Imaging Endocytosis Dynamics in Health and Disease
Endocytosis is a critical process for cell growth and viability. It mediates nutrient uptake, guarantees plasma membrane homeostasis, and generates intracellular signaling cascades. Moreover, it plays an important role in dead cell clearance and defense against external microbes. Finally, endocytosi...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Membranes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0375/12/4/393 |
| _version_ | 1827599903845515264 |
|---|---|
| author | Erica Tagliatti Katia Cortese |
| author_facet | Erica Tagliatti Katia Cortese |
| author_sort | Erica Tagliatti |
| collection | DOAJ |
| description | Endocytosis is a critical process for cell growth and viability. It mediates nutrient uptake, guarantees plasma membrane homeostasis, and generates intracellular signaling cascades. Moreover, it plays an important role in dead cell clearance and defense against external microbes. Finally, endocytosis is an important cellular route for the delivery of nanomedicines for therapeutic treatments. Thus, it is not surprising that both environmental and genetic perturbation of endocytosis have been associated with several human conditions such as cancer, neurological disorders, and virus infections, among others. Over the last decades, a lot of research has been focused on developing advanced imaging methods to monitor endocytosis events with high resolution in living cells and tissues. These include fluorescence imaging, electron microscopy, and correlative and super-resolution microscopy. In this review, we outline the major endocytic pathways and briefly discuss how defects in the molecular machinery of these pathways lead to disease. We then discuss the current imaging methodologies used to study endocytosis in different contexts, highlighting strengths and weaknesses. |
| first_indexed | 2024-03-09T04:24:54Z |
| format | Article |
| id | doaj.art-731c903130124abab160bc255c63346e |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T04:24:54Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-731c903130124abab160bc255c63346e2023-12-03T13:41:43ZengMDPI AGMembranes2077-03752022-04-0112439310.3390/membranes12040393Imaging Endocytosis Dynamics in Health and DiseaseErica Tagliatti0Katia Cortese1Laboratory of Pharmacology and Brain Pathology, Humanitas Clinical and Research Center, Via Manzoni 56, 20089 Milano, ItalyCellular Electron Microscopy Laboratory, Department of Experimental Medicine (DIMES), Human Anatomy, Università di Genova, Via Antonio de Toni 14, 16132 Genova, ItalyEndocytosis is a critical process for cell growth and viability. It mediates nutrient uptake, guarantees plasma membrane homeostasis, and generates intracellular signaling cascades. Moreover, it plays an important role in dead cell clearance and defense against external microbes. Finally, endocytosis is an important cellular route for the delivery of nanomedicines for therapeutic treatments. Thus, it is not surprising that both environmental and genetic perturbation of endocytosis have been associated with several human conditions such as cancer, neurological disorders, and virus infections, among others. Over the last decades, a lot of research has been focused on developing advanced imaging methods to monitor endocytosis events with high resolution in living cells and tissues. These include fluorescence imaging, electron microscopy, and correlative and super-resolution microscopy. In this review, we outline the major endocytic pathways and briefly discuss how defects in the molecular machinery of these pathways lead to disease. We then discuss the current imaging methodologies used to study endocytosis in different contexts, highlighting strengths and weaknesses.https://www.mdpi.com/2077-0375/12/4/393endocytosisprotein receptordiseasefluorescenceelectron microscopysuper-resolution microscopy |
| spellingShingle | Erica Tagliatti Katia Cortese Imaging Endocytosis Dynamics in Health and Disease Membranes endocytosis protein receptor disease fluorescence electron microscopy super-resolution microscopy |
| title | Imaging Endocytosis Dynamics in Health and Disease |
| title_full | Imaging Endocytosis Dynamics in Health and Disease |
| title_fullStr | Imaging Endocytosis Dynamics in Health and Disease |
| title_full_unstemmed | Imaging Endocytosis Dynamics in Health and Disease |
| title_short | Imaging Endocytosis Dynamics in Health and Disease |
| title_sort | imaging endocytosis dynamics in health and disease |
| topic | endocytosis protein receptor disease fluorescence electron microscopy super-resolution microscopy |
| url | https://www.mdpi.com/2077-0375/12/4/393 |
| work_keys_str_mv | AT ericatagliatti imagingendocytosisdynamicsinhealthanddisease AT katiacortese imagingendocytosisdynamicsinhealthanddisease |