the study demonstrate that the quantitative determination of cells with the mutation of RHOA Gly17Val must be taken into account in staging the disease and interpreting the results of T-cell clonality assay. Clonal cells with rearrangements not matching those identified for the LN should be considered reactive. |
| first_indexed | 2024-04-09T20:25:29Z |
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| language | Russian |
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| spelling | doaj.art-73162c18c8d84ab69fdae30025197d1d2023-03-30T20:15:11ZrusABV-pressОнкогематология1818-83462413-40232018-02-01124414910.17650/1818-8346-2017-12-4-41-49253QUANTITATIVE RHOA Gly17Val ALLELE-SPECIFIC POLYMERASE CHAIN REACTION AND T-CELL CLONALITY ANALYSIS IN ANGIOIMMUNOBLASTIC T-CELL LYMPHOMAYu. V. Sidorova0N. G. Chernova1I. A. Yakutik2S. Yu. Smirnova3N. V. Ryzhikova4E. E. Nikulina5M. Yu. Aleksenko6M. N. Sinitsyna7A. M. Kovrigina8T. N. Moiseeva9E. E. Zvonkov10A. B. Sudarikov11National Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaNational Research Center for Hematology, Ministry of Health of RussiaIntroduction. Аngioimmunoblastic T-cell lymphoma (AITL) is a T-cell lymphoma, characterized by abundant polymorphocellular infiltrate of lymph nodes with the small number of tumor CD4+ T-cells. AITL could often be misdiagnosed as reactive processes and other lymphomas, including Hodgkin’s lymphoma. Molecular methods of determining clonality are used to confirm the diagnosis. TCR gene rearrangements detected in the lymph nodes may not coincide with those detected in the bone marrow (BM), blood or skin, that makes their interpretation difficult. Recently discovered point somatic RHOA (Ras homolog gene family, member A) Gly17Val mutation is present in 53–71 % of angioimmunoblastic T-cell lymphomas.The aim of the study was to evaluate the number of RHOA Gly17Val mutated cells in different tissues of AITL patients and to correlate it with corresponding T-cell clonality results.Materials and methods. TCRG and TCRB gene rearrangements were PCR-amplified according to BIOMED-2 standardized protocol and analyzed by capillary electrophoresis on ABI Prism 3130. Gly17Val mutation was analyzed by quantitative allele-specific (qAS) TaqMan Real-Time PCR assay. Lymph nodes (LN) and skin biopsies, blood and BM samples were studied for 40 patients with AITL.Results. The clonal TCR gene rearrangements were found in 37 (92 %) of 40 patients in LN and in 26 (96 %) of 28 patients in BM, but they were not matched in length in LN and BM in 12 (46 %) of 26 patients. RHOA (Gly17Val) mutation in LN was revealed in 60 % (24 of 40) patients. Number of cells with RHOA mutation was highest in the LN (in average 26.7 % of the total cells), while in the BM RHOA mutation were undetectable (in 7 patients), or detected in 10 patients in a low quantity (in average 2 % of the total cells). Skin, blood and BM samples with the T-cell clonality peaks that differ from those found in the LN were also negative for the presence of cells having RHOA Gly17Val mutation. Also, in four patients we showed that clonal products in BM and blood that do not coincide with LN refer to CD8+ lymphocytes.Conclusions. The results of the study demonstrate that the quantitative determination of cells with the mutation of RHOA Gly17Val must be taken into account in staging the disease and interpreting the results of T-cell clonality assay. Clonal cells with rearrangements not matching those identified for the LN should be considered reactive.https://oncohematology.abvpress.ru/ongm/article/view/266аngioimmunoblastic t-cell lymphomaclonalitypoint mutationallele-specific polymerase chain reactionrhoa gly17val |
| spellingShingle | Yu. V. Sidorova N. G. Chernova I. A. Yakutik S. Yu. Smirnova N. V. Ryzhikova E. E. Nikulina M. Yu. Aleksenko M. N. Sinitsyna A. M. Kovrigina T. N. Moiseeva E. E. Zvonkov A. B. Sudarikov QUANTITATIVE RHOA Gly17Val ALLELE-SPECIFIC POLYMERASE CHAIN REACTION AND T-CELL CLONALITY ANALYSIS IN ANGIOIMMUNOBLASTIC T-CELL LYMPHOMA Онкогематология аngioimmunoblastic t-cell lymphoma clonality point mutation allele-specific polymerase chain reaction rhoa gly17val |
| title | QUANTITATIVE RHOA Gly17Val ALLELE-SPECIFIC POLYMERASE CHAIN REACTION AND T-CELL CLONALITY ANALYSIS IN ANGIOIMMUNOBLASTIC T-CELL LYMPHOMA |
| title_full | QUANTITATIVE RHOA Gly17Val ALLELE-SPECIFIC POLYMERASE CHAIN REACTION AND T-CELL CLONALITY ANALYSIS IN ANGIOIMMUNOBLASTIC T-CELL LYMPHOMA |
| title_fullStr | QUANTITATIVE RHOA Gly17Val ALLELE-SPECIFIC POLYMERASE CHAIN REACTION AND T-CELL CLONALITY ANALYSIS IN ANGIOIMMUNOBLASTIC T-CELL LYMPHOMA |
| title_full_unstemmed | QUANTITATIVE RHOA Gly17Val ALLELE-SPECIFIC POLYMERASE CHAIN REACTION AND T-CELL CLONALITY ANALYSIS IN ANGIOIMMUNOBLASTIC T-CELL LYMPHOMA |
| title_short | QUANTITATIVE RHOA Gly17Val ALLELE-SPECIFIC POLYMERASE CHAIN REACTION AND T-CELL CLONALITY ANALYSIS IN ANGIOIMMUNOBLASTIC T-CELL LYMPHOMA |
| title_sort | quantitative rhoa gly17val allele specific polymerase chain reaction and t cell clonality analysis in angioimmunoblastic t cell lymphoma |
| topic | аngioimmunoblastic t-cell lymphoma clonality point mutation allele-specific polymerase chain reaction rhoa gly17val |
| url | https://oncohematology.abvpress.ru/ongm/article/view/266 |
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