Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay
Summary Early detection of expanded‐spectrum cephalosporin (ESC) resistance is essential not only for an effective therapy but also for the prompt implementation of infection control measures to prevent dissemination in the hospital. We have developed and validated a lateral flow immunoassay (LFIA),...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-02-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13892 |
| _version_ | 1819281596653502464 |
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| author | Christian Moguet Camille Gonzalez Antoine Sallustrau Stéphanie Gelhaye Thierry Naas Stéphanie Simon Hervé Volland |
| author_facet | Christian Moguet Camille Gonzalez Antoine Sallustrau Stéphanie Gelhaye Thierry Naas Stéphanie Simon Hervé Volland |
| author_sort | Christian Moguet |
| collection | DOAJ |
| description | Summary Early detection of expanded‐spectrum cephalosporin (ESC) resistance is essential not only for an effective therapy but also for the prompt implementation of infection control measures to prevent dissemination in the hospital. We have developed and validated a lateral flow immunoassay (LFIA), called LFIA‐CTX test, for the detection of ESC (cefotaxime) hydrolytic activity based on structural discrimination between the intact antibiotic and its hydrolysed product. A single bacterial colony was suspended in an extraction buffer containing cefotaxime. After a 30‐min incubation, the solution is loaded on the LFIA for reading within 10 min. A total of 348 well‐characterized Gram‐negative isolates were tested. Among them, the 38 isolates that did not express any cefotaxime‐hydrolysing β‐lactamase gave negative results. Of the 310 isolates expressing at least one cefotaxime‐hydrolysing β‐lactamase, all were tested positive, except three OXA‐48‐like producers, which were repeatedly detected negative. Therefore, the sensitivity was 99.1% and the specificity was 100%. The LFIA‐CTX test is efficient, fast, low‐cost and easy to implement in the workflow of a routine microbiology laboratory. |
| first_indexed | 2024-12-24T01:02:13Z |
| format | Article |
| id | doaj.art-73462f1e796c4c2b9be4f61c37d2c96e |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-24T01:02:13Z |
| publishDate | 2022-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-73462f1e796c4c2b9be4f61c37d2c96e2022-12-21T17:23:20ZengWileyMicrobial Biotechnology1751-79152022-02-0115260361210.1111/1751-7915.13892Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassayChristian Moguet0Camille Gonzalez1Antoine Sallustrau2Stéphanie Gelhaye3Thierry Naas4Stéphanie Simon5Hervé Volland6Département Médicaments et Technologies pour la Santé (DMTS) SPI Université Paris‐Saclay CEA, INRAE Gif‐sur‐Yvette 91191 FranceBacteriology‐Hygiene Unit APHP, Hôpital Bicêtre Le Kremlin‐Bicêtre FranceDépartement Médicaments et Technologies pour la Santé (DMTS) SCBM Université Paris‐Saclay CEA, INRAE Gif‐sur‐Yvette 91191 FranceDépartement Médicaments et Technologies pour la Santé (DMTS) SPI Université Paris‐Saclay CEA, INRAE Gif‐sur‐Yvette 91191 FranceBacteriology‐Hygiene Unit APHP, Hôpital Bicêtre Le Kremlin‐Bicêtre FranceDépartement Médicaments et Technologies pour la Santé (DMTS) SPI Université Paris‐Saclay CEA, INRAE Gif‐sur‐Yvette 91191 FranceDépartement Médicaments et Technologies pour la Santé (DMTS) SPI Université Paris‐Saclay CEA, INRAE Gif‐sur‐Yvette 91191 FranceSummary Early detection of expanded‐spectrum cephalosporin (ESC) resistance is essential not only for an effective therapy but also for the prompt implementation of infection control measures to prevent dissemination in the hospital. We have developed and validated a lateral flow immunoassay (LFIA), called LFIA‐CTX test, for the detection of ESC (cefotaxime) hydrolytic activity based on structural discrimination between the intact antibiotic and its hydrolysed product. A single bacterial colony was suspended in an extraction buffer containing cefotaxime. After a 30‐min incubation, the solution is loaded on the LFIA for reading within 10 min. A total of 348 well‐characterized Gram‐negative isolates were tested. Among them, the 38 isolates that did not express any cefotaxime‐hydrolysing β‐lactamase gave negative results. Of the 310 isolates expressing at least one cefotaxime‐hydrolysing β‐lactamase, all were tested positive, except three OXA‐48‐like producers, which were repeatedly detected negative. Therefore, the sensitivity was 99.1% and the specificity was 100%. The LFIA‐CTX test is efficient, fast, low‐cost and easy to implement in the workflow of a routine microbiology laboratory.https://doi.org/10.1111/1751-7915.13892 |
| spellingShingle | Christian Moguet Camille Gonzalez Antoine Sallustrau Stéphanie Gelhaye Thierry Naas Stéphanie Simon Hervé Volland Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay Microbial Biotechnology |
| title | Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay |
| title_full | Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay |
| title_fullStr | Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay |
| title_full_unstemmed | Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay |
| title_short | Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay |
| title_sort | detection of expanded spectrum cephalosporin hydrolysis by lateral flow immunoassay |
| url | https://doi.org/10.1111/1751-7915.13892 |
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