Identification of Gene Resistance to Avian InfluenzaVirus (Mx Gene) among Wild Waterbirds
The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AIvirus subty...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2013-04-01
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| Series: | Makara Seri Sains |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/index.php/science/article/view/1628 |
| _version_ | 1818381223369637888 |
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| author | Dewi Elfidasari Dedy Duryadi Solihin Retno Damayanti Soejoedono Sri Murtini |
| author_facet | Dewi Elfidasari Dedy Duryadi Solihin Retno Damayanti Soejoedono Sri Murtini |
| author_sort | Dewi Elfidasari |
| collection | DOAJ |
| description | The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AIvirus subtype H5N1, whereas the Mx-gene shows signs of susceptible. The objective of thisresearch was to detect the Mxgene in wild aquatic birds using the Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mxgene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mxgene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens. |
| first_indexed | 2024-12-14T02:31:10Z |
| format | Article |
| id | doaj.art-73497d367a85473eb343e65cf92f4072 |
| institution | Directory Open Access Journal |
| issn | 1693-6671 |
| language | English |
| last_indexed | 2024-12-14T02:31:10Z |
| publishDate | 2013-04-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Seri Sains |
| spelling | doaj.art-73497d367a85473eb343e65cf92f40722022-12-21T23:20:15ZengUniversitas IndonesiaMakara Seri Sains1693-66712013-04-01171610Identification of Gene Resistance to Avian InfluenzaVirus (Mx Gene) among Wild WaterbirdsDewi ElfidasariDedy Duryadi SolihinRetno Damayanti SoejoedonoSri MurtiniThe Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AIvirus subtype H5N1, whereas the Mx-gene shows signs of susceptible. The objective of thisresearch was to detect the Mxgene in wild aquatic birds using the Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mxgene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mxgene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens.http://journal.ui.ac.id/index.php/science/article/view/1628Mx genewild waterbirdsexposureAI virus subtype H5N1resistance |
| spellingShingle | Dewi Elfidasari Dedy Duryadi Solihin Retno Damayanti Soejoedono Sri Murtini Identification of Gene Resistance to Avian InfluenzaVirus (Mx Gene) among Wild Waterbirds Makara Seri Sains Mx gene wild waterbirds exposure AI virus subtype H5N1 resistance |
| title | Identification of Gene Resistance to Avian InfluenzaVirus (Mx Gene) among Wild Waterbirds |
| title_full | Identification of Gene Resistance to Avian InfluenzaVirus (Mx Gene) among Wild Waterbirds |
| title_fullStr | Identification of Gene Resistance to Avian InfluenzaVirus (Mx Gene) among Wild Waterbirds |
| title_full_unstemmed | Identification of Gene Resistance to Avian InfluenzaVirus (Mx Gene) among Wild Waterbirds |
| title_short | Identification of Gene Resistance to Avian InfluenzaVirus (Mx Gene) among Wild Waterbirds |
| title_sort | identification of gene resistance to avian influenzavirus mx gene among wild waterbirds |
| topic | Mx gene wild waterbirds exposure AI virus subtype H5N1 resistance |
| url | http://journal.ui.ac.id/index.php/science/article/view/1628 |
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