Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late Blight
Potato late blight, caused by <i>Phytophthora infestans</i>, leads to a significant reduction in the yield and value of potato. Biocontrol displays great potential in the suppression of plant diseases. Diallyl trisulfide (DATS) is a well-known natural compound for biocontrol, although th...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3474 |
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| author | Yongfei Jian Shun Feng Airong Huang Zhiming Zhu Jiaomei Zhang Shicai Tang Liang Jin Maozhi Ren Pan Dong |
| author_facet | Yongfei Jian Shun Feng Airong Huang Zhiming Zhu Jiaomei Zhang Shicai Tang Liang Jin Maozhi Ren Pan Dong |
| author_sort | Yongfei Jian |
| collection | DOAJ |
| description | Potato late blight, caused by <i>Phytophthora infestans</i>, leads to a significant reduction in the yield and value of potato. Biocontrol displays great potential in the suppression of plant diseases. Diallyl trisulfide (DATS) is a well-known natural compound for biocontrol, although there is little information about it against potato late blight. In this study, DATS was found to be able to inhibit the hyphae growth of <i>P. infestans</i>, reduce its pathogenicity on detached potato leaves and tubers, and induce the overall resistance of potato tubers. DATS significantly increases catalase (CAT) activity of potato tubers, and it does not affect the levels of peroxidase (POD), superoxide dismutase (SOD), and malondialdehyde (MDA). The transcriptome datasets show that totals of 607 and 60 significantly differentially expressed genes (DEGs) and miRNAs (DEMs) are detected. Twenty-one negatively regulated miRNA-mRNA interaction pairs are observed in the co-expression regulatory network, which are mainly enriched in metabolic pathways, biosynthesis of secondary metabolites, and starch and sucrose metabolism based on the KEGG pathway. Our observations provide new insight into the role of DATS in biocontrol of potato late blight. |
| first_indexed | 2024-03-11T08:43:00Z |
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| id | doaj.art-7330d26e89954bd2aa01c46b88de748f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:43:00Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7330d26e89954bd2aa01c46b88de748f2023-11-16T21:00:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244347410.3390/ijms24043474Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late BlightYongfei Jian0Shun Feng1Airong Huang2Zhiming Zhu3Jiaomei Zhang4Shicai Tang5Liang Jin6Maozhi Ren7Pan Dong8School of Life Sciences, Chongqing University, Chongqing 401331, ChinaSchool of Life Sciences, Chongqing University, Chongqing 401331, ChinaSchool of Life Sciences, Chongqing University, Chongqing 401331, ChinaSchool of Life Sciences, Chongqing University, Chongqing 401331, ChinaSchool of Life Sciences, Chongqing University, Chongqing 401331, ChinaSchool of Life Sciences, Chongqing University, Chongqing 401331, ChinaSchool of Life Sciences, Chongqing University, Chongqing 401331, ChinaSchool of Life Sciences, Chongqing University, Chongqing 401331, ChinaSchool of Life Sciences, Chongqing University, Chongqing 401331, ChinaPotato late blight, caused by <i>Phytophthora infestans</i>, leads to a significant reduction in the yield and value of potato. Biocontrol displays great potential in the suppression of plant diseases. Diallyl trisulfide (DATS) is a well-known natural compound for biocontrol, although there is little information about it against potato late blight. In this study, DATS was found to be able to inhibit the hyphae growth of <i>P. infestans</i>, reduce its pathogenicity on detached potato leaves and tubers, and induce the overall resistance of potato tubers. DATS significantly increases catalase (CAT) activity of potato tubers, and it does not affect the levels of peroxidase (POD), superoxide dismutase (SOD), and malondialdehyde (MDA). The transcriptome datasets show that totals of 607 and 60 significantly differentially expressed genes (DEGs) and miRNAs (DEMs) are detected. Twenty-one negatively regulated miRNA-mRNA interaction pairs are observed in the co-expression regulatory network, which are mainly enriched in metabolic pathways, biosynthesis of secondary metabolites, and starch and sucrose metabolism based on the KEGG pathway. Our observations provide new insight into the role of DATS in biocontrol of potato late blight.https://www.mdpi.com/1422-0067/24/4/3474oomycete disease<i>Phytophthora infestans</i>biocontroltranscriptome |
| spellingShingle | Yongfei Jian Shun Feng Airong Huang Zhiming Zhu Jiaomei Zhang Shicai Tang Liang Jin Maozhi Ren Pan Dong Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late Blight International Journal of Molecular Sciences oomycete disease <i>Phytophthora infestans</i> biocontrol transcriptome |
| title | Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late Blight |
| title_full | Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late Blight |
| title_fullStr | Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late Blight |
| title_full_unstemmed | Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late Blight |
| title_short | Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late Blight |
| title_sort | integrative mrna and microrna analysis exploring the inducing effect and mechanism of diallyl trisulfide dats on potato against late blight |
| topic | oomycete disease <i>Phytophthora infestans</i> biocontrol transcriptome |
| url | https://www.mdpi.com/1422-0067/24/4/3474 |
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