Insulin like protein from camel milk and similarity with human insulin
From experimental studies, clinical studies and epidemiological studies it is proved beyond doubt that camel milk has potential role in prevention and treatment of diabetes. The main purpose of the study to isolate camel milk protein and compare its similarity with human insulin so that camel milk c...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2022-02-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/120933 |
| _version_ | 1811169059955277824 |
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| author | R P AGRAWAL R AGRAWAL M N RANGA M RIZWAN |
| author_facet | R P AGRAWAL R AGRAWAL M N RANGA M RIZWAN |
| author_sort | R P AGRAWAL |
| collection | DOAJ |
| description | From experimental studies, clinical studies and epidemiological studies it is proved beyond doubt that camel milk has potential role in prevention and treatment of diabetes. The main purpose of the study to isolate camel milk protein and compare its similarity with human insulin so that camel milk can be used as an adjunct therapy for diabetes. Raw camel milk (30 ml) was used for isolation of protein and peptides. The complete process included trypsin digestion, peptide fractionation and LC-MS technique. Digested and fractionized peptide sample was processed further for liquid chromatography and mass spectra/ tandem mass spectra were recorded in positive-ion and high sensitivity mode. MS/MS spectra were automatically calibrated during dynamic LC-MS. Raw data files were converted to Mascot Generic Format (MGF) and these MGF files were searched against UniPort, NCBI and common MS contaminant database. In our study 13 proteins and 22 peptide sequences were found similar to insulin/ insulin like growth factor and isoform. In our study some very large peptide sequence were identified which were seen similar to NUAK family SNF1-like kinase and this peptide sequence gives evidence of role of camel milk in cancer treatment. Observing so many similar peptides in camel milk sample with human insulin, isoform of insulin, receptors and others give strong evidence that camel milk have proteins/ peptides of such proteins similar to human insulin and give support to finding that camel milk contains insulin like molecule that mimics insulin interaction with its receptors. |
| first_indexed | 2024-04-10T16:35:42Z |
| format | Article |
| id | doaj.art-732487f3d9c349578b74a6135c84d9b4 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T16:35:42Z |
| publishDate | 2022-02-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-732487f3d9c349578b74a6135c84d9b42023-02-08T11:30:17ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272022-02-0192110.56093/ijans.v92i1.120933Insulin like protein from camel milk and similarity with human insulinR P AGRAWAL0R AGRAWAL1M N RANGA2M RIZWAN3S P Medical College, Bikaner, Rajasthan 334 001 IndiaS P Medical College, Bikaner, Rajasthan 334 001 IndiaS P Medical College, Bikaner, Rajasthan 334 001 IndiaS P Medical College, Bikaner, Rajasthan 334 001 IndiaFrom experimental studies, clinical studies and epidemiological studies it is proved beyond doubt that camel milk has potential role in prevention and treatment of diabetes. The main purpose of the study to isolate camel milk protein and compare its similarity with human insulin so that camel milk can be used as an adjunct therapy for diabetes. Raw camel milk (30 ml) was used for isolation of protein and peptides. The complete process included trypsin digestion, peptide fractionation and LC-MS technique. Digested and fractionized peptide sample was processed further for liquid chromatography and mass spectra/ tandem mass spectra were recorded in positive-ion and high sensitivity mode. MS/MS spectra were automatically calibrated during dynamic LC-MS. Raw data files were converted to Mascot Generic Format (MGF) and these MGF files were searched against UniPort, NCBI and common MS contaminant database. In our study 13 proteins and 22 peptide sequences were found similar to insulin/ insulin like growth factor and isoform. In our study some very large peptide sequence were identified which were seen similar to NUAK family SNF1-like kinase and this peptide sequence gives evidence of role of camel milk in cancer treatment. Observing so many similar peptides in camel milk sample with human insulin, isoform of insulin, receptors and others give strong evidence that camel milk have proteins/ peptides of such proteins similar to human insulin and give support to finding that camel milk contains insulin like molecule that mimics insulin interaction with its receptors.https://epubs.icar.org.in/index.php/IJAnS/article/view/120933Camel milkDiabetesILPLCMSPeptides |
| spellingShingle | R P AGRAWAL R AGRAWAL M N RANGA M RIZWAN Insulin like protein from camel milk and similarity with human insulin Indian Journal of Animal Sciences Camel milk Diabetes ILP LCMS Peptides |
| title | Insulin like protein from camel milk and similarity with human insulin |
| title_full | Insulin like protein from camel milk and similarity with human insulin |
| title_fullStr | Insulin like protein from camel milk and similarity with human insulin |
| title_full_unstemmed | Insulin like protein from camel milk and similarity with human insulin |
| title_short | Insulin like protein from camel milk and similarity with human insulin |
| title_sort | insulin like protein from camel milk and similarity with human insulin |
| topic | Camel milk Diabetes ILP LCMS Peptides |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/120933 |
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