Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.
Inflammation is a hallmark of several disease states ranging from neurodegeneration to sepsis but is also implicated in physiological processes like ageing. Non-resolving inflammation and prolonged neuroinflammation are unclear processes implicated in several conditions, including ageing. In this st...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4149545?pdf=render |
| _version_ | 1811223241968058368 |
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| author | Hui Qun Fu Ting Yang Wei Xiao Long Fan Yan Wu Niccolò Terrando Tian Long Wang |
| author_facet | Hui Qun Fu Ting Yang Wei Xiao Long Fan Yan Wu Niccolò Terrando Tian Long Wang |
| author_sort | Hui Qun Fu |
| collection | DOAJ |
| description | Inflammation is a hallmark of several disease states ranging from neurodegeneration to sepsis but is also implicated in physiological processes like ageing. Non-resolving inflammation and prolonged neuroinflammation are unclear processes implicated in several conditions, including ageing. In this study we studied the long-term effects of endotoxemia, as systemic lipopolysaccharide (LPS) injection, focusing on the role of astrocyte activation and cytokine release in the brain of aged rats. A single dose of LPS (2 mg/kg) or 0.9% saline was injected intraperitoneally in aged rats. Levels of pro-inflammatory cytokines (TNFα and IL-1β) and NF-κB p65 activation were measured systemically and in hippocampal tissue. Astrocytes and cytokines release in the CNS were detected via double immunofluorescence staining at different time-points up to day 30. Serum levels of TNFα and IL-1β were significantly increased acutely after 30 minutes (p<0.001) and up to 6 hours (p<0.001) following LPS-injection. Centrally, LPS-treated rats showed up-regulated mRNA expression and protein levels of pro-inflammatory cytokines in the hippocampus. These changes associated with astrogliosis in the hippocampus dentate gyrus (DG), IL-1β immunoreactivity and elevated NF-κB p65 expression up to day 30 post LPS exposure. Overall, these data demonstrate that LPS induces prolonged neuroinflammation and astrocyte activation in the hippocampus of aged rats. Hippocampal NF-κB p65 and excessive astrocytes-derived IL-1β release may play a pivotal role in regulating long-lasting neuroinflammation. |
| first_indexed | 2024-04-12T08:28:43Z |
| format | Article |
| id | doaj.art-73417f6283bb4918b64fd7d9e52836f7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T08:28:43Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-73417f6283bb4918b64fd7d9e52836f72022-12-22T03:40:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10633110.1371/journal.pone.0106331Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.Hui Qun FuTing YangWei XiaoLong FanYan WuNiccolò TerrandoTian Long WangInflammation is a hallmark of several disease states ranging from neurodegeneration to sepsis but is also implicated in physiological processes like ageing. Non-resolving inflammation and prolonged neuroinflammation are unclear processes implicated in several conditions, including ageing. In this study we studied the long-term effects of endotoxemia, as systemic lipopolysaccharide (LPS) injection, focusing on the role of astrocyte activation and cytokine release in the brain of aged rats. A single dose of LPS (2 mg/kg) or 0.9% saline was injected intraperitoneally in aged rats. Levels of pro-inflammatory cytokines (TNFα and IL-1β) and NF-κB p65 activation were measured systemically and in hippocampal tissue. Astrocytes and cytokines release in the CNS were detected via double immunofluorescence staining at different time-points up to day 30. Serum levels of TNFα and IL-1β were significantly increased acutely after 30 minutes (p<0.001) and up to 6 hours (p<0.001) following LPS-injection. Centrally, LPS-treated rats showed up-regulated mRNA expression and protein levels of pro-inflammatory cytokines in the hippocampus. These changes associated with astrogliosis in the hippocampus dentate gyrus (DG), IL-1β immunoreactivity and elevated NF-κB p65 expression up to day 30 post LPS exposure. Overall, these data demonstrate that LPS induces prolonged neuroinflammation and astrocyte activation in the hippocampus of aged rats. Hippocampal NF-κB p65 and excessive astrocytes-derived IL-1β release may play a pivotal role in regulating long-lasting neuroinflammation.http://europepmc.org/articles/PMC4149545?pdf=render |
| spellingShingle | Hui Qun Fu Ting Yang Wei Xiao Long Fan Yan Wu Niccolò Terrando Tian Long Wang Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. PLoS ONE |
| title | Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. |
| title_full | Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. |
| title_fullStr | Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. |
| title_full_unstemmed | Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. |
| title_short | Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. |
| title_sort | prolonged neuroinflammation after lipopolysaccharide exposure in aged rats |
| url | http://europepmc.org/articles/PMC4149545?pdf=render |
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