Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library
A new gene encoding a lipase (designated as Lip-1) was identified from a metagenomic bacterial artificial chromosome(BAC) library prepared from a concentrated water sample collected from a hot spring field in Niujie, Eryuan of Yunnan province in China. The open reading frame of this gene encoded 622...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X16301394 |
| _version_ | 1818966412841975808 |
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| author | Wei Yan Furong Li Li Wang Yaxin Zhu Zhiyang Dong Linhan Bai |
| author_facet | Wei Yan Furong Li Li Wang Yaxin Zhu Zhiyang Dong Linhan Bai |
| author_sort | Wei Yan |
| collection | DOAJ |
| description | A new gene encoding a lipase (designated as Lip-1) was identified from a metagenomic bacterial artificial chromosome(BAC) library prepared from a concentrated water sample collected from a hot spring field in Niujie, Eryuan of Yunnan province in China. The open reading frame of this gene encoded 622 amino acid residues. It was cloned, fused with the oleosin gene and over expressed in Escherichia coli to prepare immobilized lipase artificial oil body AOB-sole-lip-1. The monomeric Sole-lip-1 fusion protein presented a molecular mass of 102.4 kDa. Enzyme assays using olive oil and methanol as the substrates in petroleum ether confirmed its transesterification activity. Hexadecanoic acid methyl ester, 8,11-Octadecadienoic acid methyl ester, 8-Octadecenoic acid methyl ester, and Octadecanoic acid methyl ester were detected. It showed favorable transesterification activity with optimal temperature 45 °C. Besides, the maximal biodiesel yield was obtained when the petroleum ether system as the organic solvent and the substrate methanol in 350 mmol/L (at a molar ratio of methanol of 10.5:1) and the water content was 1%. In light of these advantages, this lipase presents a promising resource for biodiesel production. |
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| id | doaj.art-731c784d7ad940d59c82a160d4ab93c5 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-20T13:32:30Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
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| spelling | doaj.art-731c784d7ad940d59c82a160d4ab93c52022-12-21T19:39:03ZengElsevierBiotechnology Reports2215-017X2017-03-0114C273310.1016/j.btre.2016.12.007Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic libraryWei Yan0Furong Li1Li Wang2Yaxin Zhu3Zhiyang Dong4Linhan Bai5Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Science, Beijing, 100101, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Science, Beijing, 100101, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Science, Beijing, 100101, ChinaKey Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaA new gene encoding a lipase (designated as Lip-1) was identified from a metagenomic bacterial artificial chromosome(BAC) library prepared from a concentrated water sample collected from a hot spring field in Niujie, Eryuan of Yunnan province in China. The open reading frame of this gene encoded 622 amino acid residues. It was cloned, fused with the oleosin gene and over expressed in Escherichia coli to prepare immobilized lipase artificial oil body AOB-sole-lip-1. The monomeric Sole-lip-1 fusion protein presented a molecular mass of 102.4 kDa. Enzyme assays using olive oil and methanol as the substrates in petroleum ether confirmed its transesterification activity. Hexadecanoic acid methyl ester, 8,11-Octadecadienoic acid methyl ester, 8-Octadecenoic acid methyl ester, and Octadecanoic acid methyl ester were detected. It showed favorable transesterification activity with optimal temperature 45 °C. Besides, the maximal biodiesel yield was obtained when the petroleum ether system as the organic solvent and the substrate methanol in 350 mmol/L (at a molar ratio of methanol of 10.5:1) and the water content was 1%. In light of these advantages, this lipase presents a promising resource for biodiesel production.http://www.sciencedirect.com/science/article/pii/S2215017X16301394BAC libraryLipase activityNew genesImmobilized lipaseTransesterification |
| spellingShingle | Wei Yan Furong Li Li Wang Yaxin Zhu Zhiyang Dong Linhan Bai Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library Biotechnology Reports BAC library Lipase activity New genes Immobilized lipase Transesterification |
| title | Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library |
| title_full | Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library |
| title_fullStr | Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library |
| title_full_unstemmed | Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library |
| title_short | Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library |
| title_sort | discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library |
| topic | BAC library Lipase activity New genes Immobilized lipase Transesterification |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X16301394 |
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