Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkers

The widespread use of titanium dioxide nanoparticles (TiO2 NPs) in many applications has led to its significant release to aquatic systems. Hence, the present study was performed to evaluate effects of TiO2 NPs on crayfish Procambarus clarkii, which is often used as a bioindicator of water pollution...

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Main Authors: Mahmoud Abd El-Atti, Mahmoud M.A. Desouky, Amaal Mohamadien, Radwa M. Said
Format: Article
Language:English
Published: Elsevier 2019-03-01
Series:Egyptian Journal of Aquatic Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1687428519300019
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author Mahmoud Abd El-Atti
Mahmoud M.A. Desouky
Amaal Mohamadien
Radwa M. Said
author_facet Mahmoud Abd El-Atti
Mahmoud M.A. Desouky
Amaal Mohamadien
Radwa M. Said
author_sort Mahmoud Abd El-Atti
collection DOAJ
description The widespread use of titanium dioxide nanoparticles (TiO2 NPs) in many applications has led to its significant release to aquatic systems. Hence, the present study was performed to evaluate effects of TiO2 NPs on crayfish Procambarus clarkii, which is often used as a bioindicator of water pollution. Adult male specimens were treated with 25, 125, and 250 mg/l of TiO2 NPs for 28 days. Mortalities were 0, 3.3, and 10% for animals treated with 25, 125, and 250 mg/l of TiO2 NPs, respectively. The level of titanium bioaccumulation in different tissues was as follows, gills > hepatopancreas > green glands > muscles. TiO2 NPs-exposed crayfish showed significant increase in the levels of catalase, glutathione-S-transferase, glutathione peroxidase, gamma-glutamyl transferase, glutathione, and metallothioneins in the hepatopancreas. Exposure of the crayfish to 250 mg/l TiO2 NPs caused severe histopathological alterations. In the hepatopancreas, the most prominent pathological changes were tubular disruption and inflammatory infiltration. Under transmission electron microscope (TEM), ruptured microvilli, deformed mitochondria and fragmentation of rough endoplasmic reticulum (RER) were observed. In the gills, swelling of gill lamellae, disorganization and degeneration of epithelial cells were noted under the light microscope (LM). Under electron microscope (EM), gills of crayfish treated with 250 mg/l TiO2 NPs showed vacuolation, dense granules, and diminution in the number of apical plasma membrane infoldings. In conclusion, TiO2 NPs caused alteration in antioxidant activities and severe histopathological changes. Keywords: Red swamp crayfish, Procambarus clarkii, Titanium dioxide nanoparticles, Antioxidant parameters, Histopathology, Hepatopancreas, Gills
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spelling doaj.art-7317725fe0b947e28c9f0e4e616c4da72022-12-21T20:02:53ZengElsevierEgyptian Journal of Aquatic Research1687-42852019-03-014511118Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkersMahmoud Abd El-Atti0Mahmoud M.A. Desouky1Amaal Mohamadien2Radwa M. Said3Department of Zoology, Faculty of Science, Zagazig University, Zagazig, EgyptDepartment of Zoology, Faculty of Science, Zagazig University, Zagazig, Egypt; Corresponding author.Department of Zoology, Faculty of Science, Zagazig University, Zagazig, Egypt; Department of Biology, Faculty of Science, Tai’f University, Saudi ArabiaDepartment of Zoology, Faculty of Science, Zagazig University, Zagazig, EgyptThe widespread use of titanium dioxide nanoparticles (TiO2 NPs) in many applications has led to its significant release to aquatic systems. Hence, the present study was performed to evaluate effects of TiO2 NPs on crayfish Procambarus clarkii, which is often used as a bioindicator of water pollution. Adult male specimens were treated with 25, 125, and 250 mg/l of TiO2 NPs for 28 days. Mortalities were 0, 3.3, and 10% for animals treated with 25, 125, and 250 mg/l of TiO2 NPs, respectively. The level of titanium bioaccumulation in different tissues was as follows, gills > hepatopancreas > green glands > muscles. TiO2 NPs-exposed crayfish showed significant increase in the levels of catalase, glutathione-S-transferase, glutathione peroxidase, gamma-glutamyl transferase, glutathione, and metallothioneins in the hepatopancreas. Exposure of the crayfish to 250 mg/l TiO2 NPs caused severe histopathological alterations. In the hepatopancreas, the most prominent pathological changes were tubular disruption and inflammatory infiltration. Under transmission electron microscope (TEM), ruptured microvilli, deformed mitochondria and fragmentation of rough endoplasmic reticulum (RER) were observed. In the gills, swelling of gill lamellae, disorganization and degeneration of epithelial cells were noted under the light microscope (LM). Under electron microscope (EM), gills of crayfish treated with 250 mg/l TiO2 NPs showed vacuolation, dense granules, and diminution in the number of apical plasma membrane infoldings. In conclusion, TiO2 NPs caused alteration in antioxidant activities and severe histopathological changes. Keywords: Red swamp crayfish, Procambarus clarkii, Titanium dioxide nanoparticles, Antioxidant parameters, Histopathology, Hepatopancreas, Gillshttp://www.sciencedirect.com/science/article/pii/S1687428519300019
spellingShingle Mahmoud Abd El-Atti
Mahmoud M.A. Desouky
Amaal Mohamadien
Radwa M. Said
Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkers
Egyptian Journal of Aquatic Research
title Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkers
title_full Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkers
title_fullStr Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkers
title_full_unstemmed Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkers
title_short Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkers
title_sort effects of titanium dioxide nanoparticles on red swamp crayfish procambarus clarkii bioaccumulation oxidative stress and histopathological biomarkers
url http://www.sciencedirect.com/science/article/pii/S1687428519300019
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