CircRNA_103809 Suppresses the Proliferation and Metastasis of Breast Cancer Cells by Sponging MicroRNA-532-3p (miR-532-3p)
Breast cancer has become one of the most serious disease threatening mankind health in the world. Accumulating studies indicated that circRNAs played an important role in the occurrence and progression of breast cancer, however, the roles of circRNA_103809 in breast cancer progression remain unclear...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2020.00485/full |
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| author | Minfeng Liu Can Luo Jianyu Dong Jingyun Guo Qing Luo Changsheng Ye Zhaoze Guo Zhaoze Guo |
| author_facet | Minfeng Liu Can Luo Jianyu Dong Jingyun Guo Qing Luo Changsheng Ye Zhaoze Guo Zhaoze Guo |
| author_sort | Minfeng Liu |
| collection | DOAJ |
| description | Breast cancer has become one of the most serious disease threatening mankind health in the world. Accumulating studies indicated that circRNAs played an important role in the occurrence and progression of breast cancer, however, the roles of circRNA_103809 in breast cancer progression remain unclear. Therefore, in this study, we aimed to clarify the potential role and regulatory mechanism of circRNA_103809 in the development of breast cancer. Firstly, the expression level of circRNA_103809 and microRNA-532-3p (miR-532-3p) in breast cancer tissues and normal tissues were detected with the quantitative real-time polymerase chain reaction (RT-qPCR). In addition, the cell proliferation ability, metastasis ability and related pathways were identified by Cell Counting Kit-8 (CCK-8), flow cytometry, and western blot, respectively. Furthermore, the connection between circRNA_103809 and miR-532-3p was detected by dual-luciferase reporter assay. Then, our data showed that circRNA_103809 was down-regulated in breast cancer tissues in contrast to adjacent non-tumor tissues, and the relative expression level of circRNA_103809 was closely associated with distant metastasis size, TNM stage, HER-2 status and overall survival time. In addition, our in vitro assays showed that the overexpression of circRNA_103809 could significantly inhibit epithelial-mesenchymal transition (EMT) pathway, then suppress breast cancer cell proliferation and metastasis ability. Moreover, we also found that the antitumor effect induced by circRNA_103809 could be reversed with the addition of miR-532-3p mimics. Taken together, this study showed that circRNA_103809 could inhibit cell proliferation and metastasis in breast cancer by sponging miR-532-3p, and circRNA_103809 might be a prospective target of breast cancer therapy. |
| first_indexed | 2024-12-17T10:52:26Z |
| format | Article |
| id | doaj.art-73360c8d94e941cfa9f4b1864c444e2c |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-17T10:52:26Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-73360c8d94e941cfa9f4b1864c444e2c2022-12-21T21:51:55ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-05-011110.3389/fgene.2020.00485529816CircRNA_103809 Suppresses the Proliferation and Metastasis of Breast Cancer Cells by Sponging MicroRNA-532-3p (miR-532-3p)Minfeng Liu0Can Luo1Jianyu Dong2Jingyun Guo3Qing Luo4Changsheng Ye5Zhaoze Guo6Zhaoze Guo7Department of Breast, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Breast, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Breast, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Breast, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Breast, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Breast, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Breast, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaThe First Clinical Medical College, Southern Medical University, Guangzhou, ChinaBreast cancer has become one of the most serious disease threatening mankind health in the world. Accumulating studies indicated that circRNAs played an important role in the occurrence and progression of breast cancer, however, the roles of circRNA_103809 in breast cancer progression remain unclear. Therefore, in this study, we aimed to clarify the potential role and regulatory mechanism of circRNA_103809 in the development of breast cancer. Firstly, the expression level of circRNA_103809 and microRNA-532-3p (miR-532-3p) in breast cancer tissues and normal tissues were detected with the quantitative real-time polymerase chain reaction (RT-qPCR). In addition, the cell proliferation ability, metastasis ability and related pathways were identified by Cell Counting Kit-8 (CCK-8), flow cytometry, and western blot, respectively. Furthermore, the connection between circRNA_103809 and miR-532-3p was detected by dual-luciferase reporter assay. Then, our data showed that circRNA_103809 was down-regulated in breast cancer tissues in contrast to adjacent non-tumor tissues, and the relative expression level of circRNA_103809 was closely associated with distant metastasis size, TNM stage, HER-2 status and overall survival time. In addition, our in vitro assays showed that the overexpression of circRNA_103809 could significantly inhibit epithelial-mesenchymal transition (EMT) pathway, then suppress breast cancer cell proliferation and metastasis ability. Moreover, we also found that the antitumor effect induced by circRNA_103809 could be reversed with the addition of miR-532-3p mimics. Taken together, this study showed that circRNA_103809 could inhibit cell proliferation and metastasis in breast cancer by sponging miR-532-3p, and circRNA_103809 might be a prospective target of breast cancer therapy.https://www.frontiersin.org/article/10.3389/fgene.2020.00485/fullcircRNA_103809miR-532-3pbreast cancerproliferationmetastasis |
| spellingShingle | Minfeng Liu Can Luo Jianyu Dong Jingyun Guo Qing Luo Changsheng Ye Zhaoze Guo Zhaoze Guo CircRNA_103809 Suppresses the Proliferation and Metastasis of Breast Cancer Cells by Sponging MicroRNA-532-3p (miR-532-3p) Frontiers in Genetics circRNA_103809 miR-532-3p breast cancer proliferation metastasis |
| title | CircRNA_103809 Suppresses the Proliferation and Metastasis of Breast Cancer Cells by Sponging MicroRNA-532-3p (miR-532-3p) |
| title_full | CircRNA_103809 Suppresses the Proliferation and Metastasis of Breast Cancer Cells by Sponging MicroRNA-532-3p (miR-532-3p) |
| title_fullStr | CircRNA_103809 Suppresses the Proliferation and Metastasis of Breast Cancer Cells by Sponging MicroRNA-532-3p (miR-532-3p) |
| title_full_unstemmed | CircRNA_103809 Suppresses the Proliferation and Metastasis of Breast Cancer Cells by Sponging MicroRNA-532-3p (miR-532-3p) |
| title_short | CircRNA_103809 Suppresses the Proliferation and Metastasis of Breast Cancer Cells by Sponging MicroRNA-532-3p (miR-532-3p) |
| title_sort | circrna 103809 suppresses the proliferation and metastasis of breast cancer cells by sponging microrna 532 3p mir 532 3p |
| topic | circRNA_103809 miR-532-3p breast cancer proliferation metastasis |
| url | https://www.frontiersin.org/article/10.3389/fgene.2020.00485/full |
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