Shiga Toxin-Producing <i>Escherichia coli</i> in Faecal Samples from Wild Ruminants
Wildlife can harbour Shiga toxin-producing <i>Escherichia coli</i> (STEC). In the present study, STEC in faecal samples from red deer (<i>n</i> = 106) and roe deer (<i>n</i> = 95) were characterised. All isolates were non-O157 strains. In red deer, STEC were detec...
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MDPI AG
2023-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/5/901 |
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| author | Anna Szczerba-Turek Filomena Chierchia Piotr Socha Wojciech Szweda |
| author_facet | Anna Szczerba-Turek Filomena Chierchia Piotr Socha Wojciech Szweda |
| author_sort | Anna Szczerba-Turek |
| collection | DOAJ |
| description | Wildlife can harbour Shiga toxin-producing <i>Escherichia coli</i> (STEC). In the present study, STEC in faecal samples from red deer (<i>n</i> = 106) and roe deer (<i>n</i> = 95) were characterised. All isolates were non-O157 strains. In red deer, STEC were detected in 17.9% (<i>n</i> = 19) of the isolates, and the <i>eae</i>/<i>stx</i><sub>2b</sub> virulence profile was detected in two isolates (10.5%). One STEC strain harboured <i>stx</i><sub>1a</sub> (5.3%) and eighteen STEC strains harboured <i>stx<sub>2</sub></i> (94.7%). The most prevalent <i>stx</i><sub>2</sub> subtypes were <i>stx</i><sub>2b</sub> (<i>n</i> = 12; 66.7%), <i>stx</i><sub>2a</sub> (<i>n</i> = 3; 16.7%), and <i>stx</i><sub>2g</sub> (<i>n</i> = 2; 11.1%). One isolate could not be subtyped (NS) with the applied primers (5.6%). The most widely identified serotypes were O146:H28 (<i>n</i> = 4; 21%), O146:HNM (<i>n</i> = 2; 10.5%), O103:H7 (<i>n</i> = 1; 5.3%), O103:H21 (<i>n</i> = 1; 5.3%), and O45:HNM (<i>n</i> = 1; 5.3%). In roe deer, STEC were detected in 16.8% (<i>n</i> = 16) of the isolates, and the <i>eae</i>/<i>stx</i><sub>2b</sub> virulence profile was detected in one isolate (6.3%). Two STEC strains harboured <i>stx</i><sub>1a</sub> (12.5%), one strain harboured <i>stx</i><sub>1NS</sub>/<i>stx</i><sub>2b</sub> (6.3%), and thirteen strains harboured <i>stx<sub>2</sub></i> (81.3%). The most common subtypes were <i>stx</i><sub>2b</sub> (<i>n</i> = 8; 61.5%), <i>stx</i><sub>2g</sub> (<i>n</i> = 2; 15.4%), non-typeable subtypes (NS) (<i>n</i> = 2; 15.4%), and <i>stx</i><sub>2a</sub> (<i>n</i> = 1; 7.7%). Serotype O146:H28 (<i>n</i> = 5; 31.3%) was identified. The study demonstrated that the zoonotic potential of STEC strains isolated from wildlife faeces should be monitored in the context of the ‘One Health’ approach which links human health with animal and environmental health. |
| first_indexed | 2024-03-11T07:31:52Z |
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| issn | 2076-2615 |
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| last_indexed | 2024-03-11T07:31:52Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-733b1ec7aca74040aacec2fbdb8c8b142023-11-17T07:13:52ZengMDPI AGAnimals2076-26152023-03-0113590110.3390/ani13050901Shiga Toxin-Producing <i>Escherichia coli</i> in Faecal Samples from Wild RuminantsAnna Szczerba-Turek0Filomena Chierchia1Piotr Socha2Wojciech Szweda3Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, PolandDepartment of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, PolandDepartment of Animal Reproduction with a Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, PolandDepartment of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, PolandWildlife can harbour Shiga toxin-producing <i>Escherichia coli</i> (STEC). In the present study, STEC in faecal samples from red deer (<i>n</i> = 106) and roe deer (<i>n</i> = 95) were characterised. All isolates were non-O157 strains. In red deer, STEC were detected in 17.9% (<i>n</i> = 19) of the isolates, and the <i>eae</i>/<i>stx</i><sub>2b</sub> virulence profile was detected in two isolates (10.5%). One STEC strain harboured <i>stx</i><sub>1a</sub> (5.3%) and eighteen STEC strains harboured <i>stx<sub>2</sub></i> (94.7%). The most prevalent <i>stx</i><sub>2</sub> subtypes were <i>stx</i><sub>2b</sub> (<i>n</i> = 12; 66.7%), <i>stx</i><sub>2a</sub> (<i>n</i> = 3; 16.7%), and <i>stx</i><sub>2g</sub> (<i>n</i> = 2; 11.1%). One isolate could not be subtyped (NS) with the applied primers (5.6%). The most widely identified serotypes were O146:H28 (<i>n</i> = 4; 21%), O146:HNM (<i>n</i> = 2; 10.5%), O103:H7 (<i>n</i> = 1; 5.3%), O103:H21 (<i>n</i> = 1; 5.3%), and O45:HNM (<i>n</i> = 1; 5.3%). In roe deer, STEC were detected in 16.8% (<i>n</i> = 16) of the isolates, and the <i>eae</i>/<i>stx</i><sub>2b</sub> virulence profile was detected in one isolate (6.3%). Two STEC strains harboured <i>stx</i><sub>1a</sub> (12.5%), one strain harboured <i>stx</i><sub>1NS</sub>/<i>stx</i><sub>2b</sub> (6.3%), and thirteen strains harboured <i>stx<sub>2</sub></i> (81.3%). The most common subtypes were <i>stx</i><sub>2b</sub> (<i>n</i> = 8; 61.5%), <i>stx</i><sub>2g</sub> (<i>n</i> = 2; 15.4%), non-typeable subtypes (NS) (<i>n</i> = 2; 15.4%), and <i>stx</i><sub>2a</sub> (<i>n</i> = 1; 7.7%). Serotype O146:H28 (<i>n</i> = 5; 31.3%) was identified. The study demonstrated that the zoonotic potential of STEC strains isolated from wildlife faeces should be monitored in the context of the ‘One Health’ approach which links human health with animal and environmental health.https://www.mdpi.com/2076-2615/13/5/901wildlifefoodborne diseaseSTEC‘One Health’infectious disease |
| spellingShingle | Anna Szczerba-Turek Filomena Chierchia Piotr Socha Wojciech Szweda Shiga Toxin-Producing <i>Escherichia coli</i> in Faecal Samples from Wild Ruminants Animals wildlife foodborne disease STEC ‘One Health’ infectious disease |
| title | Shiga Toxin-Producing <i>Escherichia coli</i> in Faecal Samples from Wild Ruminants |
| title_full | Shiga Toxin-Producing <i>Escherichia coli</i> in Faecal Samples from Wild Ruminants |
| title_fullStr | Shiga Toxin-Producing <i>Escherichia coli</i> in Faecal Samples from Wild Ruminants |
| title_full_unstemmed | Shiga Toxin-Producing <i>Escherichia coli</i> in Faecal Samples from Wild Ruminants |
| title_short | Shiga Toxin-Producing <i>Escherichia coli</i> in Faecal Samples from Wild Ruminants |
| title_sort | shiga toxin producing i escherichia coli i in faecal samples from wild ruminants |
| topic | wildlife foodborne disease STEC ‘One Health’ infectious disease |
| url | https://www.mdpi.com/2076-2615/13/5/901 |
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