Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential
Chondroitin sulfate (CS) is a type of glycosaminoglycan described as an antioxidant molecule that has been found in animal species such as fish. Tilapia (Oreochromis niloticus) represents an eco-friendly source of this compound, since its economical processing generates usable waste, reducing the ne...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
2021-07-01
|
| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021001000602&tlng=en |
| _version_ | 1798021427869253632 |
|---|---|
| author | L.H.C. Medeiros B.M.F. Vasconcelos M.B. Silva A.A. Souza-Junior S.F. Chavante G.P.V. Andrade |
| author_facet | L.H.C. Medeiros B.M.F. Vasconcelos M.B. Silva A.A. Souza-Junior S.F. Chavante G.P.V. Andrade |
| author_sort | L.H.C. Medeiros |
| collection | DOAJ |
| description | Chondroitin sulfate (CS) is a type of glycosaminoglycan described as an antioxidant molecule that has been found in animal species such as fish. Tilapia (Oreochromis niloticus) represents an eco-friendly source of this compound, since its economical processing generates usable waste, reducing the negative environmental impact. This waste was used for CS extraction, purification, characterization by enzymatic degradation, and evaluation of its antioxidant effect. CS obtained from tilapia presented sulfation mainly at carbon 4 of galactosamine, and it was not cytotoxic at concentrations up to 200 µg/mL. Furthermore, 100 µg/mL of CS from tilapia reduced the levels of reactive oxygen species to 47% of the total intracellular reactive oxygen species level. The ability of CS to chelate metal ions in vitro also suggested an ability to react with other pathways that generate oxidative radicals, such as the Haber-Weiss reaction, acting intracellularly in more than one way. Although the role of CS from tilapia remains unclear, the pharmacological effects described herein indicate that CS is a potential molecule for further study of the relationship between the structures and functions of chondroitin sulfates as antioxidants. |
| first_indexed | 2024-04-11T17:13:43Z |
| format | Article |
| id | doaj.art-731bc2d1ebaf4557b33620cdd6c7c551 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-11T17:13:43Z |
| publishDate | 2021-07-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-731bc2d1ebaf4557b33620cdd6c7c5512022-12-22T04:12:46ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2021-07-01541010.1590/1414-431x2020e10730Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potentialL.H.C. Medeiroshttps://orcid.org/0000-0002-0950-5748B.M.F. Vasconceloshttps://orcid.org/0000-0001-9494-5555M.B. Silvahttps://orcid.org/0000-0002-8277-8558A.A. Souza-Juniorhttps://orcid.org/0000-0001-6391-9418S.F. Chavantehttps://orcid.org/0000-0003-1825-3148G.P.V. Andradehttps://orcid.org/0000-0003-2805-9074Chondroitin sulfate (CS) is a type of glycosaminoglycan described as an antioxidant molecule that has been found in animal species such as fish. Tilapia (Oreochromis niloticus) represents an eco-friendly source of this compound, since its economical processing generates usable waste, reducing the negative environmental impact. This waste was used for CS extraction, purification, characterization by enzymatic degradation, and evaluation of its antioxidant effect. CS obtained from tilapia presented sulfation mainly at carbon 4 of galactosamine, and it was not cytotoxic at concentrations up to 200 µg/mL. Furthermore, 100 µg/mL of CS from tilapia reduced the levels of reactive oxygen species to 47% of the total intracellular reactive oxygen species level. The ability of CS to chelate metal ions in vitro also suggested an ability to react with other pathways that generate oxidative radicals, such as the Haber-Weiss reaction, acting intracellularly in more than one way. Although the role of CS from tilapia remains unclear, the pharmacological effects described herein indicate that CS is a potential molecule for further study of the relationship between the structures and functions of chondroitin sulfates as antioxidants.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021001000602&tlng=enGlycosaminoglycansChondroitin sulfateOreochromis niloticusAntioxidantReactive oxygen species |
| spellingShingle | L.H.C. Medeiros B.M.F. Vasconcelos M.B. Silva A.A. Souza-Junior S.F. Chavante G.P.V. Andrade Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential Brazilian Journal of Medical and Biological Research Glycosaminoglycans Chondroitin sulfate Oreochromis niloticus Antioxidant Reactive oxygen species |
| title | Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential |
| title_full | Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential |
| title_fullStr | Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential |
| title_full_unstemmed | Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential |
| title_short | Chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential |
| title_sort | chondroitin sulfate from fish waste exhibits strong intracellular antioxidant potential |
| topic | Glycosaminoglycans Chondroitin sulfate Oreochromis niloticus Antioxidant Reactive oxygen species |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021001000602&tlng=en |
| work_keys_str_mv | AT lhcmedeiros chondroitinsulfatefromfishwasteexhibitsstrongintracellularantioxidantpotential AT bmfvasconcelos chondroitinsulfatefromfishwasteexhibitsstrongintracellularantioxidantpotential AT mbsilva chondroitinsulfatefromfishwasteexhibitsstrongintracellularantioxidantpotential AT aasouzajunior chondroitinsulfatefromfishwasteexhibitsstrongintracellularantioxidantpotential AT sfchavante chondroitinsulfatefromfishwasteexhibitsstrongintracellularantioxidantpotential AT gpvandrade chondroitinsulfatefromfishwasteexhibitsstrongintracellularantioxidantpotential |