Genome‐wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Callose is a β‐1,3‐glucan commonly found in higher plants that plays an important role in regulating plant pollen development. It is synthesized by glucan synthase‐like (GSL) and is degraded by the enzyme endo‐1,3‐β‐glucosidase. However, genome‐wide analyses of callose GSL and endo‐1,3‐β‐glucosidase...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-08-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12685 |
| _version_ | 1797667844167565312 |
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| author | Yanan Pu Lingyun Hou Yingqi Guo Ikram Ullah Yongping Yang Yanling Yue |
| author_facet | Yanan Pu Lingyun Hou Yingqi Guo Ikram Ullah Yongping Yang Yanling Yue |
| author_sort | Yanan Pu |
| collection | DOAJ |
| description | Callose is a β‐1,3‐glucan commonly found in higher plants that plays an important role in regulating plant pollen development. It is synthesized by glucan synthase‐like (GSL) and is degraded by the enzyme endo‐1,3‐β‐glucosidase. However, genome‐wide analyses of callose GSL and endo‐1,3‐β‐glucosidase enzymes in fertile and sterile flower buds of Chinese cabbage have not yet been reported. Here, we show that delayed callose degradation at the tetrad stage may be the main cause of microspore abortion in Chinese cabbage with nuclear sterility near‐isogenic line ‘10L03’. Fifteen callose GSLs and 77 endo‐1,3‐β‐glucosidase enzymes were identified in Chinese cabbage. Phylogenetic, gene structural and chromosomal analyses revealed that the expansion occurred due to three polyploidization events of these two gene families. Expression pattern analysis showed that the GSL and endo‐1,3‐β‐glucosidase enzymes are involved in the development of various tissues and that the genes functionally diverged during long‐term evolution. Relative gene expression analysis of Chinese cabbage flowers at different developmental stages showed that high expression of the synthetic enzyme BraA01g041620 and low expression of AtA6‐homologous genes (BraA04g008040, BraA07g009320, BraA01g030220 and BraA03g040850) and two other genes (BraA10g020080 and BraA05g038340) for degrading enzymes in the meiosis and tetrad stages may cause nuclear sterility in the near‐isogenic line ‘10L03’. Overall, our data provide an important foundation for comprehending the potential roles of the callose GSL and endo‐1,3‐β‐glucosidase enzymes in regulating pollen development in Chinese cabbage. |
| first_indexed | 2024-03-11T20:19:27Z |
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| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-11T20:19:27Z |
| publishDate | 2019-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-734dfd1498c34f8eb74ce66981d082b02023-10-03T08:11:33ZengWileyFEBS Open Bio2211-54632019-08-01981432144910.1002/2211-5463.12685Genome‐wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis)Yanan Pu0Lingyun Hou1Yingqi Guo2Ikram Ullah3Yongping Yang4Yanling Yue5College of Landscape and Horticulture Yunnan Agricultural University Kunming ChinaCollege of Landscape and Horticulture Yunnan Agricultural University Kunming ChinaKunming Institute of Zoology Chinese Academy of Sciences Kunming ChinaCollege of Landscape and Horticulture Yunnan Agricultural University Kunming ChinaKunming Institute of Botany Chinese Academy of Sciences Kunming ChinaCollege of Landscape and Horticulture Yunnan Agricultural University Kunming ChinaCallose is a β‐1,3‐glucan commonly found in higher plants that plays an important role in regulating plant pollen development. It is synthesized by glucan synthase‐like (GSL) and is degraded by the enzyme endo‐1,3‐β‐glucosidase. However, genome‐wide analyses of callose GSL and endo‐1,3‐β‐glucosidase enzymes in fertile and sterile flower buds of Chinese cabbage have not yet been reported. Here, we show that delayed callose degradation at the tetrad stage may be the main cause of microspore abortion in Chinese cabbage with nuclear sterility near‐isogenic line ‘10L03’. Fifteen callose GSLs and 77 endo‐1,3‐β‐glucosidase enzymes were identified in Chinese cabbage. Phylogenetic, gene structural and chromosomal analyses revealed that the expansion occurred due to three polyploidization events of these two gene families. Expression pattern analysis showed that the GSL and endo‐1,3‐β‐glucosidase enzymes are involved in the development of various tissues and that the genes functionally diverged during long‐term evolution. Relative gene expression analysis of Chinese cabbage flowers at different developmental stages showed that high expression of the synthetic enzyme BraA01g041620 and low expression of AtA6‐homologous genes (BraA04g008040, BraA07g009320, BraA01g030220 and BraA03g040850) and two other genes (BraA10g020080 and BraA05g038340) for degrading enzymes in the meiosis and tetrad stages may cause nuclear sterility in the near‐isogenic line ‘10L03’. Overall, our data provide an important foundation for comprehending the potential roles of the callose GSL and endo‐1,3‐β‐glucosidase enzymes in regulating pollen development in Chinese cabbage.https://doi.org/10.1002/2211-5463.12685calloseChinese cabbageenzyme familygene expressionnuclear sterility |
| spellingShingle | Yanan Pu Lingyun Hou Yingqi Guo Ikram Ullah Yongping Yang Yanling Yue Genome‐wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis) FEBS Open Bio callose Chinese cabbage enzyme family gene expression nuclear sterility |
| title | Genome‐wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_full | Genome‐wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_fullStr | Genome‐wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_full_unstemmed | Genome‐wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_short | Genome‐wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_sort | genome wide analysis of the callose enzyme families of fertile and sterile flower buds of the chinese cabbage brassica rapa l ssp pekinensis |
| topic | callose Chinese cabbage enzyme family gene expression nuclear sterility |
| url | https://doi.org/10.1002/2211-5463.12685 |
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