Authentication of the R06E Fruit Bat Cell Line
Fruit bats and insectivorous bats are believed to provide a natural reservoir for a wide variety of infectious diseases. Several lines of evidence, including the successful isolation of infectious viruses, indicate that Marburg virus and Ravn virus have found a major reservoir in colonies of the Egy...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-05-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1999-4915/4/5/889 |
| _version_ | 1818297098952507392 |
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| author | Ingo Jordan Volker Sandig Vincent J. Munster |
| author_facet | Ingo Jordan Volker Sandig Vincent J. Munster |
| author_sort | Ingo Jordan |
| collection | DOAJ |
| description | Fruit bats and insectivorous bats are believed to provide a natural reservoir for a wide variety of infectious diseases. Several lines of evidence, including the successful isolation of infectious viruses, indicate that Marburg virus and Ravn virus have found a major reservoir in colonies of the Egyptian rousette (<em>Rousettus aegyptiacus</em>). To facilitate molecular studies on virus-reservoir host interactions and isolation of viruses from environmental samples, we established cell lines from primary cells of this animal. The cell lines were given to several laboratories until we realized that a contamination with Vero cells in one of the cultures had occurred. Here we describe a general diagnostic procedure for identification of cross-species contamination with the focus on Vero and <em>Rousettus</em> cell lines, and summarize newly discovered properties of the cell lines that may pertain to pathogen discovery. |
| first_indexed | 2024-12-13T04:14:02Z |
| format | Article |
| id | doaj.art-731bbe9a865646519272c79be35123c5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-13T04:14:02Z |
| publishDate | 2012-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-731bbe9a865646519272c79be35123c52022-12-21T23:59:55ZengMDPI AGViruses1999-49152012-05-014588990010.3390/v4050889Authentication of the R06E Fruit Bat Cell LineIngo JordanVolker SandigVincent J. MunsterFruit bats and insectivorous bats are believed to provide a natural reservoir for a wide variety of infectious diseases. Several lines of evidence, including the successful isolation of infectious viruses, indicate that Marburg virus and Ravn virus have found a major reservoir in colonies of the Egyptian rousette (<em>Rousettus aegyptiacus</em>). To facilitate molecular studies on virus-reservoir host interactions and isolation of viruses from environmental samples, we established cell lines from primary cells of this animal. The cell lines were given to several laboratories until we realized that a contamination with Vero cells in one of the cultures had occurred. Here we describe a general diagnostic procedure for identification of cross-species contamination with the focus on Vero and <em>Rousettus</em> cell lines, and summarize newly discovered properties of the cell lines that may pertain to pathogen discovery.http://www.mdpi.com/1999-4915/4/5/889fruit bat cell lineR06ER05T<em>Rousettus aegyptiacus</em> |
| spellingShingle | Ingo Jordan Volker Sandig Vincent J. Munster Authentication of the R06E Fruit Bat Cell Line Viruses fruit bat cell line R06E R05T <em>Rousettus aegyptiacus</em> |
| title | Authentication of the R06E Fruit Bat Cell Line |
| title_full | Authentication of the R06E Fruit Bat Cell Line |
| title_fullStr | Authentication of the R06E Fruit Bat Cell Line |
| title_full_unstemmed | Authentication of the R06E Fruit Bat Cell Line |
| title_short | Authentication of the R06E Fruit Bat Cell Line |
| title_sort | authentication of the r06e fruit bat cell line |
| topic | fruit bat cell line R06E R05T <em>Rousettus aegyptiacus</em> |
| url | http://www.mdpi.com/1999-4915/4/5/889 |
| work_keys_str_mv | AT ingojordan authenticationofther06efruitbatcellline AT volkersandig authenticationofther06efruitbatcellline AT vincentjmunster authenticationofther06efruitbatcellline |