A modified method for consistent and reliable Golgi-Cox staining in significantly reduced time
The two major limitations of Golgi-Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi-Cox method, in which the tissue blocks were maintained at 37 ± 10C during chromation for only 24 hours and consistent staining o...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2010-12-01
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| Series: | Frontiers in Neurology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fneur.2010.00157/full |
| _version_ | 1819065710437990400 |
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| author | Amit eRanjan Birendra N Mallick |
| author_facet | Amit eRanjan Birendra N Mallick |
| author_sort | Amit eRanjan |
| collection | DOAJ |
| description | The two major limitations of Golgi-Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi-Cox method, in which the tissue blocks were maintained at 37 ± 10C during chromation for only 24 hours and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive and provides uniform staining with very good resolution of neuronal soma, dendrites as well as spines. |
| first_indexed | 2024-12-21T15:50:47Z |
| format | Article |
| id | doaj.art-732c56da9e8f4e1288f15c4a68fec3a9 |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-12-21T15:50:47Z |
| publishDate | 2010-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-732c56da9e8f4e1288f15c4a68fec3a92022-12-21T18:58:14ZengFrontiers Media S.A.Frontiers in Neurology1664-22952010-12-01110.3389/fneur.2010.001576906A modified method for consistent and reliable Golgi-Cox staining in significantly reduced timeAmit eRanjan0Birendra N Mallick1Jawaharlal Nehru UniversityJawaharlal Nehru UniversityThe two major limitations of Golgi-Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi-Cox method, in which the tissue blocks were maintained at 37 ± 10C during chromation for only 24 hours and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive and provides uniform staining with very good resolution of neuronal soma, dendrites as well as spines.http://journal.frontiersin.org/Journal/10.3389/fneur.2010.00157/fullGolgi-Cox stainincubation temperaturenormal body temperaturereduced incubation periodthick sectionthick tissue block |
| spellingShingle | Amit eRanjan Birendra N Mallick A modified method for consistent and reliable Golgi-Cox staining in significantly reduced time Frontiers in Neurology Golgi-Cox stain incubation temperature normal body temperature reduced incubation period thick section thick tissue block |
| title | A modified method for consistent and reliable Golgi-Cox staining in significantly reduced time |
| title_full | A modified method for consistent and reliable Golgi-Cox staining in significantly reduced time |
| title_fullStr | A modified method for consistent and reliable Golgi-Cox staining in significantly reduced time |
| title_full_unstemmed | A modified method for consistent and reliable Golgi-Cox staining in significantly reduced time |
| title_short | A modified method for consistent and reliable Golgi-Cox staining in significantly reduced time |
| title_sort | modified method for consistent and reliable golgi cox staining in significantly reduced time |
| topic | Golgi-Cox stain incubation temperature normal body temperature reduced incubation period thick section thick tissue block |
| url | http://journal.frontiersin.org/Journal/10.3389/fneur.2010.00157/full |
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