Oxidative Stress and Antioxidant Responses of <i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i> Under Diurnally Varying Light Conditions
Two harmful cyanobacteria species (<i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i>) were exposed to diurnal light-intensity variation to investigate their favorable and stressed phases during a single day. The photosynthetically active radiation (PAR) started at...
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MDPI AG
2020-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/6/890 |
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| author | Guligena Muhetaer Senavirathna M.D.H. Jayasanka Takeshi Fujino |
| author_facet | Guligena Muhetaer Senavirathna M.D.H. Jayasanka Takeshi Fujino |
| author_sort | Guligena Muhetaer |
| collection | DOAJ |
| description | Two harmful cyanobacteria species (<i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i>) were exposed to diurnal light-intensity variation to investigate their favorable and stressed phases during a single day. The photosynthetically active radiation (PAR) started at 0 µmol·m<sup>−2</sup>·s<sup>−1</sup> (06:00 h), increased by ~25 µmol·m<sup>−2</sup>·s<sup>−1 </sup>or ~50 µmol·m<sup>−2</sup>·s<sup>−1</sup> every 30 min, peaking at 300 µmol·m<sup>−2</sup>·s<sup>−1 </sup>or 600 µmol·m<sup>−2</sup>·s<sup>−1</sup> (12:00 h), and then decreased to 0 µmol·m<sup>−2</sup>·s<sup>−1</sup> (by 18:00 h). The H<sub>2</sub>O<sub>2</sub> and antioxidant activities were paralleled to light intensity. Higher H<sub>2</sub>O<sub>2</sub> and antioxidant levels (guaiacol peroxidase, catalase (CAT), and superoxidase dismutase) were observed at 600 µmol·m<sup>−2</sup>·s<sup>−1</sup> rather than at 300 µmol·m<sup>−2</sup>·s<sup>−1</sup>. Changes in antioxidant levels under each light condition differed between the species. Significant correlations were observed between antioxidant activities and H<sub>2</sub>O<sub>2</sub> contents for both species, except for the CAT activity of <i>P. ambiguum</i> at 300 µmol·m<sup>−2</sup>·s<sup>−1</sup>. Under each of the conditions, both species responded proportionately to oxidative stress. Even under maximum light intensities (300 µmol·m<sup>−2</sup>·s<sup>−1 </sup>or 600 µmol·m<sup>−2</sup>·s<sup>−1</sup> PAR intensity), neither species was stressed. Studies using extended exposure durations are warranted to better understand the growth performance and long-term physiological responses of both species. |
| first_indexed | 2024-03-10T19:13:02Z |
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| spelling | doaj.art-73190a9351f94fe2b7bba455c528c1902023-11-20T03:37:58ZengMDPI AGMicroorganisms2076-26072020-06-018689010.3390/microorganisms8060890Oxidative Stress and Antioxidant Responses of <i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i> Under Diurnally Varying Light ConditionsGuligena Muhetaer0Senavirathna M.D.H. Jayasanka1Takeshi Fujino2Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, JapanGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, JapanGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, JapanTwo harmful cyanobacteria species (<i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i>) were exposed to diurnal light-intensity variation to investigate their favorable and stressed phases during a single day. The photosynthetically active radiation (PAR) started at 0 µmol·m<sup>−2</sup>·s<sup>−1</sup> (06:00 h), increased by ~25 µmol·m<sup>−2</sup>·s<sup>−1 </sup>or ~50 µmol·m<sup>−2</sup>·s<sup>−1</sup> every 30 min, peaking at 300 µmol·m<sup>−2</sup>·s<sup>−1 </sup>or 600 µmol·m<sup>−2</sup>·s<sup>−1</sup> (12:00 h), and then decreased to 0 µmol·m<sup>−2</sup>·s<sup>−1</sup> (by 18:00 h). The H<sub>2</sub>O<sub>2</sub> and antioxidant activities were paralleled to light intensity. Higher H<sub>2</sub>O<sub>2</sub> and antioxidant levels (guaiacol peroxidase, catalase (CAT), and superoxidase dismutase) were observed at 600 µmol·m<sup>−2</sup>·s<sup>−1</sup> rather than at 300 µmol·m<sup>−2</sup>·s<sup>−1</sup>. Changes in antioxidant levels under each light condition differed between the species. Significant correlations were observed between antioxidant activities and H<sub>2</sub>O<sub>2</sub> contents for both species, except for the CAT activity of <i>P. ambiguum</i> at 300 µmol·m<sup>−2</sup>·s<sup>−1</sup>. Under each of the conditions, both species responded proportionately to oxidative stress. Even under maximum light intensities (300 µmol·m<sup>−2</sup>·s<sup>−1 </sup>or 600 µmol·m<sup>−2</sup>·s<sup>−1</sup> PAR intensity), neither species was stressed. Studies using extended exposure durations are warranted to better understand the growth performance and long-term physiological responses of both species.https://www.mdpi.com/2076-2607/8/6/890harmful cyanobacteriahydrogen peroxidespecies characteristicslinear relationshipsphoto stress |
| spellingShingle | Guligena Muhetaer Senavirathna M.D.H. Jayasanka Takeshi Fujino Oxidative Stress and Antioxidant Responses of <i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i> Under Diurnally Varying Light Conditions Microorganisms harmful cyanobacteria hydrogen peroxide species characteristics linear relationships photo stress |
| title | Oxidative Stress and Antioxidant Responses of <i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i> Under Diurnally Varying Light Conditions |
| title_full | Oxidative Stress and Antioxidant Responses of <i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i> Under Diurnally Varying Light Conditions |
| title_fullStr | Oxidative Stress and Antioxidant Responses of <i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i> Under Diurnally Varying Light Conditions |
| title_full_unstemmed | Oxidative Stress and Antioxidant Responses of <i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i> Under Diurnally Varying Light Conditions |
| title_short | Oxidative Stress and Antioxidant Responses of <i>Phormidium ambiguum</i> and <i>Microcystis aeruginosa</i> Under Diurnally Varying Light Conditions |
| title_sort | oxidative stress and antioxidant responses of i phormidium ambiguum i and i microcystis aeruginosa i under diurnally varying light conditions |
| topic | harmful cyanobacteria hydrogen peroxide species characteristics linear relationships photo stress |
| url | https://www.mdpi.com/2076-2607/8/6/890 |
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