A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics
The objective of this study was to evaluate the antibacterial mechanisms of phenolic acids as natural approaches against multi-drug resistant Escherichia coli (E. coli). For that purpose, five phenolic acids were combined with each other and 31 combinations were obtained in total. To select the most...
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| Format: | Article |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.738896/full |
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| author | Geyin Zhang Yunqiao Yang Fareed Uddin Memon Kaiyuan Hao Baichang Xu Shuaiyang Wang Ying Wang Enyun Wu Xiaogang Chen Wenguang Xiong Hongbin Si |
| author_facet | Geyin Zhang Yunqiao Yang Fareed Uddin Memon Kaiyuan Hao Baichang Xu Shuaiyang Wang Ying Wang Enyun Wu Xiaogang Chen Wenguang Xiong Hongbin Si |
| author_sort | Geyin Zhang |
| collection | DOAJ |
| description | The objective of this study was to evaluate the antibacterial mechanisms of phenolic acids as natural approaches against multi-drug resistant Escherichia coli (E. coli). For that purpose, five phenolic acids were combined with each other and 31 combinations were obtained in total. To select the most potent and effective combination, all of the obtained combinations were examined for minimum inhibitory concentration (MIC) and it was found that the compound phenolic acid (CPA) 19 (protocatechuic acid, hydrocinnamic acid, and chlorogenic acid at concentrations of 0.833, 0.208, and 1.677 mg/mL, respectively) showed better efficacy against E. coli compared to other combinations. Furthermore, based on tandem mass tag (TMT) proteomics, the treatment of CPA 19 significantly downregulated the proteins associated with resistance (Tsr, Tar, CheA, and CheW), OmpF, and FliC of multidrug-resistant E. coli. At the same time, we proved that CPA 19 improves the sensitivity of E. coli to antibiotics (ceftriaxone sodium, amoxicillin, fosfomycin, sulfamonomethoxine, gatifloxacin, lincomycin, florfenicol, cefotaxime sodium, and rifampicin), causes the flagellum to fall off, breaks the structure of the cell wall and cell membrane, and leads to macromolecules leaks from the cell. This evidence elaborated the potential therapeutic efficacy of CPA 19 and provided a significant contribution to the discovery of antibacterial agents. |
| first_indexed | 2024-12-14T09:02:31Z |
| format | Article |
| id | doaj.art-732e23e0f1d747b8b5f213f7fc70e661 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T09:02:31Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-732e23e0f1d747b8b5f213f7fc70e6612022-12-21T23:08:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.738896738896A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag ProteomicsGeyin Zhang0Yunqiao Yang1Fareed Uddin Memon2Kaiyuan Hao3Baichang Xu4Shuaiyang Wang5Ying Wang6Enyun Wu7Xiaogang Chen8Wenguang Xiong9Hongbin Si10College of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaThe objective of this study was to evaluate the antibacterial mechanisms of phenolic acids as natural approaches against multi-drug resistant Escherichia coli (E. coli). For that purpose, five phenolic acids were combined with each other and 31 combinations were obtained in total. To select the most potent and effective combination, all of the obtained combinations were examined for minimum inhibitory concentration (MIC) and it was found that the compound phenolic acid (CPA) 19 (protocatechuic acid, hydrocinnamic acid, and chlorogenic acid at concentrations of 0.833, 0.208, and 1.677 mg/mL, respectively) showed better efficacy against E. coli compared to other combinations. Furthermore, based on tandem mass tag (TMT) proteomics, the treatment of CPA 19 significantly downregulated the proteins associated with resistance (Tsr, Tar, CheA, and CheW), OmpF, and FliC of multidrug-resistant E. coli. At the same time, we proved that CPA 19 improves the sensitivity of E. coli to antibiotics (ceftriaxone sodium, amoxicillin, fosfomycin, sulfamonomethoxine, gatifloxacin, lincomycin, florfenicol, cefotaxime sodium, and rifampicin), causes the flagellum to fall off, breaks the structure of the cell wall and cell membrane, and leads to macromolecules leaks from the cell. This evidence elaborated the potential therapeutic efficacy of CPA 19 and provided a significant contribution to the discovery of antibacterial agents.https://www.frontiersin.org/articles/10.3389/fmicb.2021.738896/fullcompound phenolic acidsEscherichia coliTMTantibacterial mechanismnew antimicrobial agent |
| spellingShingle | Geyin Zhang Yunqiao Yang Fareed Uddin Memon Kaiyuan Hao Baichang Xu Shuaiyang Wang Ying Wang Enyun Wu Xiaogang Chen Wenguang Xiong Hongbin Si A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics Frontiers in Microbiology compound phenolic acids Escherichia coli TMT antibacterial mechanism new antimicrobial agent |
| title | A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics |
| title_full | A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics |
| title_fullStr | A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics |
| title_full_unstemmed | A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics |
| title_short | A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics |
| title_sort | natural antimicrobial agent analysis of antibacterial effect and mechanism of compound phenolic acid on escherichia coli based on tandem mass tag proteomics |
| topic | compound phenolic acids Escherichia coli TMT antibacterial mechanism new antimicrobial agent |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.738896/full |
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