A Molecular Dynamic Model of Tryptophan Overproduction in <i>Escherichia coli</i>
Several deterministic models simulate the main molecular biology interactions among the numerous mechanisms controlling the dynamics of the tryptophan operon in native strains. However, no models exist to investigate bacterial tryptophan production from a biotechnological point of view. Here, we mod...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/10/560 |
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| author | Diego Andrés Castro-López Luis E. González de la Vara Moises Santillán Agustino Martínez-Antonio |
| author_facet | Diego Andrés Castro-López Luis E. González de la Vara Moises Santillán Agustino Martínez-Antonio |
| author_sort | Diego Andrés Castro-López |
| collection | DOAJ |
| description | Several deterministic models simulate the main molecular biology interactions among the numerous mechanisms controlling the dynamics of the tryptophan operon in native strains. However, no models exist to investigate bacterial tryptophan production from a biotechnological point of view. Here, we modified tryptophan models for native production to propose a biotechnological working model that incorporates the activity of tryptophan secretion systems and genetic modifications made in two reported <i>E. coli</i> strains. The resultant deterministic model could emulate the production of tryptophan in the same order of magnitude as those quantified experimentally by the genetically engineered <i>E. coli</i> strains GPT1001 and GPT1002 in shake flasks. We hope this work may contribute to the rational development of biological models that define and include the main parameters and molecular components for designing and engineering efficient biotechnological chassis to produce valuable chemicals. |
| first_indexed | 2024-03-09T09:46:56Z |
| format | Article |
| id | doaj.art-73447ca683444322bc62f4b642a2e848 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T09:46:56Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-73447ca683444322bc62f4b642a2e8482023-12-02T00:28:39ZengMDPI AGFermentation2311-56372022-10-0181056010.3390/fermentation8100560A Molecular Dynamic Model of Tryptophan Overproduction in <i>Escherichia coli</i>Diego Andrés Castro-López0Luis E. González de la Vara1Moises Santillán2Agustino Martínez-Antonio3Laboratorio de Ingeniería Biológica, Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-Unidad Irapuato), Km 9.6 Carr. Irapuato-León, Irapuato 36824, Gto, MexicoLaboratorio de Bioenergética y Biomembranas, Departamento de Bioquímica y Biotecnología, Cinvestav Irapuato, Irapuato 36824, Gto, MexicoCinvestav Unidad Monterrey, Vía del Conocimiento 201, Parque de Investigación e Innovación Tecnológica, Apodaca 66600, NL, MexicoLaboratorio de Ingeniería Biológica, Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-Unidad Irapuato), Km 9.6 Carr. Irapuato-León, Irapuato 36824, Gto, MexicoSeveral deterministic models simulate the main molecular biology interactions among the numerous mechanisms controlling the dynamics of the tryptophan operon in native strains. However, no models exist to investigate bacterial tryptophan production from a biotechnological point of view. Here, we modified tryptophan models for native production to propose a biotechnological working model that incorporates the activity of tryptophan secretion systems and genetic modifications made in two reported <i>E. coli</i> strains. The resultant deterministic model could emulate the production of tryptophan in the same order of magnitude as those quantified experimentally by the genetically engineered <i>E. coli</i> strains GPT1001 and GPT1002 in shake flasks. We hope this work may contribute to the rational development of biological models that define and include the main parameters and molecular components for designing and engineering efficient biotechnological chassis to produce valuable chemicals.https://www.mdpi.com/2311-5637/8/10/560dynamical modeltryptophan productionbiotechnology simulation<i>Escherichia coli</i>experimental data fit |
| spellingShingle | Diego Andrés Castro-López Luis E. González de la Vara Moises Santillán Agustino Martínez-Antonio A Molecular Dynamic Model of Tryptophan Overproduction in <i>Escherichia coli</i> Fermentation dynamical model tryptophan production biotechnology simulation <i>Escherichia coli</i> experimental data fit |
| title | A Molecular Dynamic Model of Tryptophan Overproduction in <i>Escherichia coli</i> |
| title_full | A Molecular Dynamic Model of Tryptophan Overproduction in <i>Escherichia coli</i> |
| title_fullStr | A Molecular Dynamic Model of Tryptophan Overproduction in <i>Escherichia coli</i> |
| title_full_unstemmed | A Molecular Dynamic Model of Tryptophan Overproduction in <i>Escherichia coli</i> |
| title_short | A Molecular Dynamic Model of Tryptophan Overproduction in <i>Escherichia coli</i> |
| title_sort | molecular dynamic model of tryptophan overproduction in i escherichia coli i |
| topic | dynamical model tryptophan production biotechnology simulation <i>Escherichia coli</i> experimental data fit |
| url | https://www.mdpi.com/2311-5637/8/10/560 |
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