Changes in Transcriptomic Profiles in Different Reproductive Periods in Yaks
Yak reproductive characteristics have received extensive attention, though the molecular regulation mechanism of its ovarian activity remains to be explored. Therefore, this study initially conducted a comparative analysis of yak ovarian activities in anestrus, estrus, and pregnancy regarding their...
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MDPI AG
2021-11-01
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| author | Shaoke Guo Mengli Cao Xingdong Wang Lin Xiong Xiaoyun Wu Pengjia Bao Min Chu Chunnian Liang Ping Yan Jie Pei Xian Guo |
| author_facet | Shaoke Guo Mengli Cao Xingdong Wang Lin Xiong Xiaoyun Wu Pengjia Bao Min Chu Chunnian Liang Ping Yan Jie Pei Xian Guo |
| author_sort | Shaoke Guo |
| collection | DOAJ |
| description | Yak reproductive characteristics have received extensive attention, though the molecular regulation mechanism of its ovarian activity remains to be explored. Therefore, this study initially conducted a comparative analysis of yak ovarian activities in anestrus, estrus, and pregnancy regarding their morphology and histology, followed by implementing RNA sequencing (RNA-seq) technology to detect the overall gene expression and biological mechanism in different reproductive stages. H&E staining showed that there were more growing follicles and mature follicles in ovarian tissue sections during estrus than ovarian tissues during non-estrus. The RNA-seq analysis of yak ovary tissues in three periods showed that DEGs related to follicular development and hormone metabolism were screened in the three comparison groups, such as <i>COL1A2</i>, <i>NR4A1</i>, <i>THBS2</i>, <i>PTGS2</i>, <i>SCARB1</i>, <i>STAR</i>, and <i>WNT2B</i>. Bioinformatics analysis showed that these DEGs are involved in ion binding, cell development, metabolic processes, enriched in ECM–receptor interactions, steroid biosynthesis, together with aldosterone generation/discharge and Wnt/PI3K-Akt signaling pathways. In addition, we speculate alternate splice development events to have important role/s in regulating ovarian functional genomic expression profiles. These results provide essential knowledge aimed at scrutinizing pivotal biomarkers for yak ovarian activity, together with paving the way for enhancing researchers’ focus on improving yak reproductive performance. |
| first_indexed | 2024-03-10T04:34:46Z |
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| id | doaj.art-7323e5abd8494c97be1e2aa19b113828 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T04:34:46Z |
| publishDate | 2021-11-01 |
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| series | Biology |
| spelling | doaj.art-7323e5abd8494c97be1e2aa19b1138282023-11-23T03:52:51ZengMDPI AGBiology2079-77372021-11-011012122910.3390/biology10121229Changes in Transcriptomic Profiles in Different Reproductive Periods in YaksShaoke Guo0Mengli Cao1Xingdong Wang2Lin Xiong3Xiaoyun Wu4Pengjia Bao5Min Chu6Chunnian Liang7Ping Yan8Jie Pei9Xian Guo10Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaYak reproductive characteristics have received extensive attention, though the molecular regulation mechanism of its ovarian activity remains to be explored. Therefore, this study initially conducted a comparative analysis of yak ovarian activities in anestrus, estrus, and pregnancy regarding their morphology and histology, followed by implementing RNA sequencing (RNA-seq) technology to detect the overall gene expression and biological mechanism in different reproductive stages. H&E staining showed that there were more growing follicles and mature follicles in ovarian tissue sections during estrus than ovarian tissues during non-estrus. The RNA-seq analysis of yak ovary tissues in three periods showed that DEGs related to follicular development and hormone metabolism were screened in the three comparison groups, such as <i>COL1A2</i>, <i>NR4A1</i>, <i>THBS2</i>, <i>PTGS2</i>, <i>SCARB1</i>, <i>STAR</i>, and <i>WNT2B</i>. Bioinformatics analysis showed that these DEGs are involved in ion binding, cell development, metabolic processes, enriched in ECM–receptor interactions, steroid biosynthesis, together with aldosterone generation/discharge and Wnt/PI3K-Akt signaling pathways. In addition, we speculate alternate splice development events to have important role/s in regulating ovarian functional genomic expression profiles. These results provide essential knowledge aimed at scrutinizing pivotal biomarkers for yak ovarian activity, together with paving the way for enhancing researchers’ focus on improving yak reproductive performance.https://www.mdpi.com/2079-7737/10/12/1229yakovaryanestrusestruspregnancy |
| spellingShingle | Shaoke Guo Mengli Cao Xingdong Wang Lin Xiong Xiaoyun Wu Pengjia Bao Min Chu Chunnian Liang Ping Yan Jie Pei Xian Guo Changes in Transcriptomic Profiles in Different Reproductive Periods in Yaks Biology yak ovary anestrus estrus pregnancy |
| title | Changes in Transcriptomic Profiles in Different Reproductive Periods in Yaks |
| title_full | Changes in Transcriptomic Profiles in Different Reproductive Periods in Yaks |
| title_fullStr | Changes in Transcriptomic Profiles in Different Reproductive Periods in Yaks |
| title_full_unstemmed | Changes in Transcriptomic Profiles in Different Reproductive Periods in Yaks |
| title_short | Changes in Transcriptomic Profiles in Different Reproductive Periods in Yaks |
| title_sort | changes in transcriptomic profiles in different reproductive periods in yaks |
| topic | yak ovary anestrus estrus pregnancy |
| url | https://www.mdpi.com/2079-7737/10/12/1229 |
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