Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors
Introduction Composite materials used in dentistry indicate adverse biological effects in laboratory conditions. One reason for this activity is incomplete conversion of their polymer matrix, favoring chemical instability and release of biologically harmful components to the external environment. A...
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| Format: | Article |
| Language: | English |
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Institute of Rural Health
2017-12-01
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| Series: | Annals of Agricultural and Environmental Medicine |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/aaem/Uncompleted-polymerization-and-cytotoxicity-of-dental-restorative-materials-as-potential-nhealth-risk-factors,72537,0,2.html |
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| author | Konrad Małkiewicz Piotr Wychowański Joanna Olkowska-Truchanowicz Marzena Tykarska Michał Czerwiński Marcin Wilczko Alfred Owoc |
| author_facet | Konrad Małkiewicz Piotr Wychowański Joanna Olkowska-Truchanowicz Marzena Tykarska Michał Czerwiński Marcin Wilczko Alfred Owoc |
| author_sort | Konrad Małkiewicz |
| collection | DOAJ |
| description | Introduction
Composite materials used in dentistry indicate adverse biological effects in laboratory conditions. One reason for this activity is incomplete conversion of their polymer matrix, favoring chemical instability and release of biologically harmful components to the external environment.
Aim
The aim of the study was to assess the degree of conversion of restorative materials commonly available on the European market and to examine the cytotoxic effects of their eluates in vitro.
Material and Methods
Using the Fournier transform infrared spectroscopy (FTIR) technique of analysis, the degree of polymer matrix conversion of 6 restorative materials was examined: Gradia Direct, Arkon, Filtek Z550, Herculite XRV, Tetric Evo Ceram, Charisma, polymerized with LED light. In order to assess the cytotoxicity of eluates of the studied materials obtained after 1 hour , 24 hours and 7 days, the MTT assay was used in cultured 3T3 cells. The results were statistically analyzed at significance level of p=0.05.
Results
The conversion degree of the assessed polymers ranged from 31.56% for Tetric Evo Ceram to 75.84% for Arcon. The strongest (p=0.05) cytotoxic effect was demonstrated after 7-day observation of Tetric Evo Ceram eluates, reducing the metabolic activity of cells down to 56%. A positive correlation (r(x, y) = 0.62) between the degree of conversion of composite materials and cytotoxic effects of their eluates on cell cultures was confirmed.
Conclusions
1. Restorative dental materials are chemically unstable in the conditions of the present study.
2. Polymer-based restorative dental materials available on the European market demonstrate cytotoxic properties constituting a potential threat to the patients’ health. |
| first_indexed | 2024-04-12T00:40:30Z |
| format | Article |
| id | doaj.art-7344b4946e024e4498c125d1a5ad0ef2 |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-04-12T00:40:30Z |
| publishDate | 2017-12-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-7344b4946e024e4498c125d1a5ad0ef22022-12-22T03:55:01ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632017-12-0124461862310.5604/12321966.123515972537Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factorsKonrad Małkiewicz0Piotr Wychowański1Joanna Olkowska-Truchanowicz2Marzena Tykarska3Michał Czerwiński4Marcin Wilczko5Alfred Owoc6Department of Orthodontics, Medical University of Warsaw, PolandDepartment of Oral Surgery, Medical University of Warsaw, PolandDepartment of Transplantology and Central Tissue Bank, Centre of Biostructure Research, Medical University of Warsaw, PolandInstitute of Chemistry, Faculty of Advanced Technologies and Chemistry, Military University of Technology, Warsaw, PolandInstitute of Chemistry, Faculty of Advanced Technologies and Chemistry, Military University of Technology, Warsaw, PolandPrivate Practice, Białystok, PolandCenter for Public Health and Health Promotion, Institute of Rural Health in Lublin, PolandIntroduction Composite materials used in dentistry indicate adverse biological effects in laboratory conditions. One reason for this activity is incomplete conversion of their polymer matrix, favoring chemical instability and release of biologically harmful components to the external environment. Aim The aim of the study was to assess the degree of conversion of restorative materials commonly available on the European market and to examine the cytotoxic effects of their eluates in vitro. Material and Methods Using the Fournier transform infrared spectroscopy (FTIR) technique of analysis, the degree of polymer matrix conversion of 6 restorative materials was examined: Gradia Direct, Arkon, Filtek Z550, Herculite XRV, Tetric Evo Ceram, Charisma, polymerized with LED light. In order to assess the cytotoxicity of eluates of the studied materials obtained after 1 hour , 24 hours and 7 days, the MTT assay was used in cultured 3T3 cells. The results were statistically analyzed at significance level of p=0.05. Results The conversion degree of the assessed polymers ranged from 31.56% for Tetric Evo Ceram to 75.84% for Arcon. The strongest (p=0.05) cytotoxic effect was demonstrated after 7-day observation of Tetric Evo Ceram eluates, reducing the metabolic activity of cells down to 56%. A positive correlation (r(x, y) = 0.62) between the degree of conversion of composite materials and cytotoxic effects of their eluates on cell cultures was confirmed. Conclusions 1. Restorative dental materials are chemically unstable in the conditions of the present study. 2. Polymer-based restorative dental materials available on the European market demonstrate cytotoxic properties constituting a potential threat to the patients’ health.http://www.journalssystem.com/aaem/Uncompleted-polymerization-and-cytotoxicity-of-dental-restorative-materials-as-potential-nhealth-risk-factors,72537,0,2.htmldental compositescytotoxicityDegree of Conversion |
| spellingShingle | Konrad Małkiewicz Piotr Wychowański Joanna Olkowska-Truchanowicz Marzena Tykarska Michał Czerwiński Marcin Wilczko Alfred Owoc Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors Annals of Agricultural and Environmental Medicine dental composites cytotoxicity Degree of Conversion |
| title | Uncompleted polymerization and cytotoxicity of dental restorative materials as potential
health risk factors |
| title_full | Uncompleted polymerization and cytotoxicity of dental restorative materials as potential
health risk factors |
| title_fullStr | Uncompleted polymerization and cytotoxicity of dental restorative materials as potential
health risk factors |
| title_full_unstemmed | Uncompleted polymerization and cytotoxicity of dental restorative materials as potential
health risk factors |
| title_short | Uncompleted polymerization and cytotoxicity of dental restorative materials as potential
health risk factors |
| title_sort | uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors |
| topic | dental composites cytotoxicity Degree of Conversion |
| url | http://www.journalssystem.com/aaem/Uncompleted-polymerization-and-cytotoxicity-of-dental-restorative-materials-as-potential-nhealth-risk-factors,72537,0,2.html |
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