Cytostatic activity of Geranium robertianum L. extracts processed by membrane procedures
In the present study the antioxidant and cytostatic capacities of some 8% Geranium robertianum (geranium) aqueous extracts processed by membrane procedures (micro- and ultrafiltration) were determined. The extracts were purified by microfiltration and then concentrated by successive ultrafiltrations...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-05-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535213003262 |
| _version_ | 1828168323360096256 |
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| author | Elena Neagu Gabriela Paun Daniel Constantin Gabriel Lucian Radu |
| author_facet | Elena Neagu Gabriela Paun Daniel Constantin Gabriel Lucian Radu |
| author_sort | Elena Neagu |
| collection | DOAJ |
| description | In the present study the antioxidant and cytostatic capacities of some 8% Geranium robertianum (geranium) aqueous extracts processed by membrane procedures (micro- and ultrafiltration) were determined. The extracts were purified by microfiltration and then concentrated by successive ultrafiltrations using Millipore membranes with 10,000 and 1000 Da cut-off. Two methods were used to establish the extracts’ antioxidant activity: DPPH and ABTS; the cytostatic capacity was evaluated on HEp-2p cell lines, by a qualitative method (cytochemical stain with Giemsa solution) and quantitative one (MTT test). Four concentrations of Geranium aqueous extracts were used to test cell viability: 100, 500, 1000 and 2000 μg/mL, 24 and 48 h, against the control culture (100% viability). The concentrated samples had the highest content of total polyphenols and the strongest antioxidant effect, having also the biggest impact upon cell viability. |
| first_indexed | 2024-04-12T02:32:09Z |
| format | Article |
| id | doaj.art-733c56ab14b64c5e9d73d13e55bcac05 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-12T02:32:09Z |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-733c56ab14b64c5e9d73d13e55bcac052022-12-22T03:51:44ZengElsevierArabian Journal of Chemistry1878-53522017-05-0110S2S2547S255310.1016/j.arabjc.2013.09.028Cytostatic activity of Geranium robertianum L. extracts processed by membrane proceduresElena Neagu0Gabriela Paun1Daniel Constantin2Gabriel Lucian Radu3Centre of Bioanalysis, National Institute for Research-Development of Biological Sciences, 296 Spl.Independentei, PO Box 17-16, 060031 Bucharest 6, RomaniaCentre of Bioanalysis, National Institute for Research-Development of Biological Sciences, 296 Spl.Independentei, PO Box 17-16, 060031 Bucharest 6, RomaniaCentre of Bioanalysis, National Institute for Research-Development of Biological Sciences, 296 Spl.Independentei, PO Box 17-16, 060031 Bucharest 6, RomaniaFaculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 313 Spl.Independentei, 060042 Bucharest, RomaniaIn the present study the antioxidant and cytostatic capacities of some 8% Geranium robertianum (geranium) aqueous extracts processed by membrane procedures (micro- and ultrafiltration) were determined. The extracts were purified by microfiltration and then concentrated by successive ultrafiltrations using Millipore membranes with 10,000 and 1000 Da cut-off. Two methods were used to establish the extracts’ antioxidant activity: DPPH and ABTS; the cytostatic capacity was evaluated on HEp-2p cell lines, by a qualitative method (cytochemical stain with Giemsa solution) and quantitative one (MTT test). Four concentrations of Geranium aqueous extracts were used to test cell viability: 100, 500, 1000 and 2000 μg/mL, 24 and 48 h, against the control culture (100% viability). The concentrated samples had the highest content of total polyphenols and the strongest antioxidant effect, having also the biggest impact upon cell viability.http://www.sciencedirect.com/science/article/pii/S1878535213003262Geranium robertianumUltrafiltrationCytostatic activityHEp-2p cellsPotential antitumor agents |
| spellingShingle | Elena Neagu Gabriela Paun Daniel Constantin Gabriel Lucian Radu Cytostatic activity of Geranium robertianum L. extracts processed by membrane procedures Arabian Journal of Chemistry Geranium robertianum Ultrafiltration Cytostatic activity HEp-2p cells Potential antitumor agents |
| title | Cytostatic activity of Geranium robertianum L. extracts processed by membrane procedures |
| title_full | Cytostatic activity of Geranium robertianum L. extracts processed by membrane procedures |
| title_fullStr | Cytostatic activity of Geranium robertianum L. extracts processed by membrane procedures |
| title_full_unstemmed | Cytostatic activity of Geranium robertianum L. extracts processed by membrane procedures |
| title_short | Cytostatic activity of Geranium robertianum L. extracts processed by membrane procedures |
| title_sort | cytostatic activity of geranium robertianum l extracts processed by membrane procedures |
| topic | Geranium robertianum Ultrafiltration Cytostatic activity HEp-2p cells Potential antitumor agents |
| url | http://www.sciencedirect.com/science/article/pii/S1878535213003262 |
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