In vitro organogenesis in Sophora toromiro from nodal segments
Sophora toromiro is an arboreal endemic to Chile and extinct in its natural state, this specie has problem for its conservation due to its difficult propagation. The present study aimed to determine the organogenic response of Sophora toromiro nodal explants under in vitro conditions using different...
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2020-06-01
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| Series: | Biotecnología Vegetal |
| Subjects: | |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/666 |
| _version_ | 1818560061533847552 |
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| author | Santiago C. Vásquez Marlene Lorena Molina-Müller Peter Seemann Fahrenkrog Camilo Alexander Mestanza Uquillas Edwin Israel Villavicencio Sánchez |
| author_facet | Santiago C. Vásquez Marlene Lorena Molina-Müller Peter Seemann Fahrenkrog Camilo Alexander Mestanza Uquillas Edwin Israel Villavicencio Sánchez |
| author_sort | Santiago C. Vásquez |
| collection | DOAJ |
| description | Sophora toromiro is an arboreal endemic to Chile and extinct in its natural state, this specie has problem for its conservation due to its difficult propagation. The present study aimed to determine the organogenic response of Sophora toromiro nodal explants under in vitro conditions using different culture media and plant growth regulators. Nodal segments were used as explants, and responded satisfactorily to shoots induction when they were grown in MS or WPM medium with addition of 6-benzyl amino purine (BAP), α-naphthaleneacetic acid (NAA), indole 3-butyric acid (IBA) or indole-3-acetic acid (IAA). The highest shoots number (3.5 shoots / explant) was achieved when the explants grew in WPM medium + 0.1 mg l-1 IBA. For roots induction, the explants were transferred to new culture medium, where the highest rooting percentage (42.1 %) was achieved by explants cultivating in MS medium + 0.5 mg l-1 NAA. This study provides new information for S. toromiro propagation and conservation. |
| first_indexed | 2024-12-14T00:33:33Z |
| format | Article |
| id | doaj.art-7333cb4ad9fa494ea484034008c280a2 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-14T00:33:33Z |
| publishDate | 2020-06-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-7333cb4ad9fa494ea484034008c280a22022-12-21T23:24:45ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472020-06-01202147153In vitro organogenesis in Sophora toromiro from nodal segmentsSantiago C. Vásquez0https://orcid.org/0000-0002-3713-020XMarlene Lorena Molina-Müller 1https://orcid.org/0000-0003-2153-9294Peter Seemann Fahrenkrog2https://orcid.org/0000-0002-3315-8248Camilo Alexander Mestanza Uquillas3https://orcid.org/0000-0001-9299-170XEdwin Israel Villavicencio Sánchez4https://orcid.org/0000-0002-6476-489XUniversidad Nacional de Loja, Facultad Agropecuaria y de Recursos Naturales Renovables, Carrera de Agronomía. Loja. Loja. Ecuador.; Ecuador Universidad Nacional de Loja, Facultad Agropecuaria y de Recursos Naturales Renovables, Carrera de Agronomía. Loja. Loja. Ecuador.; Chile Universidad Austral de Chile, Facultad de Ciencias Agrarias, Instituto de Producción y Sanidad Vegetal. Valdivia. Valdivia. Chile.; ChileUniversidad Austral de Chile, Facultad de Ciencias Agrarias, Escuela de Graduados, Campus Isla Teja. Valdivia. Valdivia. Chile.; ChileUniversidad Nacional de Loja, Facultad Agropecuaria y de Recursos Naturales Renovables, Carrera de Agronomía. Loja. Loja. Ecuador.; Ecuador Sophora toromiro is an arboreal endemic to Chile and extinct in its natural state, this specie has problem for its conservation due to its difficult propagation. The present study aimed to determine the organogenic response of Sophora toromiro nodal explants under in vitro conditions using different culture media and plant growth regulators. Nodal segments were used as explants, and responded satisfactorily to shoots induction when they were grown in MS or WPM medium with addition of 6-benzyl amino purine (BAP), α-naphthaleneacetic acid (NAA), indole 3-butyric acid (IBA) or indole-3-acetic acid (IAA). The highest shoots number (3.5 shoots / explant) was achieved when the explants grew in WPM medium + 0.1 mg l-1 IBA. For roots induction, the explants were transferred to new culture medium, where the highest rooting percentage (42.1 %) was achieved by explants cultivating in MS medium + 0.5 mg l-1 NAA. This study provides new information for S. toromiro propagation and conservation.https://revista.ibp.co.cu/index.php/BV/article/view/666in vitro regenerationrootingshoot induction |
| spellingShingle | Santiago C. Vásquez Marlene Lorena Molina-Müller Peter Seemann Fahrenkrog Camilo Alexander Mestanza Uquillas Edwin Israel Villavicencio Sánchez In vitro organogenesis in Sophora toromiro from nodal segments Biotecnología Vegetal in vitro regeneration rooting shoot induction |
| title | In vitro organogenesis in Sophora toromiro from nodal segments |
| title_full | In vitro organogenesis in Sophora toromiro from nodal segments |
| title_fullStr | In vitro organogenesis in Sophora toromiro from nodal segments |
| title_full_unstemmed | In vitro organogenesis in Sophora toromiro from nodal segments |
| title_short | In vitro organogenesis in Sophora toromiro from nodal segments |
| title_sort | in vitro organogenesis in sophora toromiro from nodal segments |
| topic | in vitro regeneration rooting shoot induction |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/666 |
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