Complement Activation in Patients With Diabetic Nephropathy
Complement activation plays a role in various organs in patients with diabetes. However, in diabetic nephropathy (DN), the role of complement activation is poorly understood. We examined the prevalence and clinical significance of complement deposits in the renal tissue of cases with type 1 and type...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-03-01
|
| Series: | Kidney International Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468024917304230 |
| _version_ | 1819161173850849280 |
|---|---|
| author | Pascal Bus Jamie S. Chua Céline Q.F. Klessens Malu Zandbergen Ron Wolterbeek Cees van Kooten Leendert A. Trouw Jan A. Bruijn Hans J. Baelde |
| author_facet | Pascal Bus Jamie S. Chua Céline Q.F. Klessens Malu Zandbergen Ron Wolterbeek Cees van Kooten Leendert A. Trouw Jan A. Bruijn Hans J. Baelde |
| author_sort | Pascal Bus |
| collection | DOAJ |
| description | Complement activation plays a role in various organs in patients with diabetes. However, in diabetic nephropathy (DN), the role of complement activation is poorly understood. We examined the prevalence and clinical significance of complement deposits in the renal tissue of cases with type 1 and type 2 diabetes with and without DN.
Methods: We measured the prevalence of glomerular C4d, C1q, mannose-binding lectin (MBL), and C5b-9 deposits in 101 autopsied diabetic cases with DN, 59 autopsied diabetic cases without DN, and 41 autopsied cases without diabetes or kidney disease. The presence of complement deposits was scored by researchers who were blinded with respect to the clinical and histological data.
Results: C4d deposits were more prevalent in cases with DN than in cases without DN in both the glomeruli (46% vs. 26%) and the arterioles (28% vs. 12%). C1q deposits were also increased in the glomerular hili (77% vs. 55%) and arterioles (33% vs.14%), and were correlated with DN (P < 0.01). MBL deposits were only rarely observed. C5b-9 deposits were more prevalent in the cases with diabetes mellitus (DM) than in the cases without DM (69% vs. 32%; P < 0.001). Finally, glomerular C4d and C5b-9 deposits were correlated with the severity of DN (ρ = 0.341 and 0.259, respectively; P < 0.001).
Conclusion: Complement activation is correlated with both the presence and severity of DN, suggesting that the complement system is involved in the development of renal pathology in patients with diabetes and is a promising target for inhibiting and/or preventing DN in these patients. |
| first_indexed | 2024-12-22T17:08:08Z |
| format | Article |
| id | doaj.art-731a58d0746246f8bf1f96f346fdf8bd |
| institution | Directory Open Access Journal |
| issn | 2468-0249 |
| language | English |
| last_indexed | 2024-12-22T17:08:08Z |
| publishDate | 2018-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Kidney International Reports |
| spelling | doaj.art-731a58d0746246f8bf1f96f346fdf8bd2022-12-21T18:19:08ZengElsevierKidney International Reports2468-02492018-03-013230231310.1016/j.ekir.2017.10.005Complement Activation in Patients With Diabetic NephropathyPascal Bus0Jamie S. Chua1Céline Q.F. Klessens2Malu Zandbergen3Ron Wolterbeek4Cees van Kooten5Leendert A. Trouw6Jan A. Bruijn7Hans J. Baelde8Department of Pathology, Leiden University Medical Center, Leiden, The NetherlandsDepartment of Pathology, Leiden University Medical Center, Leiden, The NetherlandsDepartment of Pathology, Leiden University Medical Center, Leiden, The NetherlandsDepartment of Pathology, Leiden University Medical Center, Leiden, The NetherlandsDepartment of Medical Statistics and Bioinformatics, Leiden University Medical Center, Leiden, The NetherlandsDepartment of Nephrology, Leiden University Medical Center, Leiden, The NetherlandsDepartment of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The NetherlandsDepartment of Pathology, Leiden University Medical Center, Leiden, The NetherlandsDepartment of Pathology, Leiden University Medical Center, Leiden, The NetherlandsComplement activation plays a role in various organs in patients with diabetes. However, in diabetic nephropathy (DN), the role of complement activation is poorly understood. We examined the prevalence and clinical significance of complement deposits in the renal tissue of cases with type 1 and type 2 diabetes with and without DN. Methods: We measured the prevalence of glomerular C4d, C1q, mannose-binding lectin (MBL), and C5b-9 deposits in 101 autopsied diabetic cases with DN, 59 autopsied diabetic cases without DN, and 41 autopsied cases without diabetes or kidney disease. The presence of complement deposits was scored by researchers who were blinded with respect to the clinical and histological data. Results: C4d deposits were more prevalent in cases with DN than in cases without DN in both the glomeruli (46% vs. 26%) and the arterioles (28% vs. 12%). C1q deposits were also increased in the glomerular hili (77% vs. 55%) and arterioles (33% vs.14%), and were correlated with DN (P < 0.01). MBL deposits were only rarely observed. C5b-9 deposits were more prevalent in the cases with diabetes mellitus (DM) than in the cases without DM (69% vs. 32%; P < 0.001). Finally, glomerular C4d and C5b-9 deposits were correlated with the severity of DN (ρ = 0.341 and 0.259, respectively; P < 0.001). Conclusion: Complement activation is correlated with both the presence and severity of DN, suggesting that the complement system is involved in the development of renal pathology in patients with diabetes and is a promising target for inhibiting and/or preventing DN in these patients.http://www.sciencedirect.com/science/article/pii/S2468024917304230C4dcomplement activationdiabetic nephropathyhistological lesionsrenal pathology |
| spellingShingle | Pascal Bus Jamie S. Chua Céline Q.F. Klessens Malu Zandbergen Ron Wolterbeek Cees van Kooten Leendert A. Trouw Jan A. Bruijn Hans J. Baelde Complement Activation in Patients With Diabetic Nephropathy Kidney International Reports C4d complement activation diabetic nephropathy histological lesions renal pathology |
| title | Complement Activation in Patients With Diabetic Nephropathy |
| title_full | Complement Activation in Patients With Diabetic Nephropathy |
| title_fullStr | Complement Activation in Patients With Diabetic Nephropathy |
| title_full_unstemmed | Complement Activation in Patients With Diabetic Nephropathy |
| title_short | Complement Activation in Patients With Diabetic Nephropathy |
| title_sort | complement activation in patients with diabetic nephropathy |
| topic | C4d complement activation diabetic nephropathy histological lesions renal pathology |
| url | http://www.sciencedirect.com/science/article/pii/S2468024917304230 |
| work_keys_str_mv | AT pascalbus complementactivationinpatientswithdiabeticnephropathy AT jamieschua complementactivationinpatientswithdiabeticnephropathy AT celineqfklessens complementactivationinpatientswithdiabeticnephropathy AT maluzandbergen complementactivationinpatientswithdiabeticnephropathy AT ronwolterbeek complementactivationinpatientswithdiabeticnephropathy AT ceesvankooten complementactivationinpatientswithdiabeticnephropathy AT leendertatrouw complementactivationinpatientswithdiabeticnephropathy AT janabruijn complementactivationinpatientswithdiabeticnephropathy AT hansjbaelde complementactivationinpatientswithdiabeticnephropathy |