Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei
Abstract Trichoderma reesei (T. reesei) is well-known for its excellent ability to secret a large quantity of cellulase. However, unlike the endogenous proteins, little is known about the molecular mechanisms governing heterologous protein production. Herein, we focused on the integration loci and t...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02046-w |
| _version_ | 1797863203576741888 |
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| author | Wangli Ji Xiaolu Wang Xiaoqing Liu Yuan Wang Fangui Liu Bo Xu Huiying Luo Tao Tu Wei Zhang Xinxin Xu Xiaoyun Su |
| author_facet | Wangli Ji Xiaolu Wang Xiaoqing Liu Yuan Wang Fangui Liu Bo Xu Huiying Luo Tao Tu Wei Zhang Xinxin Xu Xiaoyun Su |
| author_sort | Wangli Ji |
| collection | DOAJ |
| description | Abstract Trichoderma reesei (T. reesei) is well-known for its excellent ability to secret a large quantity of cellulase. However, unlike the endogenous proteins, little is known about the molecular mechanisms governing heterologous protein production. Herein, we focused on the integration loci and the secretory pathway, and investigated their combinatorial effects on heterologous gene expression in T. reesei using a glucose oxidase from Aspergillus niger as a model protein. Integration in the cel3c locus was more efficient than the cbh1 locus in expressing the AnGOx by increasing the transcription of AnGOx in the early stage. In addition, we discovered that interruption of the cel3c locus has an additional effect by increasing the expression of the secretory pathway component genes. Accordingly, overexpressing three secretory pathway component genes, that were snc1, sso2, and rho3, increased AnGOx expression in the cbh1 transformant but not in the cel3c transformant. |
| first_indexed | 2024-04-09T22:31:46Z |
| format | Article |
| id | doaj.art-732f3b5ec93b4778b451a79de1e6f879 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-09T22:31:46Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-732f3b5ec93b4778b451a79de1e6f8792023-03-22T12:43:24ZengBMCMicrobial Cell Factories1475-28592023-02-0122111110.1186/s12934-023-02046-wCombining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reeseiWangli Ji0Xiaolu Wang1Xiaoqing Liu2Yuan Wang3Fangui Liu4Bo Xu5Huiying Luo6Tao Tu7Wei Zhang8Xinxin Xu9Xiaoyun Su10Biotechnology Research Institute, Chinese Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesCollege of Biological and Food Engineering, Guangdong University of Petrochemical TechnologyCollege of Biological and Food Engineering, Guangdong University of Petrochemical TechnologyInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesAbstract Trichoderma reesei (T. reesei) is well-known for its excellent ability to secret a large quantity of cellulase. However, unlike the endogenous proteins, little is known about the molecular mechanisms governing heterologous protein production. Herein, we focused on the integration loci and the secretory pathway, and investigated their combinatorial effects on heterologous gene expression in T. reesei using a glucose oxidase from Aspergillus niger as a model protein. Integration in the cel3c locus was more efficient than the cbh1 locus in expressing the AnGOx by increasing the transcription of AnGOx in the early stage. In addition, we discovered that interruption of the cel3c locus has an additional effect by increasing the expression of the secretory pathway component genes. Accordingly, overexpressing three secretory pathway component genes, that were snc1, sso2, and rho3, increased AnGOx expression in the cbh1 transformant but not in the cel3c transformant.https://doi.org/10.1186/s12934-023-02046-wTrichoderma reeseiGlucose oxidaseAspergillus nigerIntegration lociSecretory pathway |
| spellingShingle | Wangli Ji Xiaolu Wang Xiaoqing Liu Yuan Wang Fangui Liu Bo Xu Huiying Luo Tao Tu Wei Zhang Xinxin Xu Xiaoyun Su Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei Microbial Cell Factories Trichoderma reesei Glucose oxidase Aspergillus niger Integration loci Secretory pathway |
| title | Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei |
| title_full | Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei |
| title_fullStr | Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei |
| title_full_unstemmed | Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei |
| title_short | Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei |
| title_sort | combining manipulation of integration loci and secretory pathway on expression of an aspergillus niger glucose oxidase gene in trichoderma reesei |
| topic | Trichoderma reesei Glucose oxidase Aspergillus niger Integration loci Secretory pathway |
| url | https://doi.org/10.1186/s12934-023-02046-w |
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